Czech Journal of Genetics and Plant Breeding - Latest articles

Transcriptome analysis of alfalfa (Medicago sativa L.) roots reveals overwintering changes in different varieties

Yajun Ma, Guozhu Yang, Ruijun Duan, Xiaoan Li, Siheng Zeng, Yajun Yan, Ce Zheng, Yueming Hu

Czech J. Genet. Plant Breed., 2024, 60(2):97-104 | DOI: 10.17221/92/2023-CJGPB  

Low temperatures are one of the major abiotic stresses that affect alfalfa’s development and yield. Enhancing frost resistance through resistance-related genes is one of the most effective ways to address this issue in alfalfa. Therefore, exploring cold-resistant gene resources and the cultivation of cold-resistant alfalfa cultivars is inevitable in order to achieve high yield and quality. In this study, we conducted transcriptome profiling of roots obtained from two alfalfa genotypes, i.e., Qingda No.1 for freeze tolerance and Gannong No.9 for freeze sensitivity. We observed that Qingda No.1 had more lateral roots and a more developed root system...

Estimation of genetic diversity of sweet sorghum (Sorghum bicolor (L.) Moench) genotypes as a bioethanol source using SSRs markers

Herniwati Herniwati, Marcia Bunga Pabendon, Karuniawan Puji Wicaksono, Budi Waluyo, Eko Widaryanto

Czech J. Genet. Plant Breed., 2024, 60(2):86-96 | DOI: 10.17221/79/2023-CJGPB  

Sweet sorghum is a cereal crop that can potentially serve as a source for bioethanol production. This study aims to analyse the genetic diversity of promising genotypes of sweet sorghum at the molecular level. The genetic material consisted of 12 sweet sorghum genotypes. The genetic diversity estimated using 59 SSRs markers showed a polymorphism value of 0.48 and the coefficient of genetic diversity was classified as moderate. The unweighted pair group method arithmetic average (UPGMA) analysis assigned the tested genotypes into three major clusters with a similarity coefficient level of 0.596. This indicates that the genetic similarity of the tested...

Impact of selected antimitotic substances on doubled haploid and polyploid regeneration in microspore cultures of swede (Brassica napus ssp. napobrassica (L.) Hanelt)

Miroslav Klíma, Yamen H. Shmeit, Pavel Kopecký, Pavel Vítámvás, Klára Kosová, Ilja T. Prášil, Eloy Fernández-Cusimamani

Czech J. Genet. Plant Breed., 2024, 60(2):79-85 | DOI: 10.17221/84/2023-CJGPB  

Several antimitotic agents were tested in three embryogenic doubled haploid (DH) lines of swede (Brassica napus ssp. napobrassica). No effect on embryogenesis was observed at the given concentrations of colchicine (5 μmol/L or 50 mg/L) and treatment time of 24 hours. Flow cytometric analysis of microspore embryos revealed a significant increase in the percentage of DH compared to the control for all substances: ethalfluralin, 62.2%; trifluralin, 58.3%; colchicine, 56.1%; amiprophos-methyl, 54.4%; pronamide, 35.0%; control, 13.3%. The occurrence of pure tetraploids was low (0–5%), mixoploids – especially n+2n...

Malting quality molecular markers for barley breeding

Leona Leišová-Svobodová, Vratislav Psota, Marta Zavřelová, Martin Kříž, Pavel Mařík, Zdeněk Nesvadba

Czech J. Genet. Plant Breed., 2024, 60(2):70-78 | DOI: 10.17221/72/2023-CJGPB  

Barley is one of the world’s most important crops. Barley is used for both food and feed and is important for the production of malt. Malt quality is a complex function of barley genetics, environmental conditions during barley growth, and the technological aspects of the malting process. Due to the high heritability of more than half of the malting parameters, barley can be bred for malting quality. Marker-assisted selection (MAS) is a good way to speed up the breeding process. In this study, nine molecular markers were used to screen 115 barley varieties and breeding lines over a four-year period. The results were compared with malting quality...

Exploring strigolactones for inducing abiotic stress tolerance in plants

Mohd. Kamran Khan, Anamika Pandey, Mehmet Hamurcu, Tomáš Vyhnánek, Sajad Majeed Zargar, Abdullah Kahraman, Ali Topal, Sait Gezgin

Czech J. Genet. Plant Breed., 2024, 60(2):55-69 | DOI: 10.17221/88/2023-CJGPB  

Strigolactones (SLs) are a comparatively novel class of phytohormones that are involved in the maintenance of plant architecture with key regulation of lateral branching. They are also reported to play an important role in regulating plant responses to various abiotic stresses. The potential of SLs to improve crop resilience and productivity needs to be effectively translated into agriculture. Therefore, both the endogenous biosynthesis of SLs and their exogenous application need to be thoroughly investigated to understand and exploit the mechanism underlying the alleviation of abiotic stress responses. Although several reviews have emphasised the...

List of Reviewers 2023

Editorial Department

Czech J. Genet. Plant Breed., 2024, 60(1)  

Induction of direct somatic embryogenesis and genetic stability of somatic embryo-derived plants of broccoli

Suzana Pavlović, Jelena Damnjanović, Zdenka Girek, Lela Belić, Milan Ugrinović

Czech J. Genet. Plant Breed., 2024, 60(1):50-54 | DOI: 10.17221/26/2023-CJGPB  

The influence of the developmental stage of zygotic embryos and the composition and pH of the Gamborg induction medium B5 on the initiation and development of somatic embryos was investigated. The optimal medium was B5 medium with a pH value of 5.0 and without plant growth regulator, at which the highest frequency of somatic embryogenesis (56.67%) and the highest average number of somatic embryos per explant (3.35) were achieved. Somatic embryos appeared directly on the hypocotyls of the explants, without the callus stage. On zygotic embryos in the early cotyledonary phase, three times higher regeneration was achieved compared to larger embryos in the...

Genomic SSR marker development in lentil (Lens culinaris Medik.) and assessment of cross-species/genera transferability to related legumes

Melike Bakır, Şehriban Demir, Cebrail Yıldırım, Abdullah Kahraman

Czech J. Genet. Plant Breed., 2024, 60(1):42-49 | DOI: 10.17221/57/2023-CJGPB  

Simple sequence repeat (SSR) markers are valuable genetic and genomic research tools and are extensively used in major crops. However, a paucity of available molecular markers in lentils (Lens culinaris Medik.) has significantly hindered genomic studies in this vital legume crop. In this study, we developed 33 new SSR markers for lentils using an enriched genomic library and tested their polymorphism in 10 lentil cultivars. We found that 16 (48.5%) SSR markers were polymorphic. The expected heterozygosity values of the polymorphic SSR markers ranged from 0.095 to 0.820, while observed heterozygosity values varied between 0.100 and 1.00. In addition,...

Verification of resistance loci pyramiding in popular interspecific grape varieties using SSR markers

Martin Hádlík, Miroslav Baránek, Ivana Flajšingerová, Kateřina Baránková

Czech J. Genet. Plant Breed., 2024, 60(1):34-41 | DOI: 10.17221/43/2023-CJGPB  

Fungal pathogens Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni (downy mildew) and Erysiphe necator Schwein. (powdery mildew) represent the biggest threats for grape growers worldwide. Under suitable conditions, these pathogens can spread very quickly through vineyards and cause significant damage. The most ecological way to reduce the possibility of infection in vineyards is growing interspecific genotypes which are able to suppress these pathogens and stop the spread of infection. With this in mind, 9 international and 11 Czech grapevine genotypes were analysed in order to genotype the resistance loci present in them....

Development of PCR-based markers associated with powdery mildew resistance using bulked segregant analysis  (BSA-seq) in melon

Yawo Mawunyo Nevame Adedze, Xia Lu, Wenyi Fan, Wenting Zhang, Xue Yang, Zhijun Deng, Md. Amirul Alam, Guangli Xu, Lihua Zhang, Wenhu Li

Czech J. Genet. Plant Breed., 2024, 60(1):25-33 | DOI: 10.17221/40/2023-CJGPB  

Powdery mildew (PM) is a fungus that causes disease in both the field and the greenhouse. Utilizing resistant cultivars is the most effective approach of disease management. To develop insertion-deletion (InDel) markers associated to this trait, the whole genomes of the PM resistant line M17050 (P1) and the PM-susceptible line 28-1-1 (P2) were sequenced. A total of 1 200 InDels, with an average of 100 markers per chromosome, were arbitrarily chosen from the sequencing data for experimental validation. One hundred InDel markers were ultimately selected due to their informative genetic bands. Further, an F₂ segregating population of melons...

Genetic diversity among coloured cotton genotypes in relation to their fibre colour and ploidy level based on SSR markers

Revanasiddayya, Jayaprakash Mohan Nidagundi, Bashasab Fakrudin, Prakash Kuchanur, Lingappa Neelagiri Yogeesh, Shivanand Hanchinal, Talagunda Chandrashekar Suma, Gururaj Sunkad, Bharamappanavara Muralidhara, Doddamani Maheshkumar, Channabasava, Patil Sudha, Shivamurthy Rashmi, Virupapura Cholaraya Raghavendra

Czech J. Genet. Plant Breed., 2024, 60(1):12-24 | DOI: 10.17221/12/2023-CJGPB  

Genetic diversity is referred to as any variation at the phenotypic, DNA or genomic level of an individual, population or species. The appraisal of diversity is important to understand its pattern and evolutionary relationships between germplasms or genotypes, which will aid in sampling the genetic resources in a more systematic manner for conservation and crop improvement. The present study employed 50 simple sequence repeat (SSR) markers linked to the yield and fibre quality/colour traits for estimating the genetic diversity in 33 cotton genotypes of diploid and tetraploid species differing in fibre colour. The diversity analysis was performed in GenAlEx...

Genotypic and environmental variability and stability of seed yield, oil content and fatty acids in high-oleic and high-linoleic safflower (Carthamus tinctorius L.) lines and cultivars

Sabri Erbaş, Hasan Baydar, Halil Hatipoğlu, Hasan Koç, Metin Babaoğlu, Arzu Köse

Czech J. Genet. Plant Breed., 2024, 60(1):1-11 | DOI: 10.17221/113/2022-CJGPB  

The present study was to determine the genotypic and environmental variability and stability in seed yield, oil content, oil yield, oleic and linoleic acid of 10 safflower lines derived from a cross of Dinçer 5-18-1 × Montola 2000 together with six cultivars under six environments at five locations. The effects of genotypes, environments and genotype × environment interactions were highly significant (P < 0.01) for seed yield and oil content. Averaged across all environments, the seed yield was lowest in the cultivar Olas (2 352 kg/ha), and highest in the line Bay-Er 5 (2 869 kg/ha). According to mean (x_i) and regression...

Index of Volume 59_Authors Index

Editorial Department

Czech J. Genet. Plant Breed., 2023, 59(4):I-IV  

Identification and functional analysis of the HvWRKY1 gene associated with Qingke (Hordeum vulgare L. var. nudum Hook. f.) leaf stripe disease

Gang Jing, Youhua Yao, Likun An, Yongmei Cui, Yixiong Bai, Xin Li, Xiaohua Yao, Kunlun Wu

Czech J. Genet. Plant Breed., 2023, 59(4):263-277 | DOI: 10.17221/14/2023-CJGPB  

To explore the role of WRKY transcription factors (TFs) in the resistance process of Qingke (Hordeum vulgare L. var. nudum Hook. f.), leaves of the leaf stripe disease-resistant variety Kunlun 14 and the susceptible variety Z1141 were sequenced by transcriptome sequencing (RNA-seq). A differentially expressed gene HvnWKRY1 was identified, and its disease-resistance function was preliminarily analysed. The result showed that the open reading frame (ORF) of the gene was 1 062 bp and encoded 354 amino acids. It contained the conserved WRKY domain (273–351) and belonged to the WRKY protein family. The phylogenetic tree...

Crossing possibility for breeding promising orange-fleshed sweetpotato genotypes in Benin

Fernand Silvère Sohindji, Florent J.-B. Quenum, Nicodème V. Fassinou-Hotegni, Adechina Adékounlé Oke, Charlotte O. A. Adje, Enoch G. Achigan-Dako

Czech J. Genet. Plant Breed., 2023, 59(4):253-262 | DOI: 10.17221/104/2022-CJGPB  

Orange-fleshed sweetpotato (OFSP) is a sustainable and inexpensive source of vitamin A that contributes to food and nutritional security in developing countries. Its adoption is low due to unconformity of the variety with community food culture, limited cultivar selection and breeding studies. The current study aimed at breeding of promising OFSP genotypes in Benin using three parental lines. Two hundred and seventy-four seeds and one hundred and six genotypes were obtained through biparental crosses of Tio-joe (imported orange-fleshed variety), Vobodouaho (local white-fleshed variety) and ACAB220 (local orange-fleshed variety). The phenotypic diversity...

Transcriptome profiling of Sorghum bicolor reveals cultivar-specific molecular signatures associated with starch and phenolic compounds biosyntheses and accumulation during sorghum grain development

Li Wang, Derang Ni, Fan Yang, Lin Lin, Yubo Yang, Chongde Sun, Xingqian Ye, Jinping Cao, Xiangli Kong

Czech J. Genet. Plant Breed., 2023, 59(4):235-252 | DOI: 10.17221/16/2023-CJGPB  

Sorghum is an important crop, and starch and phenolic compounds are major and important components in the sorghum grain. However, the underlying critical genetic elements contributing to the rich portfolio of nutrients in sorghum grains are largely unknown. Transcriptomic methods were employed to characterize the expression patterns at five different grain developmental stages of Hongyingzi (an important brewing sorghum), and another two grain sorghums, Jinuoliang 1 and Hongliangfeng 1, for comparison. The uniquely expressed genes were identified at each developmental stage of Hongyingzi when compared with the other two sorghum cultivars. The co-regulated...

Identification of genes for leaf rust resistance in seedlings of wheat cultivars from the Yellow-Huai Basin in China and slow rusting observations in field trials

Huali Yang, Wenda Diao, Xiaocui Yan, Takele-Weldu Gebrewahid, Zaifeng Li, Zhanjun Yao

Czech J. Genet. Plant Breed., 2023, 59(4):219-234 | DOI: 10.17221/9/2023-CJGPB  

Wheat leaf rust is a devastating disease worldwide. Identification of leaf rust resistance genes in seedlings and of genes for slow rusting are important in resistance breeding and for gene deployment to control the disease. A total of 108 wheat cultivars from the Beijing and Shandong province and a set of 36 differentials, mostly near-isogenic lines in the background of Thatcher with known leaf rust resistance genes, were tested with 20 Puccinia triticina pathotypes  (FHJS1, FGBQ,PGJQ, SHJT, FHGQ, PHTT1, FHGQ, FHGQ, PHJS, THSM, FHSQ, PHST, PRSQ, FNTQ, PHGM, KHGQ, PHTT2, TGTT, FHJS2, NHHT) at the seedling stage in the greenhouse. The cultivars...

Selection of suitable reference genes in Paulownia fortunei (Seem.) Hemsl. under different tissues and abiotic stresses for qPCR normalization

Jiang Su, Kanghua Xian, Chuanming Fu, Jinxiang He, Baojun Liu, Ningzhen Huang

Czech J. Genet. Plant Breed., 2023, 59(4):205-218 | DOI: 10.17221/72/2022-CJGPB  

By choosing appropriate candidate reference genes (CRGs) and standardizing qPCR data, more accurate experimental data can be obtained. Herein, the expression stability of alpha-tubulin1 (TUA1), beta-tubulin (TUB), beta-tubulin 1 (TUB1), beta-tubulin 5 (TUB5), actin 1 (ACT1), actin 97 (ACT97), molecular chaperone dnaj (DNAJ), adenine phosphoribosyl transferase (APT), and histone H4 (HIS4) genes from Paulownia fortunei (Seem.) Hemsl. under different experimental conditions (different tissues, drought, salinity, Cd, and...

Mapping of genomic regions associated with dwarfing and the determinate growth habit in horsegram (Macrotyloma uniflorum)

Mala Ram Modi, Megha Katoch, Nisha Thakur, Manisha Gautam, Sunny Choudhary, Rakesh Kumar Chahota

Czech J. Genet. Plant Breed., 2023, 59(4):196-204 | DOI: 10.17221/119/2022-CJGPB  

Horsegram (Macrotyloma uniflorum) – an important, self-pollinated food legume, however due to limited genomic and genetic resources the genetic improvement could not be achieved as compare to other major legumes. Our work aims at finding novel microsatellite markers and their use for the construction of a linkage map from 157 individuals of F₉ recombinant inbred lines (RILs) of horsegram. The determinate growth habit and plant height are important traits for its suitability for different cropping systems. The genotypic data were generated by screening 2 395 molecular markers, of which 600 (25.05 %) polymorphic markers were selected....

The cytochrome P450 gene GhCYP94C1 is involved in drought stress in upland cotton (Gossypium hirsutum L.)

Lijiao Gu, Pengyun Chen, Shuxun Yu

Czech J. Genet. Plant Breed., 2023, 59(4):189-195 | DOI: 10.17221/108/2022-CJGPB  

Cytochrome P450 proteins belong to one of the largest families of enzyme proteins in plants and play important roles in plant growth and development and the stress response. In our previous studies, a cytochrome P450 gene, GhCYP94C1 (cytochrome P450 94C1), was functionally characterized as a positive regulator of seed germination, main root elongation and early flowering. However, whether the gene has other potential functions remains to be further explored. In our study, expression analysis showed that GhCYP94C1 was highly expressed in roots and was suppressed by drought treatment. Endogenous silencing of GhCYP94C1 via virus-induced...

Inheritance and allelism of brown midrib trait introgressed in agronomically promising backgrounds in pearl millet (Pennisetum glaucum (L.) R. Br.)

Shashi Kumar Gupta^*, Ponnaiah Govintharaj

Czech J. Genet. Plant Breed., 2023, 59(3):176-187 | DOI: 10.17221/93/2022-CJGPB  

The lignin content of forage crops significantly affects the livestock’s forage intake and its dry matter digestibility. The brown midrib (bmr) trait is known for its association with a reduced lignin content and increased digestibility. Pearl millet inbreds having the bmr trait were derived from two populations ICMV bmr and WRajPop bmr via continued selfing up to 6–8 generations. The results from the crosses revealed the presence of the same single recessive gene that controlled the bmr in both the populations. The stable expression of the bmr gene in the new genetic background indicated that there...

Morpho-genetic characterization of diploid and tetraploid taro (Colocasia esculenta L. Schott) cv. Kaliurang – An Indonesian local cultivar

Dyah Retno Wulandari, Andri Fadillah Martin, Tri Muji Ermayanti, Khalisa Aini Sinaga, Diah Ratnadewi

Czech J. Genet. Plant Breed., 2023, 59(3):169-175 | DOI: 10.17221/97/2022-CJGPB  

Kaliurang is one of the local taro cultivars in Indonesia, potentially due to its high productivity and delicacy. This study analysed morpho-genetic variation between one diploid and three tetraploid Kaliurang taro clones from in vitro polyploid induction after plantlet acclimatization in the greenhouse for 6 weeks. Plants’ morphological characters were analysed using the unweighted pair group method with arithmetic mean (UPGMA) method based on 50 characters according to the taro descriptor from the International Plant Genetic Resources Institute (IPGRI). Furthermore, a morphometric characterization based on the Euclidean distance of 17...

Complete chloroplast genome sequence and characteristics analysis of Qingda no.1 alfalfa (Medicago sativa L. cv. Qingda no.1)

Yuling Ren, Yajun Ma, Xue Li, Xiaoan Li, Guozhu Yang, Ping Li

Czech J. Genet. Plant Breed., 2023, 59(3):160-168 | DOI: 10.17221/71/2022-CJGPB  

Medicago sativa is the most widely cultivated forage legume and one of the most economically valuable crops throughout the world. Qingda no.1 (Medicago sativa L. cv. Qingda no.1) is an excellent alfalfa local variety with strong cold, drought and salt resistance in the three rivers source area of Qinghai. In this study, the whole chloroplast (cp) genome of Qingda no.1 was sequenced, assembled and its structure was analysed by the Illumina high-throughput sequencing technology. The results showed that the chloroplast genome of Qingda no.1 exhibits no obvious typical quadripartite structure; the total length of the chloroplast genome is...

Assessment of genetic variation and population structure in Iraqi barley accessions using ISSR, CDDP, and SCoT markers

Nawroz Tahir, Djshwar Lateef, Kamaran Rasul, Didar Rahim, Kamil Mustafa, Shokhan Sleman, Avin Mirza, Rebwar Aziz

Czech J. Genet. Plant Breed., 2023, 59(3):148-159 | DOI: 10.17221/112/2022-CJGPB  

The objective of this study was to investigate the diversity of 59 accessions of barley using inter simple sequence repeat (ISSR), conserved DNA-derived polymorphism (CDDP), and start codon targeted (SCoT) markers. A total of 391 amplified polymorphic bands were generated using 44 ISSR, 9 CDDP, and 12 SCoT primers that produced 255, 35, and 101 polymorphic bands, respectively. The average values of gene diversity were 0.77, 0.67, and 0.81 for ISSR, CDDP, and SCoT markers, respectively. The mean values of polymorphism information content for ISSR, CDDP and SCoT markers were 0.74, 0.63, and 0.80 respectively. The discrimination power of the three approaches...

Effect of long-term storage on the change in the expression of selected Mal d 1 gene isoforms in the apple cultivar Opal^®

Ivona Žďárská, Radek Čmejla

Czech J. Genet. Plant Breed., 2023, 59(3):141-147 | DOI: 10.17221/102/2022-CJGPB  

Apples are available all year round since they can be stored for long time. However, the expression of the major apple allergen Mal d 1 can increase in the fruit during storage and incease so the risk of allergies in sensitive consumers. Therefore, we studied the change in the expression of the Mal d 1 gene during storage in the modern cultivar UEB 32642 (known under the brand name Opal^®) and the cultivar Gala Brookfield (cv. Gala) as a reference. The cultivars were stored under two different conditions, ultra-low oxygen (ULO) and standard cold conditions. The gene expression was evaluated both in fresh fruits and in fruits stored...

A preliminary study on the root-knot nematode resistance of a cherry plum cultivar Mirabolano 29C

Jia Liu, Jin Zhu, Hongwen Li, Deyong Luo, Jialian Xie, Huajia Li, Shuo Liu, Yuping Zhang, Lijuan Chen, Xiaodou Xie, Dong Wang, Ke Li, Meiying Yao, Guowei Zhang

Czech J. Genet. Plant Breed., 2023, 59(3):133-140 | DOI: 10.17221/111/2022-CJGPB  

Grafting is the most important means of breeding peach, plum, apricot, and other fruit trees, and the selection of the rootstock is crucial to the quality of the grafting and the yield of the products. The traditional commonly used peach rootstock is susceptible to root-knot nematode infections, resulting in a decreased yield, while a variety of cherry plum rootstocks, Mirabolano 29C, is resistant to root-knot nematode. In this study, root-knot nematode infection experiments on seedlings of traditional peach rootstocks and Mirabolano 29C confirmed that Mirabolano 29C was indeed more resistant to root-knot nematodes. At the same time, we compared the...

Whole genome identification of CBF gene families and expression analysis in Vitis vinifera L.

Xiang Fang, Yiling Lin, Chun Chen, Tariq Pervaiz, Xicheng Wang, Hefei Luo, Jinggui Fang, Lingfei Shangguan

Czech J. Genet. Plant Breed., 2023, 59(3):119-132 | DOI: 10.17221/82/2022-CJGPB  

The CBF (C-repeat binding factors) genes play important roles in response to abiotic stress and environmental changes. In the present study, a total of 18 CBF genes were identified from a grapevine. Their domains, phylogenetics, and collinearity were analysed. The results revealed, that 18 VviCBF genes were distributed on 10 chromosomes unevenly in the grape genome. Promoter data analysis showed that the CBF gene has many cis-acting elements related to plant growth and development, light response, hormone, and abiotic stress response. We found that six VviCBF genes including, VviCBF5, VviCBF13,...

List of Field Crop Varieties Registered in the Czech Republic in 2022

Tomáš Mezlík

Czech J. Genet. Plant Breed., 2023, 59(2):117-118 | DOI: 10.17221/22/2023-CJGPB  

Delineation of inbred lines of Indian mustard into diverse gene pools based on agro-morphological traits

Kunwar Harendra Singh, Guman Singh, Lal Singh, Nashra Aftab, Ajay K. Thakur

Czech J. Genet. Plant Breed., 2023, 59(2):109-116 | DOI: 10.17221/75/2022-CJGPB  

The present study was conducted to explore the variability generated through recombination breeding for nine economically important traits in Indian mustard (Brassica juncea L. Czern & Coss.). Delineation of the inbred lines to different gene pools, based on genetic diversity, enables their utilisation in hybrid breeding. The unweighted pair group method with arithmetic mean (UPGMA) and a Euclidean distance matrix was used to delineate the inbred lines to clusters. The variability was studied using the range and coefficients of variation of the traits. Significant variability was observed for all studied traits except for oil content and...

QTL localisation of seed-related traits in Tibetan hulless barley based on a high-density single-nucleotide polymorphism genetic map

Yan Wang, Xiaohua Yao, Youhua Yao, Yixiong Bai, Likun An, Xin Li, Yongmei Cui, Kunlun Wu

Czech J. Genet. Plant Breed., 2023, 59(2):95-108 | DOI: 10.17221/74/2022-CJGPB  

The effective use of high-quality and high-yielding germplasm resources is of great importance for the development of hulless barley varieties. Therefore, the search for such resources has long been a goal of the breeding community. In this study, a genotyping-by-sequencing (GBS) analysis was performed on an F₂ (Nierumuzha × Kunlun 10) population. A high-density genetic map of hulless barley was constructed, which contained 1 475 efficient single-nucleotide polymorphism markers with 7 052 bin markers. The total length of the seven chromosomes was 1 139.4 cM, with an average marker distance of 0.59 cM. Based on this high-density linkage map,...