Czech Journal of Genetics and Plant Breeding, 2025 (vol. 61), issue 2
Association analysis of the molecular characteristics and floral traits of Iris × germanicaOriginal Paper
Feng Tian, Ya Tian, Fang Yu, Jinsen Qian, Feijian Wang, Xue Li, Tongyin Li, Xiaofei Zhang, Dazhuang Huang, Xiaojie Zhao
Czech J. Genet. Plant Breed., 2025, 61(2):55-66 | DOI: 10.17221/93/2024-CJGPB 
Iris × germanica L. (bearded iris) is a popular ornamental plant with numerous commercially important cultivars; however, little is known about the genetic diversity and population structure of the species, as limited DNA markers have been explored. In this study, 34 722 expressed sequence tag (EST)-simple sequence repeat (SSR) loci were identified from RNA sequencing data. The most abundant SSR motifs belonged to the tri-nucleotide type, of which the most common were AGG/CCT followed by AAG/CTT. Overall, 50 primer pairs derived from these EST-SSRs were randomly selected and synthesized, and 22 primer pairs with good polymorphism effects...
Agronomic and pod traits in relation to pod shattering in cultivated soybeansOriginal Paper
Bingjie Tu, Qingying Zhang, Xiaobing Liu, Shaopeng Yu, Nan Xu, Jia Liu, Changkai Liu
Czech J. Genet. Plant Breed., 2025, 61(2):67-76 | DOI: 10.17221/119/2024-CJGPB
Pod dehiscence or pod shattering from mature soybean (Glycine max L.) is one of the most outstanding disadvantages in domesticated cultivars. Pod shattering in relation to 16 quantitative traits and 3 qualitative traits among 140 cultivars of vegetable soybeans, grain soybeans and small-grain soybeans was evaluated over two years. We found the pod shattering percentage is positively correlated with the number of productive branches, pod width, pod length, pod area, 100-seed weight, 1-seeded-pod percentage, 2-seeded-pod percentage and seed protein content, but negatively correlated with the plant height, pod height at the bottom, number of nodes...
Ectopic expression of the cowpea (Vigna unguiculata) VuCERK1 gene confers enhanced resistance to Pto DC3000 hrcC- in ArabidopsisOriginal Paper
Weida Chen, Lifen Gao, Gao Chen, Ting Yang, Zixin Zhao, Wenhao Xia, Niannian Fan, Siming Chen
Czech J. Genet. Plant Breed., 2025, 61(2):77-85 | DOI: 10.17221/112/2024-CJGPB
Pattern recognition receptors (PRRs) play multiple roles in plants. As a kind of PRRs, chitin elicitor receptor kinase 1 (CERK1) proteins were reported to function in plant resistance to fungal and bacterial pathogens, and tolerance to salt stress. In this study, a predicted cowpea CERK1 homologous gene, designated as VuCERK1, was identified by database search. VuCERK1 protein contains 618 amino acid residues, with a predicted molecular mass of 67.5 kDa and a predicted isoelectric point of 5.04. VuCERK1 shows 58% and 60% sequence identity with AtCERK1 and OsCERK1, respectively. VuCERK1 also shows similar subcellular pattern with AtCERK1...
Characterization of carotenoid profiles and presence of functional markers in sub-tropical maize (Zea mays L.) inbred linesOriginal Paper
Peter Amoah, Victor O. Adetimirin, Bismark Anokye, Oluyinka J. llesanmi, Nnanna Unachukwu, Elisabeth Ivania Aporva
Czech J. Genet. Plant Breed., 2025, 61(2):86-99 | DOI: 10.17221/143/2024-CJGPB
Biofortification provides a sustainable, pragmatic strategy to address the lack of vitamin A and the associated health complications. The objectives of the study encompassed the quantification of the carotenoid content of 147 maize inbred lines, the identification of variable regions within the ZEP1 gene, the correlation of these observed variances in the presence of this gene with carotenoid content, and the identification of lines harbouring the favourable alleles of the crtRB1 gene. The observed correlations among the carotenoids synthesised by distinct branches of the biosynthetic process were both significant and positive. Utilising...
Reciprocal hybridisation of Aloe species (Aloe arborescens with A. vera) and their characterisation in a highland region of VenezuelaOriginal Paper
José Imery-Buiza, Wendy Ozols-Narbona
Czech J. Genet. Plant Breed., 2025, 61(2):100-109 | DOI: 10.17221/7/2025-CJGPB
Aloe vera and A. arborescens are succulent plants widely used in cosmetics, pharmaceuticals, and food supplements. The objective of this study was to perform interspecific crosses and agronomically characterise three genotypes cultivated in a Venezuelan highland region (altitude 1 727 m, 13–17.9 °C). Successful hybridisation was achieved only when A. arborescens pollen (P1) was used on A. vera pistils (P2), whereas the reciprocal cross was largely unsuccessful. Hybrid seed germination reached 11.86%, and adult hybrids exhibited significant vegetative superiority over P1, particularly in leaf volume, leaf weight, and...
List of varieties registered in the Czech Republic in 2024New Varieties
Tomá¹ Mezlík
Czech J. Genet. Plant Breed., 2025, 61(2):110-112 | DOI: 10.17221/16/2025-CJGPB