Czech Journal of Genetics and Plant Breeding, 2023 (vol. 59), issue 4
Index of Volume 59_Authors IndexIndex
Editorial Department
Czech J. Genet. Plant Breed., 2023, 59(4):I-IV 
The cytochrome P450 gene GhCYP94C1 is involved in drought stress in upland cotton (Gossypium hirsutum L.)Original Paper
Lijiao Gu, Pengyun Chen, Shuxun Yu
Czech J. Genet. Plant Breed., 2023, 59(4):189-195 | DOI: 10.17221/108/2022-CJGPB
Cytochrome P450 proteins belong to one of the largest families of enzyme proteins in plants and play important roles in plant growth and development and the stress response. In our previous studies, a cytochrome P450 gene, GhCYP94C1 (cytochrome P450 94C1), was functionally characterized as a positive regulator of seed germination, main root elongation and early flowering. However, whether the gene has other potential functions remains to be further explored. In our study, expression analysis showed that GhCYP94C1 was highly expressed in roots and was suppressed by drought treatment. Endogenous silencing of GhCYP94C1 via virus-induced...
Mapping of genomic regions associated with dwarfing and the determinate growth habit in horsegram (Macrotyloma uniflorum)Original Paper
Mala Ram Modi, Megha Katoch, Nisha Thakur, Manisha Gautam, Sunny Choudhary, Rakesh Kumar Chahota
Czech J. Genet. Plant Breed., 2023, 59(4):196-204 | DOI: 10.17221/119/2022-CJGPB
Horsegram (Macrotyloma uniflorum) – an important, self-pollinated food legume, however due to limited genomic and genetic resources the genetic improvement could not be achieved as compare to other major legumes. Our work aims at finding novel microsatellite markers and their use for the construction of a linkage map from 157 individuals of F9 recombinant inbred lines (RILs) of horsegram. The determinate growth habit and plant height are important traits for its suitability for different cropping systems. The genotypic data were generated by screening 2 395 molecular markers, of which 600 (25.05 %) polymorphic markers were selected....
Selection of suitable reference genes in Paulownia fortunei (Seem.) Hemsl. under different tissues and abiotic stresses for qPCR normalizationOriginal Paper
Jiang Su, Kanghua Xian, Chuanming Fu, Jinxiang He, Baojun Liu, Ningzhen Huang
Czech J. Genet. Plant Breed., 2023, 59(4):205-218 | DOI: 10.17221/72/2022-CJGPB
By choosing appropriate candidate reference genes (CRGs) and standardizing qPCR data, more accurate experimental data can be obtained. Herein, the expression stability of alpha-tubulin1 (TUA1), beta-tubulin (TUB), beta-tubulin 1 (TUB1), beta-tubulin 5 (TUB5), actin 1 (ACT1), actin 97 (ACT97), molecular chaperone dnaj (DNAJ), adenine phosphoribosyl transferase (APT), and histone H4 (HIS4) genes from Paulownia fortunei (Seem.) Hemsl. under different experimental conditions (different tissues, drought, salinity, Cd, and...
Identification of genes for leaf rust resistance in seedlings of wheat cultivars from the Yellow-Huai Basin in China and slow rusting observations in field trialsOriginal Paper
Huali Yang, Wenda Diao, Xiaocui Yan, Takele-Weldu Gebrewahid, Zaifeng Li, Zhanjun Yao
Czech J. Genet. Plant Breed., 2023, 59(4):219-234 | DOI: 10.17221/9/2023-CJGPB
Wheat leaf rust is a devastating disease worldwide. Identification of leaf rust resistance genes in seedlings and of genes for slow rusting are important in resistance breeding and for gene deployment to control the disease. A total of 108 wheat cultivars from the Beijing and Shandong province and a set of 36 differentials, mostly near-isogenic lines in the background of Thatcher with known leaf rust resistance genes, were tested with 20 Puccinia triticina pathotypes (FHJS1, FGBQ,PGJQ, SHJT, FHGQ, PHTT1, FHGQ, FHGQ, PHJS, THSM, FHSQ, PHST, PRSQ, FNTQ, PHGM, KHGQ, PHTT2, TGTT, FHJS2, NHHT) at the seedling stage in the greenhouse. The cultivars...
Transcriptome profiling of Sorghum bicolor reveals cultivar-specific molecular signatures associated with starch and phenolic compounds biosyntheses and accumulation during sorghum grain developmentOriginal Paper
Li Wang, Derang Ni, Fan Yang, Lin Lin, Yubo Yang, Chongde Sun, Xingqian Ye, Jinping Cao, Xiangli Kong
Czech J. Genet. Plant Breed., 2023, 59(4):235-252 | DOI: 10.17221/16/2023-CJGPB
Sorghum is an important crop, and starch and phenolic compounds are major and important components in the sorghum grain. However, the underlying critical genetic elements contributing to the rich portfolio of nutrients in sorghum grains are largely unknown. Transcriptomic methods were employed to characterize the expression patterns at five different grain developmental stages of Hongyingzi (an important brewing sorghum), and another two grain sorghums, Jinuoliang 1 and Hongliangfeng 1, for comparison. The uniquely expressed genes were identified at each developmental stage of Hongyingzi when compared with the other two sorghum cultivars. The co-regulated...
Crossing possibility for breeding promising orange-fleshed sweetpotato genotypes in BeninOriginal Paper
Fernand Silvère Sohindji, Florent J.-B. Quenum, Nicodème V. Fassinou-Hotegni, Adechina Adékounlé Oke, Charlotte O. A. Adje, Enoch G. Achigan-Dako
Czech J. Genet. Plant Breed., 2023, 59(4):253-262 | DOI: 10.17221/104/2022-CJGPB
Orange-fleshed sweetpotato (OFSP) is a sustainable and inexpensive source of vitamin A that contributes to food and nutritional security in developing countries. Its adoption is low due to unconformity of the variety with community food culture, limited cultivar selection and breeding studies. The current study aimed at breeding of promising OFSP genotypes in Benin using three parental lines. Two hundred and seventy-four seeds and one hundred and six genotypes were obtained through biparental crosses of Tio-joe (imported orange-fleshed variety), Vobodouaho (local white-fleshed variety) and ACAB220 (local orange-fleshed variety). The phenotypic diversity...
Identification and functional analysis of the HvWRKY1 gene associated with Qingke (Hordeum vulgare L. var. nudum Hook. f.) leaf stripe diseaseOriginal Paper
Gang Jing, Youhua Yao, Likun An, Yongmei Cui, Yixiong Bai, Xin Li, Xiaohua Yao, Kunlun Wu
Czech J. Genet. Plant Breed., 2023, 59(4):263-277 | DOI: 10.17221/14/2023-CJGPB
To explore the role of WRKY transcription factors (TFs) in the resistance process of Qingke (Hordeum vulgare L. var. nudum Hook. f.), leaves of the leaf stripe disease-resistant variety Kunlun 14 and the susceptible variety Z1141 were sequenced by transcriptome sequencing (RNA-seq). A differentially expressed gene HvnWKRY1 was identified, and its disease-resistance function was preliminarily analysed. The result showed that the open reading frame (ORF) of the gene was 1 062 bp and encoded 354 amino acids. It contained the conserved WRKY domain (273–351) and belonged to the WRKY protein family. The phylogenetic tree...