Czech Journal of Genetics and Plant Breeding, 2022 (vol. 58), issue 1

Isolation, sequencing of the HvnHID gene and its role in the purple-grain colour development in Tibetan hulless barleyOriginal Paper

Xiaohua Yao, Leping Su, Youhua Yao, Likun An, Yixiong Bai, Xin Li, Kunlun Wu

Czech J. Genet. Plant Breed., 2022, 58(1):1-9 | DOI: 10.17221/34/2021-CJGPB  

2-hydroxyisoflavanone dehydratase (HID) plays an important role in isoflavone biosynthesis. In this study, HID was isolated from the seeds of the purple-grained Tibetan hulless barley variety Nerumuzha and the white-grained variety Kunlun 10. The HvnHID gene includes the 981 bp open reading frame and encodes a protein of 327 amino acids. It has a typical Abhydrolase_3 domain (78-306) and belongs to the carboxylesterase (CXE) family of the Abhydrolase_3 (α/β hydrolase) superfamily. There are eight nucleotide differences in the HvnHID coding sequence and two amino acid differences (one in the Abhydrolase_3 domain)...

Comparative analysis of late blight resistance R genes and their coding proteins in some potato genotypesOriginal Paper

Heba Amin Mahfouze, Osama Ezzat El-Sayed

Czech J. Genet. Plant Breed., 2022, 58(1):10-20 | DOI: 10.17221/49/2021-CJGPB  

Late blight (LB) disease can cause potato yield losses in both Egypt and the world. Therefore, the structural analysis of resistance (R) genes responsible for LB resistance will help in understanding their functions. This work aimed to identify the variations between the dominant and recessive alleles of two genes, R3a and R8 at the nucleotide and amino acid levels in five potato genotypes. Two genes of R3a and R8 representing the broad-spectrum LB resistance were amplified by specific primers, which gave one amplicon of 194 and 220 bp of each gene, respectively. Two fragments were sequenced after purification...

A 31-bp indel localised in the 5' untranslated region of OsSUT3 affects the gene expression and rice (Oryza sativa L.) pollen developmentOriginal Paper

Chunlong Zhang, Qiuping Li, Hong Yang, Tuo Wang, Juan Li, Jiancheng Wen, Shoulin Jin, Zhonglin Zhang, Lijuan Chen, Dandan Li

Czech J. Genet. Plant Breed., 2022, 58(1):21-28 | DOI: 10.17221/66/2021-CJGPB  

OsSUT genes have been demonstrated to be relevant for diverse biological processes in rice. In this study, we identified the close relationship between a 31-bp insertion in a 5' untranslated region (5' UTR) of the OsSUT3 gene and higher OsSUT3 expression in rice panicles by qRT-PCR and transgenic research. Statistically significant results (P < 0.01) were found for this 31-bp insertions/deletions (indels) in the rice pollen development and other panicle traits, such as the pollen number, pollen fertility, seeding rate, and grain length. An evolution analysis showed that the proportion of the 31-bp insertion significantly...

Disease resistance of improved MR220 lines against Pyricularia oryzae Cavara and their preliminary agronomic performanceOriginal Paper

Siti Nor Aziemah Mohamad, Mohamad Bahagia Ab Ghaffar, Ahmad Sofiman Othman, Siti Norsuha Misman, Zuraida Abd Rahman, Mohd Shahril Firdaus Ab Razak, Zulkifli Ahmad Seman, Muhammad Fairuz Mohd Yusof, Khairulmazmi Ahmad, Siti Norhidaya Yazid, Habibuddin Hashim

Czech J. Genet. Plant Breed., 2022, 58(1):29-35 | DOI: 10.17221/55/2021-CJGPB  

Blast disease caused by Pyricularia oryzae is one of the most destructive fungal diseases of rice in Malaysia. Utilisation of resistant varieties is the most efficient management approach towards reducing yield losses. The line IRTP21683 with the Pi9 gene has shown strong resistance against the isolate MPO988.3 of pathotype P_0.0, the most prevalent P. oryzae pathotype in Malaysia. Crossing of IRTP21683 was undertaken with the recurrent parent MR220, a susceptible elite Malaysian rice variety, using a marker assisted backcrossing technique with two simple sequence repeat markers, RM19776 and RM7311, as the tag for...

Evaluation of resistance to Pseudoperonospora humuli and of the content of alpha acids and hop oils in hops of selected genetic resources of hop Humulus lupulus L.Original Paper

Markéta Trefilová, Vladimír Nesvadba, Jitka Charvátová

Czech J. Genet. Plant Breed., 2022, 58(1):36-42 | DOI: 10.17221/70/2021-CJGPB  

Twenty hop genotypes were selected for the evaluation of resistance to primary and secondary Pseudoperonospora humuli infection and of alpha acid and hop oil content in the hops. From the wild hop genotypes, two from Canada and one from Belgium showed resistance. Among the registered hop varieties, the Czech varieties Kazbek and Boomerang were the most resistant. Both wild hop genotypes from Canada showed the highest content of alpha acids among the wild hop entries, namely 4% w/w. The lowest variability of the alpha acid content in the wild hop category was found in two wild hop varieties from the Caucasus, one from Austria and one from Lithuania....