Czech Journal of Genetics and Plant Breeding - Online first
Transcriptomic profiling of shallots (Allium cepa var. aggregatum) reveals differential gene expression patterns under nitrogen deficiencyOriginal Paper
Tri Joko Santoso, Reflinur Reflinur, Zeeshan Ali, Tri Handayani, Alberta Dinar Ambarwati, Chotimatul Azmi, Atmitri Sisharmini, Aniversari Apriana, Dwinita Wikan Utami, Kristianto Nugroho
Czech J. Genet. Plant Breed., X:X | DOI: 10.17221/25/2026-CJGPB 
Nitrogen (N) is essential for crop productivity, yet excessive fertilisation decreases the nitrogen use efficiency and increases the environmental risks. Despite the economic and medicinal importance of the shallot, its molecular response to N deficiency remains unclear. We performed transcriptome profiling of two cultivars (Bima-0 and Bima 1) under N deficient and N-sufficient conditions. The RNA sequencing identified 438 differentially expressed genes (DEGs). The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed significant modulation of the nitrogen metabolism, amino acid biosynthesis, glutathione...
Quantum-Inspired Machine Learning for Screening PEG-Induced Drought Stress Responses in Caraway (Carum carvi L.)Original Paper
Samuel Goitom Misginna, Omar Gaoua, Petra Röszlerová, Musab A Isak, Ondrej Hejna, Patrick Kamulegeya, Eva Jozová, Vladislav Čurn
Czech J. Genet. Plant Breed., X:X | DOI: 10.17221/18/2026-CJGPB
Drought is a significant factor limiting the growth and early establishment of caraway (Carum carvi L.), a valuable medicinal and aromatic plant. In this study, polyethylene glycol (PEG-6000)-induced osmotic stress assays were combined with statistical and machine learning (ML) approaches to assess early drought responses in five caraway cultivars and breeding materials. Seeds were subjected to four PEG concentrations (0, 5, 10 and 15 %), and key germination and seedling traits, including germination percentage (GP), root length (RL), root fresh weight (RFW), root dry weight (RDW), shoot height (SH), shoot fresh weight (SFW), and shoot dry weight...
Assessment of genetic diversity and population structure of almond germplasm in Van Province, Türkiye, using iPBS-retrotransposon-based markersOriginal Paper
Berna Sarman, Metin Koçak
Czech J. Genet. Plant Breed., X:X | DOI: 10.17221/12/2026-CJGPB
The genetic characterisation of naturally occurring almond genotypes can guide the selection of genetic resources to be used in the breeding programme. Therefore, this study aims to assess the genetic diversity and population structure of almond germplasm comprising 50 accessions naturally occurring in Van, Türkiye, along with two commercial varieties (Pabuç and Dokuzoğuz). Thirteen inter-primer binding site (iPBS) retrotransposon markers generated a total of 102 bands, of which 95 were polymorphic. The average polymorphic band number per marker was 7.3, with a range of 5 to 13. A formula yielding a maximum of 0.5 resulted in polymorphic information...
Molecular markers and genomic resources in caraway (Carum carvi L.): Current status, research gaps, and strategic directions for breedingReview
Samuel Goitom Misginna, Petra Röszlerová, Omar Gaoua, Patrick Kamulegeya, Marie Pichová, Eva Jozová, Vladislav Čurn
Czech J. Genet. Plant Breed., X:X | DOI: 10.17221/16/2026-CJGPB
Caraway (Carum carvi L.) is an economically important spice and medicinal crop valued for its essential oil composition, particularly its high content of carvone and limonene. Despite its commercial relevance, compared with that of other Apiaceae species, the development of genomic resources remains limited. Molecular research has progressed from early dominant marker systems, including random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR), to more recent SNP-based genotyping approaches that clarify population structure and flowering-type differentiation. However, key genomic resources, such as simple sequence repeat...