Czech Journal of Genetics and Plant Breeding - Online first
Genetic characterisation of a novel male sterile two-type line system 19F08AB in Brassica napus L.Original Paper
Lirong Zhao, Zikang Chen, Ruting Xie, Hui Dong, Haibo Yu, Dongsuo Zhang, Zhaoxin Hu, Shengwu Hu
Czech J. Genet. Plant Breed., X:X | DOI: 10.17221/47/2025-CJGPB 
Rapeseed (Brassica napus L.) is a major global oilseed crop and exhibits significant heterosis. The discovery and characterisation of novel male-sterile accessions remain fundamental for harnessing heterosis in rapeseed breeding. Previously, we developed a male sterile two-type line system 19F08AB in B. napus. In this study, anther abortion in 19F08A was characterised using the squash method. The inheritance of male sterility in 19F08A and its genetic relationship to reported male sterile accessions in rapeseed was investigated using classical genetic analysis and male-sterility-gene-specific molecular markers. Results indicated that...
Evaluation of genetic characteristics and physicochemical property of Korean wheat landraces (Triticum aestivum L.)Original Paper
Yumi Lee, Sejin Oh, Seong-Wook Kang, Jaeyoung Ock, Gitak Ryu, Seul Lee, Jinhee Park, Jin-Young Moon, Jin-Young Kim, Jongtae Lee, Seong-Woo Cho
Czech J. Genet. Plant Breed., X:X | DOI: 10.17221/48/2025-CJGPB
This study has evaluated the genetic characteristics and wheat processing-related properties of four Korean wheat landraces (KWLs). The KWLs were found to possess the vernalization alleles vrn-A1, vrn-B1, and Vrn-D1 and the photoperiod alleles Ppd-A1b, Ppd-B1b, and Ppd-D1a. The Korean cultivated variety Keumgang also shared these alleles with the exception of vrn-D1. With regard to grain hardness, KWL 2 was shown to possess Pina-D1a and Pinb-D1b like Keumgang, while other KWLs were classified as carrying Pina-D1a and Pinb-D1a. All KWLs were found to be non-waxy, carrying the...
Expression and analysis of StNR and StNiRs, key enzyme genes of nitrogen assimilation in potato (Solanum tuberosum L.) with different nitrogen efficienciesOriginal Paper
Yuzhu Han, Zhijun Han, Yue Lu, Zhongcai Han, Jiayue Zhang, Jingying Zhang, Hongyu Qiao, Hongxia He
Czech J. Genet. Plant Breed., X:X | DOI: 10.17221/22/2025-CJGPB
The potato is a pivotal food crop on a global scale. Nitrate reductase (NR) and nitrite reductase (NiR) are the key enzymes in nitrogen assimilation. In previous research, we found that the nitrogen assimilation process was effectively regulated by StNR and StNiRs in potato and that there were significant differences in nitrogen utilisation efficiency between different potato varieties. In this study, three potato variants with different nitrogen use efficiency (NUE) were subjected to various nitrogen supply levels and photoperiod treatments. The results indicated that the relative expression levels of StNR and StNiRs in their leaves...
Mechanisms of overwintering frost tolerance in alfalfa roots based on 4D-Label-free quantitative proteomicsOriginal Paper
Yajun Ma, Yu Zhang, Qiaoxian Zhang, Guo Sun, Yajun Yan, Chuan Wang, Wenbin Mian, Tianhui Yang, Ting Gao
Czech J. Genet. Plant Breed., X:X | DOI: 10.17221/27/2025-CJGPB
The present study investigates the differential protein expression levels between the frost-tolerant variety Qingda No.1 and the frost-sensitive variety Gannong No.9. The analysis was conducted using 4D-Label-free quantitative proteomics technology, with the samples collected prior to and after overwintering. The results showed that the protein expression of Qingda No.1 changed more significantly during the overwintering process, with 451 differentially expressed proteins (DEPs) being identified, of which 224 were up-regulated and 227 down-regulated. In contrast, the protein expression of Gannong No.9 differed from that of alfalfa roots of the...