Czech J. Genet. Plant Breed., 2024, 60(2):79-85 | DOI: 10.17221/84/2023-CJGPB
Impact of selected antimitotic substances on doubled haploid and polyploid regeneration in microspore cultures of swede (Brassica napus ssp. napobrassica (L.) Hanelt)Original Paper
- 1 Crop Research Institute, Prague-Ruzyně, Czech Republic
- 2 Centre of the Region Haná for Biotechnological and Agricultural Research, Crop Research Institute, Olomouc, Czech Republic
- 3 Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Prague, Czech Republic
Several antimitotic agents were tested in three embryogenic doubled haploid (DH) lines of swede (Brassica napus ssp. napobrassica). No effect on embryogenesis was observed at the given concentrations of colchicine (5 μmol/L or 50 mg/L) and treatment time of 24 hours. Flow cytometric analysis of microspore embryos revealed a significant increase in the percentage of DH compared to the control for all substances: ethalfluralin, 62.2%; trifluralin, 58.3%; colchicine, 56.1%; amiprophos-methyl, 54.4%; pronamide, 35.0%; control, 13.3%. The occurrence of pure tetraploids was low (0–5%), mixoploids – especially n+2n – were high in all treatments, including the control (29.4–71.1%). Their presence can be explained as a consequence of spontaneous diploidisation and/or endoreduplication during embryogenesis or embryo germination.
Keywords: endopolyploidy; haploids; in vitro diploidization; microspore embryos; mixoploids
Received: October 10, 2023; Revised: November 15, 2023; Accepted: November 30, 2023; Prepublished online: January 3, 2024; Published: March 27, 2024 Show citation
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