Czech Journal of Genetics and Plant Breeding, 2023 (vol. 59), issue 2

Genetic diversity of some autochthonous white grape varieties from Romanian germplasm collectionsOriginal Paper

Anamaria Mirabela Ilina Dumitru, Andreea Elena Manolescu, Dorin Ioan Sumedrea, Carmen Florentina Popescu, Sina Cosmulescu

Czech J. Genet. Plant Breed., 2023, 59(2):55-66 | DOI: 10.17221/45/2022-CJGPB  

We investigated the phenotypic and genetic diversity among 25 grapevine accessions of white grape maintained in a germplasm collection ex situ. The selected varieties were characterised using standardised ampelographic descriptors and microsatellite markers to obtaintheir complete description. The ampelographic analysis based on 24 OIV descriptors and the obtained first dendrogram based on common features, revealed the extent of the phenotypic diversity ranging from 0.29 (between Coarnă albă and Chardonnay) to 0.92 (between Crâmpoşie and Cioinic), without any connection with their supposed origin or the current geographic area of distribution. The...

Gene effects for begomovirus resistance and plant architecture attributes in pumpkin (Cucurbita moschata Duchesne)Original Paper

Neha Verma, Karmvir Singh Garcha, Madhu Sharma, Abhishek Sharma, Ajmer Singh Dhatt

Czech J. Genet. Plant Breed., 2023, 59(2):67-75 | DOI: 10.17221/56/2022-CJGPB  

Knowledge of gene actions governing begomovirus resistance and plant architectural traits is a prerequisite for a successful hybrid breeding programme. Therefore, the gene actions associated with these traits were studied in two intervarietal crosses of Cucurbita moschata (C₁: Punjab Nawab × MVSR-6711 and C₂: Punjab Nawab × P-135). We used the generation mean analysis of six generations for this purpose. Significant differences between the generation means were observed for all the traits in both crosses. The parental lines differed significantly in most of the studied traits. The nature and magnitude of the gene...

Molecular mechanism of drought stress tolerance in barley (Hordeum vulgare L.) via a combined analysis of the transcriptome dataOriginal Paper

Mostafa Alamholo, Alireza Tarinejad

Czech J. Genet. Plant Breed., 2023, 59(2):76-94 | DOI: 10.17221/69/2022-CJGPB  

One of the main issues addressed by phytology in recent years has been plant tolerance mechanisms for abiotic stress. No combined analysis has been made to identify the genes involved in drought stress tolerance. The meta-analysis of microarray data related to drought stress was analysed by the R software packages and showed 3 029 upregulated genes and 3 017 downregulated genes. The upregulated genes were mostly related to the drought tolerance protein, abiotic stress response, and the Cys2His2 Zinc Finger Transcription Factor (C2H2 zinc finger TF). The downregulated genes were mainly related to the late embryogenesis abundant protein, abiotic...

QTL localisation of seed-related traits in Tibetan hulless barley based on a high-density single-nucleotide polymorphism genetic mapOriginal Paper

Yan Wang, Xiaohua Yao, Youhua Yao, Yixiong Bai, Likun An, Xin Li, Yongmei Cui, Kunlun Wu

Czech J. Genet. Plant Breed., 2023, 59(2):95-108 | DOI: 10.17221/74/2022-CJGPB  

The effective use of high-quality and high-yielding germplasm resources is of great importance for the development of hulless barley varieties. Therefore, the search for such resources has long been a goal of the breeding community. In this study, a genotyping-by-sequencing (GBS) analysis was performed on an F₂ (Nierumuzha × Kunlun 10) population. A high-density genetic map of hulless barley was constructed, which contained 1 475 efficient single-nucleotide polymorphism markers with 7 052 bin markers. The total length of the seven chromosomes was 1 139.4 cM, with an average marker distance of 0.59 cM. Based on this high-density linkage map,...

Delineation of inbred lines of Indian mustard into diverse gene pools based on agro-morphological traitsOriginal Paper

Kunwar Harendra Singh, Guman Singh, Lal Singh, Nashra Aftab, Ajay K. Thakur

Czech J. Genet. Plant Breed., 2023, 59(2):109-116 | DOI: 10.17221/75/2022-CJGPB  

The present study was conducted to explore the variability generated through recombination breeding for nine economically important traits in Indian mustard (Brassica juncea L. Czern & Coss.). Delineation of the inbred lines to different gene pools, based on genetic diversity, enables their utilisation in hybrid breeding. The unweighted pair group method with arithmetic mean (UPGMA) and a Euclidean distance matrix was used to delineate the inbred lines to clusters. The variability was studied using the range and coefficients of variation of the traits. Significant variability was observed for all studied traits except for oil content and...

List of Field Crop Varieties Registered in the Czech Republic in 2022New Varieties

Tomáš Mezlík

Czech J. Genet. Plant Breed., 2023, 59(2):117-118 | DOI: 10.17221/22/2023-CJGPB