Czech Journal of Genetics and Plant Breeding, 2024 (vol. 60), issue 1

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Editorial Department

Czech J. Genet. Plant Breed., 2024, 60(1)  

Genotypic and environmental variability and stability of seed yield, oil content and fatty acids in high-oleic and high-linoleic safflower (Carthamus tinctorius L.) lines and cultivarsOriginal Paper

Sabri Erbaş, Hasan Baydar, Halil Hatipoğlu, Hasan Koç, Metin Babaoğlu, Arzu Köse

Czech J. Genet. Plant Breed., 2024, 60(1):1-11 | DOI: 10.17221/113/2022-CJGPB  

The present study was to determine the genotypic and environmental variability and stability in seed yield, oil content, oil yield, oleic and linoleic acid of 10 safflower lines derived from a cross of Dinçer 5-18-1 × Montola 2000 together with six cultivars under six environments at five locations. The effects of genotypes, environments and genotype × environment interactions were highly significant (P < 0.01) for seed yield and oil content. Averaged across all environments, the seed yield was lowest in the cultivar Olas (2 352 kg/ha), and highest in the line Bay-Er 5 (2 869 kg/ha). According to mean (x_i) and regression...

Genetic diversity among coloured cotton genotypes in relation to their fibre colour and ploidy level based on SSR markersOriginal Paper

Revanasiddayya, Jayaprakash Mohan Nidagundi, Bashasab Fakrudin, Prakash Kuchanur, Lingappa Neelagiri Yogeesh, Shivanand Hanchinal, Talagunda Chandrashekar Suma, Gururaj Sunkad, Bharamappanavara Muralidhara, Doddamani Maheshkumar, Channabasava, Patil Sudha, Shivamurthy Rashmi, Virupapura Cholaraya Raghavendra

Czech J. Genet. Plant Breed., 2024, 60(1):12-24 | DOI: 10.17221/12/2023-CJGPB  

Genetic diversity is referred to as any variation at the phenotypic, DNA or genomic level of an individual, population or species. The appraisal of diversity is important to understand its pattern and evolutionary relationships between germplasms or genotypes, which will aid in sampling the genetic resources in a more systematic manner for conservation and crop improvement. The present study employed 50 simple sequence repeat (SSR) markers linked to the yield and fibre quality/colour traits for estimating the genetic diversity in 33 cotton genotypes of diploid and tetraploid species differing in fibre colour. The diversity analysis was performed in GenAlEx...

Development of PCR-based markers associated with powdery mildew resistance using bulked segregant analysis  (BSA-seq) in melonOriginal Paper

Yawo Mawunyo Nevame Adedze, Xia Lu, Wenyi Fan, Wenting Zhang, Xue Yang, Zhijun Deng, Md. Amirul Alam, Guangli Xu, Lihua Zhang, Wenhu Li

Czech J. Genet. Plant Breed., 2024, 60(1):25-33 | DOI: 10.17221/40/2023-CJGPB  

Powdery mildew (PM) is a fungus that causes disease in both the field and the greenhouse. Utilizing resistant cultivars is the most effective approach of disease management. To develop insertion-deletion (InDel) markers associated to this trait, the whole genomes of the PM resistant line M17050 (P1) and the PM-susceptible line 28-1-1 (P2) were sequenced. A total of 1 200 InDels, with an average of 100 markers per chromosome, were arbitrarily chosen from the sequencing data for experimental validation. One hundred InDel markers were ultimately selected due to their informative genetic bands. Further, an F₂ segregating population of melons...

Verification of resistance loci pyramiding in popular interspecific grape varieties using SSR markersOriginal Paper

Martin Hádlík, Miroslav Baránek, Ivana Flajšingerová, Kateřina Baránková

Czech J. Genet. Plant Breed., 2024, 60(1):34-41 | DOI: 10.17221/43/2023-CJGPB  

Fungal pathogens Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni (downy mildew) and Erysiphe necator Schwein. (powdery mildew) represent the biggest threats for grape growers worldwide. Under suitable conditions, these pathogens can spread very quickly through vineyards and cause significant damage. The most ecological way to reduce the possibility of infection in vineyards is growing interspecific genotypes which are able to suppress these pathogens and stop the spread of infection. With this in mind, 9 international and 11 Czech grapevine genotypes were analysed in order to genotype the resistance loci present in them....

Genomic SSR marker development in lentil (Lens culinaris Medik.) and assessment of cross-species/genera transferability to related legumesOriginal Paper

Melike Bakır, Şehriban Demir, Cebrail Yıldırım, Abdullah Kahraman

Czech J. Genet. Plant Breed., 2024, 60(1):42-49 | DOI: 10.17221/57/2023-CJGPB  

Simple sequence repeat (SSR) markers are valuable genetic and genomic research tools and are extensively used in major crops. However, a paucity of available molecular markers in lentils (Lens culinaris Medik.) has significantly hindered genomic studies in this vital legume crop. In this study, we developed 33 new SSR markers for lentils using an enriched genomic library and tested their polymorphism in 10 lentil cultivars. We found that 16 (48.5%) SSR markers were polymorphic. The expected heterozygosity values of the polymorphic SSR markers ranged from 0.095 to 0.820, while observed heterozygosity values varied between 0.100 and 1.00. In addition,...

Induction of direct somatic embryogenesis and genetic stability of somatic embryo-derived plants of broccoliShort Communication

Suzana Pavlović, Jelena Damnjanović, Zdenka Girek, Lela Belić, Milan Ugrinović

Czech J. Genet. Plant Breed., 2024, 60(1):50-54 | DOI: 10.17221/26/2023-CJGPB  

The influence of the developmental stage of zygotic embryos and the composition and pH of the Gamborg induction medium B5 on the initiation and development of somatic embryos was investigated. The optimal medium was B5 medium with a pH value of 5.0 and without plant growth regulator, at which the highest frequency of somatic embryogenesis (56.67%) and the highest average number of somatic embryos per explant (3.35) were achieved. Somatic embryos appeared directly on the hypocotyls of the explants, without the callus stage. On zygotic embryos in the early cotyledonary phase, three times higher regeneration was achieved compared to larger embryos in the...