Czech J. Genet. Plant Breed., 2024, 60(2):70-78 | DOI: 10.17221/72/2023-CJGPB

Malting quality molecular markers for barley breedingOriginal Paper

Leona Leišová-Svobodová1*, Vratislav Psota2, Marta Zavřelová3, Martin Kříž4, Pavel Mařík4, Zdeněk Nesvadba1
1 Crop Research Institute, Prague Ruzyně, Czech Republic
2 Research Institute of Brewing and Malting, Brno, Czech Republic
3 Agrotest fyto, s.r.o., Kroměříž, Czech Republic
4 SELGEN, a.s., Sibřina, Czech Republic

Barley is one of the world’s most important crops. Barley is used for both food and feed and is important for the production of malt. Malt quality is a complex function of barley genetics, environmental conditions during barley growth, and the technological aspects of the malting process. Due to the high heritability of more than half of the malting parameters, barley can be bred for malting quality. Marker-assisted selection (MAS) is a good way to speed up the breeding process. In this study, nine molecular markers were used to screen 115 barley varieties and breeding lines over a four-year period. The results were compared with malting quality parameters. Multicomponent correlation analysis showed a good correlation (R = 0.63; P ≤ 0.01) between marker screening results and malting quality parameters. In 93 genotypes (80.9%), agreement was found between molecular marker prediction and malting quality determination. Differences between molecular marker screening and malt quality parameters and possible improvements are discussed. The use of molecular markers in MAS is highly appreciated by barley breeders.

Keywords: Hordeum vulgare L.; malting quality parameters; marker-assisted selection; polymorphism

Received: August 29, 2023; Revised: November 1, 2023; Accepted: November 2, 2023; Prepublished online: January 2, 2024; Published: March 27, 2024  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Leišová-Svobodová L, Psota V, Zavřelová M, Kříž M, Mařík P, Nesvadba Z. Malting quality molecular markers for barley breeding. Czech J. Genet. Plant Breed. 2024;60(2):70-78. doi: 10.17221/72/2023-CJGPB.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files:

    Download file72-2023_CJGPB_ESM.xlsx

    File size: 59.22 kB

    References

    1. American Society of Brewing Chemists (2011): ASBC Methods of Analysis Sprout Damage by the Falling Number Method (Barley 12A). 14th Ed. St. Paul, American Society of Brewing Chemists.
    2. Aubert M.K., Coventry S., Shirley N.J., Bettes N.S., Würschum T., Burton R.A., Tucker M.R. (2018): Differences in hydrolytic enzyme activity accompany natural variation in mature aleurone morphology in barley (Hordeum vulgare L.). Scientific Reports, 8: 11025. Go to original source... Go to PubMed...
    3. Collins H.M., Panozzo J.F., Logue S.J., Jefferies S.P., Barr A.R. (2003): Mapping and validation of chromosome region associated with high malt extract in barley (Hordeum vulgare L.). Australian Journal of Agricultural Research, 54: 1223-1240. Go to original source...
    4. Duan R., Xiong H., Wang A., Chen G. (2015): Molecular mechanisms underlying hull-caryopsis adhesion/separation revealed by comparative transcriptomic analysis of covered/naked barley (Hordeum vulgare L.). Integrative Journal of Molecular Sciences, 16: 14181-14193. Go to original source... Go to PubMed...
    5. EBC Analysis Committee (2010): Analytica EBC. The European Reference Methods for Breweries by European Brewery Convention. Nurenberg, Fachverlag Hans Carl.
    6. Fechter I., Rath F., Voetz M. (2010): A single PCR marker predicting the activity levels of various enzymes responsible for malting quality. Journal of the American Society of Brewing Chemists, 68: 41-47. Go to original source...
    7. Fox G.P., Panozzo J.F., Li C.D., Lance R.C.M., Inkerman P.A., Henry R.J. (2003): Molecular basis of barley quality. Australian Journal of Agricultural Research, 54: 1081-1101. Go to original source...
    8. Genievskaya Y., Almerekova S., Abugalieva S., Chudinov V., Blake T., Abugalieva A., Turuspekov Y. (2022): Identification of SNP markers associated with grain quality traits in a barley collection (Hordeum vulgare L.) harvested in Kazakhstan. Agronomy, 12: 2431. Go to original source...
    9. Gong X., Westcott S., Zhang X.Q., Yan G., Lance R., Zhang G., Sun D., Li C. (2013): Discovery of novel Bmy1 alleles increasing β-amylase activity in Chinese landraces and Tibetan wild barley for improvement of malting quality via MAS. PLoS ONE, 8: e72875. Go to original source... Go to PubMed...
    10. Han F., Romagosa I., Ulrich S.E., Jones B.L., Hayes P.M., Wesenberg D.M. (1997): Molecular marker-assisted selection for malting quality traits in barley. Molecular Breeding, 3: 427-437. Go to original source...
    11. Hotelling H. (1936): Relations between two sets of variates. Biometrika, 28: 321-377. Go to original source...
    12. Igartua E., Edney M., Rossnagel B.G., Spaner D., Legge W.G., Scoles G.J., Eckstein G.A., Penner G.A., Tinker N.A., Briggs K.G., Falk D.E., Mather D.E. (2000): Marker-based selection of QTL affecting grain and malt quality in two-row barley. Crop Science, 40: 1426-1433. Go to original source...
    13. Iqbal I., Abera Desta A., Tripathi R.J., Beattie A., Ba-dea A., Singh J. (2023): Interaction and association analysis of malting related traits in barley. PLoS ONE, 18: e0283763. Go to original source... Go to PubMed...
    14. Kochevenko A., Jiang Y., Seiler Ch., Surdonja K., Kollers S., Reif J.Ch., Korzun V., Graner A. (2018): Identification of QTL hot spots for malting quality in two elite breeding lines with distinct tolerance to abiotic stress. BMC Plant Biology, 18: 106. Go to original source... Go to PubMed...
    15. Leišová-Svobodová L., Psota V., Stočes Š., Vácha P., Ku-čera L. (2020): Comparative de novo transcriptome analysis of barley varieties with different malting qualities. Functional and Integrative Genomics, 20: 801-812. Go to original source... Go to PubMed...
    16. MEBAK (2011): Raw Materials: Barley, Adjuncts, Malt, Hops and Hop Products. Collection of Beringu Analysis Methods of the Mitteleuropäische Brautechnische Analysenkommission (MEBAK). Freising-Weihenstephan, MEBAK.
    17. Meloun M., Militký J., Hill M. (2005): Computer Analysis of Multivariate Data in Examples. Prague, Academia: 94-136. (in Czech)
    18. Molina-Cano J.L., Francesch M., Perez A.M., Ramo T., Voltas J., Brufau J. (1997): Genetic and environmental variation in malting and feed quality of barley. Journal of Cereal Science 25: 37-47. Go to original source...
    19. Perrier X., Flori A., Bonnot F. (2003): Data analysis methods. In: Hamon P., Seguin M., Perrier X., Glaszmann J.C. (eds.): Genetic Diversity of Cultivated Tropical Plants. Montpellier, Enfield Science Publishers: 43-76.
    20. Perrier X., Jacquemoud-Collet J.P. (2006): DARwin software. Available at http://darwin.cirad.fr/ darwin.
    21. Pritchard J.K., Stephens M., Donnelly P. (2000): Inference of population structure from multilocus genotype data. Genetics, 155: 945-959. Go to original source... Go to PubMed...
    22. Saghai-Maroof M.A., Soliman K.M., Jorgensen R.A., Allard R.W. (1984): Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal locations, and population dynamics. Proceedings of the National Academy of Sciences of the USA, 81: 8014-8018. Go to original source... Go to PubMed...
    23. Trubascheeva N.V., Pershina L.A. (2021): Problems and possibilities of studying malting quality in barley using molecular genetic approaches. Vavilov Journal of Genetics and Breeding, 25: 171-177. Go to original source... Go to PubMed...
    24. Zhang W.S., Li X., Liu J.B. (2007): Genetic variation of Bmy1 alleles in barley (Hordeum vulgare L.) investigated by CAPS analysis. Theoretical and Applied Genetics, 114: 1039-1050. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content