Czech Journal of Genetics and Plant Breeding - Latest articles
Results 61 to 68 of 68:
Molecular mechanism of drought stress tolerance in barley (Hordeum vulgare L.) via a combined analysis of the transcriptome data
Mostafa Alamholo, Alireza Tarinejad
Czech J. Genet. Plant Breed., 2023, 59(2):76-94 | DOI: 10.17221/69/2022-CJGPB 
One of the main issues addressed by phytology in recent years has been plant tolerance mechanisms for abiotic stress. No combined analysis has been made to identify the genes involved in drought stress tolerance. The meta-analysis of microarray data related to drought stress was analysed by the R software packages and showed 3 029 upregulated genes and 3 017 downregulated genes. The upregulated genes were mostly related to the drought tolerance protein, abiotic stress response, and the Cys2His2 Zinc Finger Transcription Factor (C2H2 zinc finger TF). The downregulated genes were mainly related to the late embryogenesis abundant protein, abiotic...
Gene effects for begomovirus resistance and plant architecture attributes in pumpkin (Cucurbita moschata Duchesne)
Neha Verma, Karmvir Singh Garcha, Madhu Sharma, Abhishek Sharma, Ajmer Singh Dhatt
Czech J. Genet. Plant Breed., 2023, 59(2):67-75 | DOI: 10.17221/56/2022-CJGPB
Knowledge of gene actions governing begomovirus resistance and plant architectural traits is a prerequisite for a successful hybrid breeding programme. Therefore, the gene actions associated with these traits were studied in two intervarietal crosses of Cucurbita moschata (C1: Punjab Nawab × MVSR-6711 and C2: Punjab Nawab × P-135). We used the generation mean analysis of six generations for this purpose. Significant differences between the generation means were observed for all the traits in both crosses. The parental lines differed significantly in most of the studied traits. The nature and magnitude of the gene...
Genetic diversity of some autochthonous white grape varieties from Romanian germplasm collections
Anamaria Mirabela Ilina Dumitru, Andreea Elena Manolescu, Dorin Ioan Sumedrea, Carmen Florentina Popescu, Sina Cosmulescu
Czech J. Genet. Plant Breed., 2023, 59(2):55-66 | DOI: 10.17221/45/2022-CJGPB
We investigated the phenotypic and genetic diversity among 25 grapevine accessions of white grape maintained in a germplasm collection ex situ. The selected varieties were characterised using standardised ampelographic descriptors and microsatellite markers to obtaintheir complete description. The ampelographic analysis based on 24 OIV descriptors and the obtained first dendrogram based on common features, revealed the extent of the phenotypic diversity ranging from 0.29 (between Coarnã albã and Chardonnay) to 0.92 (between Crâmpoºie and Cioinic), without any connection with their supposed origin or the current geographic area of distribution. The...
Further fine mapping and candidate gene prediction for a new restoring fertility gene Rf(fa) in rice
Yu Li, Xiaomi Chen, Tao Lan, Jing Zhang, Ziheng Chen, Wenting Yang, Xinmei Lin
Czech J. Genet. Plant Breed., 2023, 59(1):43-53 | DOI: 10.17221/49/2022-CJGPB
Rf(fa), a new restoring fertility gene in rice, was previously located to a large region on Chromosome 10. The large number of genes within the region made cloning of Rf(fa) difficult. To perform the cloning and further elucidate the molecular mechanism, we reconstructed a mapping segregation population (BC1F1) of 12 000 plants. Using the population and polymorphism of simple sequence repeat (SSR) molecular markers, we finally mapped Rf(fa) between the two SSR molecular markers MM2000 and RM25658, within a 78.87 kb region. By de novo sequencing of a restoring line of CMS-FA hybrid rice, we obtained...
Transcriptome and proteome analysis of the fig (Ficus carica L.) cultivar Orphan and its mutant Hongyan based on the fruit peel colour in South China
Lingzhu Wei, Jianhui Cheng, Jiang Xiang, Ting Zheng, Jiang Wu
Czech J. Genet. Plant Breed., 2023, 59(1):33-42 | DOI: 10.17221/42/2022-CJGPB
The external fruit colour is an important parameter of the fig fruit quality. Fig anthocyanin content is critical for the peel colour. The peel of mature fruits of the fig cultivar Orphan and its red peel bud mutant Hongyan were separated for a transcriptomic and proteomic analysis. A total of 162 different abundance proteins (DAPs) and 5 015 differentially expressed genes (DEGs) were identified. The correlation analysis revealed that only two and 15 genes were downregulated and upregulated, respectively, at both the transcriptome and proteome levels. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that the enrichment pathways...
Genetic variability for resistance to fungal pathogens in bread wheat
Mian Abdur Rehman Arif, Edward Arseniuk, Andreas Börner
Czech J. Genet. Plant Breed., 2023, 59(1):23-32
Sustainable global wheat production requires wheat varieties, that are sufficiently resistant to the main wheat diseases. The economically important fungal pathogens worldwide include powdery mildew (PM), yellow rust (YR), leaf rust (LR) and blotch causing pathogens including Septoria nodorum blotch (SNB) and Septoria tritici blotch (STB). Here, we present the evaluation of winter wheat varieties of diverse origin against the prevalent local populations of PM, YR, LR, STB and SNB under natural infection conditions through image-based phenotyping in two consecutive years (2019 and 2020). We found several varieties to be resistant against multiple diseases....
Genetic diversity and proteomic analysis of vegetable soybean (Glycine max (L.) Merrill) accessions grown in mineral and BRIS soils
Nor Hafizah Zakaria, Mohd Shukor Nordin, Maizatul Akma Ibrahim, Fadzilah Adibah Abdul Majid, Zarina Zainuddin
Czech J. Genet. Plant Breed., 2023, 59(1):14-22 | DOI: 10.17221/38/2022-CJGPB
Knowledge of the molecular mechanisms of response to environmental stress is fundamental for the development of genetically stress-tolerant crops. This study aims to find vegetable soybean accessions tolerant to cultivation in stressful tropical environments. Fourteen accessions of the vegetable soybean (Glycine max (L.) Merrill) were grown in mineral and beach ridges interspersed with swale (BRIS) soils. The genetic diversity, estimated using inter-simple sequence repeat (ISSR) markers, revealed 42.50% polymorphism and was regarded as moderate. The unweighted pair-group method arithmetic average (UPGMA) analysis allocated the tested accessions...
Diverse role of basic Helix-Loop-Helix (bHLH) transcription factor superfamily genes in the fleshy fruit-bearing plant species
Noor Muhammad, Nisar Uddin, Muhammad Khalil Ullah Khan, Niaz Ali, Kishwar Ali, David Aaron Jones
Czech J. Genet. Plant Breed., 2023, 59(1):1-13
The basic Helix-Loop-Helix (bHLH) superfamily is the most widespread family of transcription factors in eukaryotic organisms, which can activate the expression of genes by interacting with specific promoters in the genes. The bHLH transcription factors direct the development and metabolic process of plants, including flowering initiation and secondary metabolite production, by attaching to specific sites on their promoters. These transcription factors are essential for encouraging plant tolerance or the adjustment to harsh environmental conditions. The involvement of bHLH genes in anthocyanin formation in fleshy fruit-bearing plants, as well...