Czech J. Genet. Plant Breed., 2023, 59(4):219-234 | DOI: 10.17221/9/2023-CJGPB

Identification of genes for leaf rust resistance in seedlings of wheat cultivars from the Yellow-Huai Basin in China and slow rusting observations in field trialsOriginal Paper

Huali Yang1, Wenda Diao1, Xiaocui Yan1, Takele-Weldu Gebrewahid2, Zaifeng Li3*, Zhanjun Yao1*
1 College of Agronomy, Hebei Agricultural University, North China Key Laboratory for Crop Germplasm Resources of China’s Education Ministry, Baoding, Hebei, P.R. China
2 College of Agriculture, Aksum University, Shire-Indaslassie, Tigray, Ethiopia
3 College of Plant Protection, Agricultural University of Hebei/Biological Control Center for Plant Disease and Pests of Hebei Province, Baoding, Hebei, P.R. China

Wheat leaf rust is a devastating disease worldwide. Identification of leaf rust resistance genes in seedlings and of genes for slow rusting are important in resistance breeding and for gene deployment to control the disease. A total of 108 wheat cultivars from the Beijing and Shandong province and a set of 36 differentials, mostly near-isogenic lines in the background of Thatcher with known leaf rust resistance genes, were tested with 20 Puccinia triticina pathotypes  (FHJS1, FGBQ,PGJQ, SHJT, FHGQ, PHTT1, FHGQ, FHGQ, PHJS, THSM, FHSQ, PHST, PRSQ, FNTQ, PHGM, KHGQ, PHTT2, TGTT, FHJS2, NHHT) at the seedling stage in the greenhouse. The cultivars and differentials were also planted in the field to test their slow rusting resistance using a mixture of races at Baoding, Hebei province and Zhoukou, Henan province, for two consecutive years. Ten leaf rust resistance genes, Lr1, 9, 10, 19, 20, 24, 26, 34, 37 and 46 were identified  in the 57 commercial wheat cultivars, either singly or in combination, using molecular markers. Combined, the results from gene postulation and marker detection showed that one or more of the genes Lr1, 3, 10, 14a, 14b, 26, 36, 39, 34 and 46, were present in 57 cultivars, and that no known resistance gene was present in the remaining 51 cultivars. The resistance gene Lr26 was present in 42 cultivars, and nine cultivars contained Lr1. Lr46 was present in 10 cultivars, as indicated by the presence of the closely linked marker csLV46G22. Seven genotypes were identified as possibly carrying the gene Lr39. Lr3 and 10 were found in six and four genotypes, respectively. The ggenes Lr14b and 34 were each present in three cultivars, while evidence for the presence of Lr14a and 36 was obtained in single genotypes. Finally, 12 cultivars showed slow rusting resistance at two locations in two crop seasons. The identification of leaf rust resistance genes in Chinese wheat cultivars will be helpful for gene deployment to control leaf rust.

Keywords: gene postulation; Lr genes; molecular marker; slow rusting resistance; Puccinia triticina; Triticum aestivum

Received: January 31, 2023; Revised: March 28, 2023; Accepted: March 29, 2023; Prepublished online: June 6, 2023; Published: September 11, 2023  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Yang H, Diao W, Yan X, Gebrewahid T, Li Z, Yao Z. Identification of genes for leaf rust resistance in seedlings of wheat cultivars from the Yellow-Huai Basin in China and slow rusting observations in field trials. Czech J. Genet. Plant Breed. 2023;59(4):219-234. doi: 10.17221/9/2023-CJGPB.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files:

    Download fileYang_ESM.pdf

    File size: 125.17 kB

    References

    1. Ash G. (1996): Wheat Rusts: An Atlas of Resistance Genes. Australasian Plant Pathology, 25: 70. Go to original source...
    2. Bariana H.S., McIntosh R.A. (1993): Cytogenetic studies in wheat. XV. Location of rust resistance genes in VPM1 and their genetic linkage with other disease resistance genes in chromosome 2A. Genome, 36: 476-482. Go to original source... Go to PubMed...
    3. Boroujeni F.R., Arzani A., Afshari F., Torabi M. (2011): Postulation of leaf rust resistance genes in Iranian wheat cultivars and breeding lines. Canadian Journal of Plant Pathology, 33: 550-558. Go to original source...
    4. Caldwell R.M. (1968): Breeding for general and/or specific plant disease resistance. In: K. W. Finlay K.W., Shepherd K.W. (eds.): Proc. 3rd Int. Wheat Genetics Symposium, Canberra. Sydney, Butterworths: 263-272.
    5. Chai J.F., Zhou R.H., Jia J., Liu X. (2006): Development and application of a new codominant PCR marker for detecting 1BL center dot 1RS wheat-rye chromosome translocations. Plant Breeding, 125: 302-304. Go to original source...
    6. Cherukuri D.P., Gupta S.K., Charpe A., Koul S., Prabhu K.V., Singh R.B., Haq Q.M.R. (2005): Molecular mapping of Aegilops speltoides derived leaf rust resistance gene Lr28 in wheat. Euphytica, 143:19-26. Go to original source...
    7. Cloutier S., McCallum B.D., Loutre C., Banks T.W., Wicker T., Feuillet C., Keller B., Jordan M.C. (2007): Lr gene Lr1, isolated from bread wheat (Triticum aestivum L.) is a member of the large psr567 gene family. Plant Molecular Biology, 65: 93-106. Go to original source... Go to PubMed...
    8. Dong J.G. (2001): Agricultural Plant Pathology. Beijing, China Agriculture Press.
    9. Dubin H.J., Torres E. (1981): Causes and consequences of the 1976-1977 wheat leaf rust epidemic in northwest Mexico. Annual Review of Phytopathology, 19: 41-49. Go to original source...
    10. Dubin H.J., Johnson R., Stubbs R.W. (1989): Postulated gene-for-gene resistant to stripe rust in selected CIMMYT and related wheat. Plant Disease, 73: 472-475. Go to original source...
    11. Dyck P.L. (1977): Genetics of leaf rust reactions in three introductions of common wheat. Canadian Journal of Genetics and Cytology, 19: 711-716. Go to original source...
    12. Dyck P.L. (1987): The association of a gene for leaf rust resistance with the chromosome 7D suppressor of stem rust resistance in common wheat. Genome, 29:467-469. Go to original source...
    13. Flor H.H. (1956): The complementary gene systems in flax and flax rust. Advances in Genetics, 8: 29-54. Go to original source...
    14. Froidmont D. (1998): A co-dominant marker for the 1BL/1RS wheat-rye translocation via multiplex PCR. Journal of Cereal Science, 27: 229-232. Go to original source...
    15. Gill K.S., Lubbers E.L., Gill B.S., Raupp W.J., Cox T.S. (1991): A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD). Genome, 34: 362-374. Go to original source...
    16. Gupta S.K., Charpe A., Koul S., Prabhu K.V., Haque Q.M. (2005): Development and validation of molecular markers linked to an Aegilops umbellulata-derived leaf-rust-resistance gene, Lr9, for marker-assisted selection in bread wheat. Genome, 48: 823-830. Go to original source... Go to PubMed...
    17. Gupta S.K., Charpe A., Koul S., Haque Q., Prabhu K. (2006): Development and validation of SCAR markers co-segregating with an Agropyron elongatum derived leaf rust resistance gene Lr24 in wheat. Euphytica, 150: 233-240. Go to original source...
    18. Han Y., He Z.H., Li Z.F. (2010): Seedling and slow rusting resistances to leaf rust in CIMMYT wheat lines. Acta Agronomica Sinica, 37: 1125-1133.
    19. He Z.H., Rajaram S., Xin Z.Y., Zhang G.Z. (2001): A history of wheat breeding in China. Journal of Comparative Neurology, 523: 805-813.
    20. Herrera-Foessel S.A., Lagudah E.S., Huerta-Espino J., Hayden M.J., Bariana H.S., Singh D., Singh R.P. (2011): New slow-rusting leaf rust and stripe rust resistance genes Lr67 and Yr46 in wheat are pleiotropic or closely linked. Theoretical and Applied Genetics, 122: 239-249. Go to original source... Go to PubMed...
    21. Herrera-Foessel S.A., Singh R.P., Huerta-Espino J., Rosewarne G.M., Periyannan S., Viccars L., Calvo-Salazar V., Lan C., Lagudah E.S. (2012): Lr68: A new gene conferring slow rusting resistance to leaf rust in wheat. Theoretical and Applied Genetics, 124: 1475-1486. Go to original source... Go to PubMed...
    22. Hiebert C.W., Thomas J.B., McCallum B.D., Humphreys D.G., DePauw R.M., Hayden M.J, Mago R., Schnippenkoetter W., Spielmeyer W. (2010): A new gene, Lr67, from the wheat accession PI250413 confers resistance to leaf rust at the adult plant stage. Theoretical and Applied Genetics, 121: 1083-1091. Go to original source... Go to PubMed...
    23. Khan M.H., Bukhari A., Da Z.A., Rizvi S.M. (2013): Status and strategies in breeding for rust resistance in wheat. Agricultural Sciences, 04: 292-301. Go to original source...
    24. Li Z.F., Xia X.C., He Z.H., Li X., Zhang L.J., Wang H.Y., Meng Q.F., Yang W.X., Li G.Q., Liu D.Q. (2010): Seedling and slow rusting resistance to leaf rust in Chinese wheat cultivars. The American Phytopathological Society, 94: 45-53. Go to original source... Go to PubMed...
    25. Lillemo M., Asalf B., Singh R.P., Huerta-Espino J., Chen X.M., He Z.H., Bjornstad A. (2008): The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line saar. Theoretical and Applied Genetics, 116: 1155-1166. Go to original source... Go to PubMed...
    26. Liu X.C., Lai Y.P., Feng Z.Y. (2014): Distribution and selective effects of Lr1 gene in the wheat varieties derived from four founder breeding parents in China. Journal of Triticeae Crops, 34: 597-602.
    27. Long D.L., Kolmer J.A. (1989): A North American system of nomenclature for Puccinia recondita f.sp. Tritici. Phytopathology, 79: 525-529. Go to original source...
    28. Mago R., Spielmeyer W., Lawrence G., Lagudah E., Ellis J., Pryor, A. (2002): Identification and mapping of molecular markers linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines. Theoretical and Applied Genetics, 104: 1317-1324. Go to original source... Go to PubMed...
    29. McIntosh R.A. (1992): Pre-emptive breeding to control wheat rust. Euphytica, 63: 103-113. Go to original source...
    30. McIntosh R.A., Baker E.P.(1968): In: Finlay K.W., Shepard K.W. (eds.): 3rd Int.Wheat Genetics Symposium, Canberra. Australian Academy of Science, Butterworth: 305-308.
    31. McIntosh R.A., Yamazaki Y., Devos K.M., Dubcovsky J., Rogers J., Appels R. (2003): Catalog of gene symbols for wheat. In: Pogna N.E., Romano N., Pogna E., Galterio G. (eds): Xth Int. Wheat Genetics Symposium. Roma, Instituto Sperimentale per la Cerealcoltura.
    32. McIntosh R.A., Dubcovsky J., Rogers W.J., Morris C., Appels R., Xia X.C. (2016): Catalogue of gene symbols for wheat (2015-2016) supplement. Available at https://shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement2015.pdf
    33. Neu C., Stein N., Keller B. (2002): Genetic mapping of the Lr20-Pm1 resistance locus reveals suppressed recombination on chromosome arm 7AL in hexaploid wheat. Genome. 45: 737-744. Go to original source... Go to PubMed...
    34. Park R.F., McIntosh R.A. (1994): Adult plant resistances to Puccinia recondita f.sp. tritici in wheat. New Zealand Journal of Crop and Horticultural Science, 22: 151-158. Go to original source...
    35. Park R.F., Burdon J.J., McIntosh R.A. (1995): Studies on the origin, spread, and evolution of an important group of Puccinia recondita f. sp. tritici pathotypes in Australasia. European Journal of Plant Pathology, 101: 613-622. Go to original source...
    36. Peterson R.F., Campbell A.B., Hannah A.E. (1948): A diagrammatic scale for estimating rust intensity of leaves and stem of cereals. Canadian Journal of Forest Research, 26: 496-500. Go to original source...
    37. Pink D.A.C. (2002): Strategies using genes for non-durable disease resistance. Euphytica, 124: 227-236. Go to original source...
    38. Prins R., Groenewald J.Z., Marais G.F., Snape J.W., Koebner R.M.D. (2001): AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat. Theoretical and Applied Genetics, 103: 618-624. Go to original source...
    39. Ren X.L., Liu T.G., Liu B., Gao L., Chen W.Q. (2015): Postulation of seedling leaf rust resistance genes in 84 Chinese winter wheat cultivars. Journal of Integrative Agriculture, 14: 1992-2001. Go to original source...
    40. Roelfs A.P., Singh R.P., Saari E.E. (1992): Rust diseases of wheat: Concepts and methods of diseases of wheat: Concepts and methods of disease management. CIMMYT, Mexico.
    41. Schachermayr G., Feuillet C., Keller B. (1997): Molecular markers for the detection of the wheat leaf rust resistance gene Lr10 in diverse genetic backgrounds. Molecular Breeding, 3: 65-74. Go to original source...
    42. Schnurbusch T., Bossolini E., Messmer M., Keller B. (2004): Tagging and validation of a major quantitative trait locus for leaf rust resistance and leaf tip necrosis in winter wheat cultivar Forno. Phytopathology, 94: 1036-1041. Go to original source... Go to PubMed...
    43. Singh R.P., Rajaram S. (2011). Genetics of adult plant resistance of leaf rust in 'Frontana' and three CIMMYT wheats. Genome, 35: 24-31. Go to original source...
    44. Singh R.P., Mujeeb-Kazi A., Huerta-Espino J. (1998): Lr46: a gene conferring slow rusting resistance to leaf rust to leaf rust in wheat. Phytopathology, 88: 890-894. Go to original source... Go to PubMed...
    45. Singh R.P., Chen W.Q., He Z.H. (1999): Leaf rust resistance of spring, facultative, and winter wheat cultivars from China. Plant Disease, 83: 644-651. Go to original source... Go to PubMed...
    46. Singh D., Park R.F., McIntosh R.A. (2001): Postulation of leaf (brown) rust resistance genes in 70 wheat cultivars grown in the United Kingdom. Euphytica, 120: 205-218. Go to original source...
    47. Winzeler M., Mesterhazy A., Park R.F., Bartos P., Csosz M., Goyeau H., Ittu M., Jones E., Loschenberger F., Manninger K., Pasquini M., Richter K., Rubiales D., Schachermayer G., Strze-mbicka A., Trott M., Unger O., Vida G., Walther U. (2000): Resistance of European winter wheat germplasm to leaf rust. Agronomie, 20: 783-792. Go to original source...
    48. Yan X.C., Li Z.F., Yang H.L., Yao Z.J., Liu D.Q. (2017): Analysis of wheat leaf rust resistance genes in 30 important wheat cultivars. Scientia Agricultura Sinica, 50: 272-285.
    49. Yan X.C., Gebrewahid T.W., Li X., Xu X.Y., Zhang P.P., Yao Z.J., Li Z.F. (2021): Identification of known leaf rust resistance genes in bread wheat cultivars from China. Czech Journal of Genetics and Plant Breeding, 57: 97-101. Go to original source...
    50. Yuan J.H., Liu T.G., Chen W.Q. (2007): Postulation of leaf rust resistance genes in 47 new wheat cultivars at seedling stage. Scientia Agricultura Sinica, 40: 1925-1935.
    51. Zhang L.J., Li Z.F., Lillemo M., Xia X.C., Liu D.Q., Yang W.X., Luo J.C.,Wang H.Y. (2009): QTL mapping for adult-plant resistance to leaf rust in CIMMYT wheat cultivar Saar. Scientia Agricultura Sinica, 42: 388-397.
    52. Zhou Y., Wu Y., Li X., Li Z.F., Liu D.Q. (2012): Genetic analysis and gene mapping of resistance gene in two Chinese cultivars. Scientia Agricultura Sinica, 45: 3273-3280.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content