Czech Journal of Genetics and Plant Breeding, 2019 (vol. 55), issue 3
Physiological and molecular aspects of pod shattering resistance in cropsReview
Xiaobing Liu, Bingjie Tu, Qiuying Zhang, Stephen J. Herbert
Czech J. Genet. Plant Breed., 2019, 55(3):87-92 | DOI: 10.17221/104/2018-CJGPB 
Pod shattering resistance is a trait acquired by crops in the process of evolution. Manipulation of physiological and molecular processes is fundamental for the improvement of shattering resistance in crops. In this review we discuss several enzymes, key hormones and their possible roles or relationships involved in pod shattering, and highlight responsible genes, quantitative traits loci (QTLs) and their implications for increased pod shattering resistance. Cell wall degrading enzymes, particularly β-glucanases and endopolygalacturonases play an important role in the process of pod dehiscence. It is not clear how and to what extent a specific...
Breeding of Indica glutinous cytoplasmic male sterile line WX209A via CRISPR/Cas9 mediated genomic editingOriginal Paper
Xin Wang, Yue Han, Xuan Feng, Yun-Zhen Li, Bao-Xiang Qin, Ji-Jing Luo, Zheng Wei, Yong-Fu Qiu, Fang Liu, Rong-Bai Li
Czech J. Genet. Plant Breed., 2019, 55(3):93-100 | DOI: 10.17221/197/2017-CJGPB
Glutinous cytoplasmic male sterile (CMS) line is necessary to select hybrid glutinous rice combination with high yield and quality. To develop glutinous CMS with low amylose content, in this study, we firstly knocked out the granule-bound starch synthase OsWaxy in 209B using CRISPR/Cas9 mediated genome editing technology and successfully obtained a glutinous maintainer line WX209B. Comparing with maintainer line 209B, WX209B showed decreased amylose contents and similar agronomic characters. And then, through one generation of hybridization and two generations of backcrossing with WX209B as the male parent and 209A as the female
Efficiency of three haplomethods in durum wheat (Triticum turgidum subsp. durum Desf.): isolated microspore culture, gynogenesis and wheat × maize crossesOriginal Paper
Olfa Slama-Ayed, Imen Bouhaouel, Sourour Ayed, Jacques De Buyser, Emmanuel Picard, Hajer Slim Amara
Czech J. Genet. Plant Breed., 2019, 55(3):101-109 | DOI: 10.17221/188/2017-CJGPB
This study presents the first report comparing the efficiency of microspore culture, gynogenesis and durum wheat × maize crosses for haploid plant production from three durum wheat genotypes (Razzek, Karim and Jneh Khotifa). The results showed that the best induction, calli or embryos formation and plant regeneration rates for the three genotypes were obtained with gynogenesis (47.2, 7.6, 0.8%), followed by interspecific crosses (33.1, 1.7, 0.4%) and isolated microspore culture...
Phenotyping and SSR markers as a tool for identification of duplicates in lettuce germplasmOriginal Paper
Michal Sochor, Michaela Jemelková, Ivana Doležalová
Czech J. Genet. Plant Breed., 2019, 55(3):110-119 | DOI: 10.17221/68/2018-CJGPB
In total, 117 individual samples from 39 accessions of Lactuca sativa were selected from the Czech national collection of lettuce with the aim to quantify and compare patterns of genetic and phenotypic variability within and among lettuce accessions and to propose a rapid, reliable and inexpensive method for verification of possible duplicates. We focused on phenotypic evaluation and SSR genotyping, and studied their ability to distinguish between individual accessions. Phenotypic data revealed that no two accessions shared the exactly same phenotype and no accession exhibited variability in the characters studied. Variability in SSR markers...
Effect of different factors on regeneration and transformation efficiency of tomato (Lycopersicum esculentum) hybridsOriginal Paper
Evangelia Stavridou, Nikoleta A. Τzioutziou, Panagiotis Madesis, Nikolaos E. Labrou, Irini Nianiou-Obeidat
Czech J. Genet. Plant Breed., 2019, 55(3):120-127 | DOI: 10.17221/61/2018-CJGPB
The current study aimed to produce rootstock material through micropropagation by developing efficient regeneration and Agrobacterium-mediated transformation protocols for three high quality commercial tomato hybrids (Felina, Siena and Don Jose) to overexpress the GmGSTU4 gene from Glycine max L. previously shown to enhance antioxidant activity. We investigated the plant growth regulators zeatin (Z) and 3-idoleacetic acid (IAA) to determine their best combination for an efficient regeneration protocol for each hybrid. The highest regeneration efficiency was observed in Felina (94.4%) with 1.0 mg/l Z and 0.1 mg/l IAA. In contrast,...
Spring barley variety Laudis 550Short Communication
Radim Korhoň, Petr Svačina, Dana Komoňová, Monika Škopová
Czech J. Genet. Plant Breed., 2019, 55(3):128-130 | DOI: 10.17221/41/2018-CJGPB
Laudis 550 is a mid-late malting spring barley variety, medium resistant to lodging and medium resistant to stem brackling. It is resistant to powdery mildew, medium resistant to brown rust, leaf blotch complex and scald. The variety reached 7.2 points of the malting quality index (to the registration date) and it is recommended by the Research Institute of Brewing and Malting, Plc for production of beer with the protected geographical indication České pivo (Czech beer).