Czech Journal of Genetics and Plant Breeding, 2017 (vol. 53), issue 3
Application of next-generation sequencing in plant breedingReview
David VLK, Jana ŘEPKOVÁ
Czech J. Genet. Plant Breed., 2017, 53(3):89-96 | DOI: 10.17221/192/2016-CJGPB 
In the past decade, next-generation sequencing (NGS) platforms have changed the impact of sequencing on our knowledge of crop genomes and gene regulation. These techniques are today acquiring a great potential in metagenomic and agrigenomic research while showing prospects for their utilization in plant breeding. We can now obtain new and beneficial information about gene regulation on the cellular as well as whole-plant level through RNA-sequencing and subsequent expression analyses of genes participating in plant defence reactions to pathogens and in abiotic stress tolerance. NGS has facilitated the development of methods to genotype very large numbers...
Comparative transcriptomic analysis reveals a series of single nucleotide polymorphism between red- and white-fleshed loquats (Eriobotrya japonica)Original Paper
Shuxia SUN, Jing LI, Dong CHEN, Hongjiang XIE, Meiyan TU, Liu JIA, Guoliang JIANG, 2*
Czech J. Genet. Plant Breed., 2017, 53(3):97-106 | DOI: 10.17221/43/2016-CJGPB
Loquat (Eriobotrya japonica) is an economically important crop and red-fleshed cultivars have a much higher carotenoid content than white-fleshed cultivars. We used Illumina RNA-seq technology to gain a global overview of the loquat transcriptome from a mixture of fruit samples at different developmental stages for both red-fleshed and white-fleshed loquat. A total of 94.98 million paired-end short reads were obtained and 61 586 unigenes were generated from de novo assembly with an average length of 817 bp. Among these unigenes, 44 710 unigenes were annotated by blast against Nr, Swissprot, GO, COG and KEGG databases. For these annotated...
Changes in stem and spike related traits resulting from breeding in Iranian wheat cultivars: associations with grain yieldOriginal Paper
Mehdi JOUDI, Ali AHMADI, Valiollah MOHAMMADI
Czech J. Genet. Plant Breed., 2017, 53(3):107-113 | DOI: 10.17221/178/2016-CJGPB
This study investigated changes in stem and spike characteristics resulting from breeding in Iranian wheat cultivars, and their relationship with grain yield. Eighty-one wheat cultivars released between 1930 and 2006 were examined under well-watered (WW) and terminal drought stress (DS) conditions in Karaj during 2007-2008 and 2008-2009 and under WW condition at Parsabad in Moghan region during 2010-2011. A genetic improvement over time in stem specific weight (SSW) along with significant positive correlations between this trait and grain yield were found at Karaj under DS conditions and at Parsabad, suggesting that SSW could be used as an indirect...
Germplasm evaluation and molecular selection of potato (Solanum tuberosum L.) cultivars with disease resistance in ChinaOriginal Paper
Wan Lin LI, Wen GUO, Ji Ping XIAO, Lei BAI, Hua Chun GUO
Czech J. Genet. Plant Breed., 2017, 53(3):114-121 | DOI: 10.17221/101/2016-CJGPB
Foreground and background selections are two important aspects that need to be carefully considered by plant breeders during field selection. In this article, we used 7 disease resistance markers, including four late blight and three potato virus disease resistance gene markers, and 12 microsatellite markers to evaluate the disease resistance and genetic diversity of 76 potato cultivars in total which were collected from 15 provinces of China. The foreground selection results showed that a number of materials, clustered separately, contained more than two late blight resistance markers or pyramided late blight and virus disease resistance gene markers...
Pathotypes of wheat leaf rust (Puccinia triticina Eriks.) and resistance of registered cultivars in the Czech Republic in 2012-2015Short Communication
Alena HANZALOVÁ, Pavel BARTO©, Ta»ána SUMÍKOVÁ
Czech J. Genet. Plant Breed., 2017, 53(3):122-126 | DOI: 10.17221/121/2016-CJGPB
In 2012-2015 the virulence of the wheat leaf rust (Puccinia triticina Eriks.) population was studied on Thatcher near-isogenic lines with Lr1, Lr2a, Lr2b, Lr2c, Lr3a, Lr9, Lr10, Lr11, Lr13, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28. Samples of leaf rust were obtained from different parts of the Czech Republic. A total of 163 wheat leaf rust isolates were analysed. No virulence for the resistance gene Lr9 was found. Virulence for Lr19 was found only in one isolate...
Evaluation of variations in plastid DNA non-coding regions in selected species of the genus SolanumShort Communication
Vladimíra SEDLÁKOVÁ, Petr SEDLÁK, Dukagjin ZEKA, Jaroslava DOMKÁŘOVÁ, Petr DOLE®AL, Pavel VEJL
Czech J. Genet. Plant Breed., 2017, 53(3):127-131 | DOI: 10.17221/76/2015-CJGPB
The diversity of three non-coding plastid DNA loci (trnL/trnF spacer, trnV/16SrRNA spacer, trnL/trnL intron) was assessed in 16 Solanum L. species (135 individuals). Polymorphisms were detected by denaturing gradient gel electrophoresis (DGGE) and verified by direct sequencing. No intraspecific diversity and only poor interspecific diversity was detected. Unique S. mochiquense Ochoa specific length polymorphism at the trnL/trnL locus represented by duplication of an 18 bp segment was discovered. The detected DGGE interspecific trnL/trnF locus polymorphism...