Czech J. Genet. Plant Breed., 2017, 53(3):127-131 | DOI: 10.17221/76/2015-CJGPB

Evaluation of variations in plastid DNA non-coding regions in selected species of the genus SolanumShort Communication

Vladimíra SEDLÁKOVÁ*,1, Petr SEDLÁK1, Dukagjin ZEKA2, Jaroslava DOMKÁŘOVÁ3, Petr DOLEŽAL4, Pavel VEJL1
1 Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Prague, Czech Republic
2 Department of Crops, University of Prishtina, Prishtinë, Republic of Kosovo
3 Department of Potato Genetic Resources and
4 Department of Potato Protection, Potato Research Institute, Ltd., Havlíčkův Brod, Czech Republic

The diversity of three non-coding plastid DNA loci (trnL/trnF spacer, trnV/16SrRNA spacer, trnL/trnL intron) was assessed in 16 Solanum L. species (135 individuals). Polymorphisms were detected by denaturing gradient gel electrophoresis (DGGE) and verified by direct sequencing. No intraspecific diversity and only poor interspecific diversity was detected. Unique S. mochiquense Ochoa specific length polymorphism at the trnL/trnL locus represented by duplication of an 18 bp segment was discovered. The detected DGGE interspecific trnL/trnF locus polymorphism did not specifically associate with single point mutations in the sequence confirmed by sequencing. The DGGE method was found to be a simple and cheap pre-exploring tool for mutation detection in compared DNA regions. Some identified polymorphisms can be used in the management of genetic resources.

Keywords: DGGE; DNA sequencing; genetic diversity; point mutations; potato genetic resources

Published: September 30, 2017  Show citation

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SEDLÁKOVÁ V, SEDLÁK P, ZEKA D, DOMKÁŘOVÁ J, DOLEŽAL P, VEJL P. Evaluation of variations in plastid DNA non-coding regions in selected species of the genus Solanum. Czech J. Genet. Plant Breed. 2017;53(3):127-131. doi: 10.17221/76/2015-CJGPB.
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