Czech J. Genet. Plant Breed., 2017, 53(3):114-121 | DOI: 10.17221/101/2016-CJGPB

Germplasm evaluation and molecular selection of potato (Solanum tuberosum L.) cultivars with disease resistance in ChinaOriginal Paper

Wan Lin LI, Wen GUO, Ji Ping XIAO, Lei BAI, Hua Chun GUO*
Tuber and Crop Research Institute, Yunnan Agricultural University, Kunming, Yunnan, P.R. China

Foreground and background selections are two important aspects that need to be carefully considered by plant breeders during field selection. In this article, we used 7 disease resistance markers, including four late blight and three potato virus disease resistance gene markers, and 12 microsatellite markers to evaluate the disease resistance and genetic diversity of 76 potato cultivars in total which were collected from 15 provinces of China. The foreground selection results showed that a number of materials, clustered separately, contained more than two late blight resistance markers or pyramided late blight and virus disease resistance gene markers together. A lot of them were collected from the southwest of China. Additionally, the genetic backgrounds of all cultivars were relatively narrow and a limited number of cultivars (15.8%) contained both potato late blight and potato virus Y resistance markers. Also, only two accessions (Yunshu 103 and Lishu 7) contained both late blight and potato virus X resistance markers. In conclusion, this comprehensive evaluation of genetic resources will shed the light on potato disease resistance breeding in the future.

Keywords: genetic diversity; potato late blight resistance; potato virus X resistance, potato virus Y resistance; SSR markers

Published: September 30, 2017  Show citation

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LI WL, GUO W, XIAO JP, BAI L, GUO HC. Germplasm evaluation and molecular selection of potato (Solanum tuberosum L.) cultivars with disease resistance in China. Czech J. Genet. Plant Breed. 2017;53(3):114-121. doi: 10.17221/101/2016-CJGPB.
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    References

    1. Babu R., Nair S.K., Prasanna B.M., Gupta H.S. (2004): Integrating marker-assisted selection in crop breeding - prospects and challenges. Current Science, 87: 607-619.
    2. Ballvora A., Ercolano M.R., Weiß J., Meksem K., Bormann C.A., Oberhagemann P., Salamini F., Gebhardt C. (2002): The R1 gene for potato resistance to late blight (Phytophthora infestans) belongs to the leucine zipper/NBS/ LRR class of plant resistance genes. The Plant Journal, 30: 361-371. Go to original source... Go to PubMed...
    3. Barone A. (2004): Molecular marker-assisted selection for potato breeding. American Journal of Potato Research, 81: 111-117. Go to original source...
    4. Benbouza H., Jacquemin J.M., Baudoin J.P., Mergeai G. (2006): Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels. Biotechnologie, Agronomie, Société et Environnement, 10: 77-81.
    5. Duan Y.F., Liu J., Bian C.S., Duan S.G., Xu J.F., Jin L.P. (2009): Construction of fingerprinting and analysis of genetic diversity with SSR markers for eighty-eight approved potato cultivars (Solanum tuberosum L.) in China. Acta Agronomica Sinica, 35: 1451-1457. Go to original source...
    6. Feingold S., Lloyd J., Norero N., Bonierbale M., Lorenzen J. (2005): Mapping and characterization of new EST-derived microsatellites for potato (Solanum tuberosum L.). Theoretical and Applied Genetics, 111: 456-466. Go to original source... Go to PubMed...
    7. Gebhardt C., Valkonen J.P.T. (2001): Organization of genes controlling disease resistance in the potato genome. Annual Review of Phytopathology, 39: 79-102. Go to original source... Go to PubMed...
    8. Ghislain M., Spooner D.M., Rodriguez F., Villamón F., Nunez J., Vásquez C., Waugh R., Bonierbale M. (2004): Selection of highly informative and user-friendly microsatellites (SSRs) for genotyping of cultivated potato. Theoretical and Applied Genetics, 108: 881-890. Go to original source... Go to PubMed...
    9. Hooks C.R., Fereres A. (2006): Protecting crops from nonpersistently aphid-transmitted viruses: a review on the use of barrier plants as a management tool. Virus Research, 120: 1-16. Go to original source... Go to PubMed...
    10. Huang S., Van Der Vossen E.A., Kuang H., Vleeshouwers V.G., Zhang N., Borm T.J., Van Eck H.J., Baker B., Jacobsen E., Visser R.G. (2005): Comparative genomics enabled the isolation of the R3a late blight resistance gene in potato. The Plant Journal, 42: 251-261. Go to original source... Go to PubMed...
    11. Iqbal N., Tabasum A., Sayed H., Hameed A. (2009): Evaluation of genetic diversity among bread wheat varieties and landraces of Pakistan by SSR markers. Cereal Research Communications, 37: 489-498. Go to original source...
    12. Jansky S.H., Jin L.P., Xie K.Y., Xie C.H., Spooner D.M. (2009): Potato production and breeding in China. Potato Research, 52: 57-65. Go to original source...
    13. Kamoun S. (2001): Nonhost resistance to Phytophthora: novel prospects for a classical problem. Current Opinion in Plant Biology, 4: 295-300. Go to original source... Go to PubMed...
    14. Kasai K., Morikawa Y., Sorri V.A., Valkonen J.P.T., Gebhardt C., Watanabe K.N. (2000): Development of SCAR markers to the PVY resistance gene Ryadg based on a common feature of plant disease resistance genes. Genome, 43: 1-8. Go to original source...
    15. Lawson C., Kaniewski W., Haley L., Rozman R., Newell C., Sanders P., Tumer N.E. (1990): Engineering resistance to mixed virus infection in a commercial potato cultivar: resistance to potato virus X and potato virus Y in transgenic Russet Burbank. Nature Biotechnology, 8: 127-134. Go to original source... Go to PubMed...
    16. Li G., Huang S., Guo X., Li Y., Yang Y., Guo Z., Kuang H., Rietman H., Bergervoet M., Vleeshouwers V.G., Van der Vossen E.A., Qu D., Visser R.G., Jacobsen E., Vossen J.H. (2011): Cloning and characterization of R3b; members of the R3 superfamily of late blight resistance genes show sequence and functional divergence. Molecular PlantMicrobe Interactions, 24: 1132-1142. Go to original source... Go to PubMed...
    17. Liao H., Guo H. (2014): Using SSR to evaluate the genetic diversity of potato cultivars from Yunnan Province (SW China). Acta Biologica Cracoviensia Series Botanica, 56: 1-12. Go to original source...
    18. Min X. (2008): Genetic relationship and diversity of approved potato cultivars (Solanum tuberosum L.) in China. [Master Thesis.] Beijing, School of Chinese Academy of Agricultural Sciences.
    19. Moisan-Thiery M., Marhadour S., Kerlan M.C., Dessenne N., Perramant M., Gokelaere T., Le Hingrat Y. (2005): Potato cultivar identification using simple sequence repeats markers (SSR). Potato Research, 48: 191-200. Go to original source...
    20. Mori K., Sakamoto Y., Mukojima N., Tamiya S., Nakao T., Ishii T., Hosaka K. (2011): Development of a multiplex PCR method for simultaneous detection of diagnostic DNA markers of five disease and pest resistance genes in potato. Euphytica, 180: 347-355. Go to original source...
    21. Mori K., Mukojima N., Nakao T., Tamiya S., Sakamoto Y., Sohbaru N., Hayashi K., Watanuki H., Nara K., Yamazaki K., Ishii T., Hosaka K. (2012): Germplasm release: Saikai 35, a male and female fertile breeding line carrying Solanum phureja-derived cytoplasm and potato cyst nematode resistance (H1) and Potato virus Y resistance (Rychc) genes. American Journal of Potato Research, 89: 63-72. Go to original source...
    22. Ojiambo P.S., Nyanapah J.O., Lung'Aho C., Karinga J.K., Kidanemariam H.M. (2000): Comparing different epidemiological models in field evaluations of selected genotypes from Solanum tuberosum CIP population A for resistance to Phytophthora infestans (Mont.) De Bary in Kenya. Euphytica, 111: 211-219. Go to original source...
    23. Ortega F., Lopez-Vizcon C. (2012): Application of molecular marker-assisted selection (MAS) for disease resistance in a practical potato breeding programme. Potato Research, 55: 1-13. Go to original source...
    24. Ottoman R.J., Hane D.C., Brown C.R., Yilma S., James S.R., Mosley A.R., Crosslin J.M., Vales M.I. (2009): Validation and implementation of marker-assisted selection (MAS) for PVY resistance (Ry adg gene) in a tetraploid potato breeding program. American Journal of Potato Research, 86: 304-314. Go to original source...
    25. Reid A., Kerr E.M. (2007): A rapid simple sequence repeat (SSR)-based identification method for potato cultivars. Plant Genetic Resources, 5: 7. Go to original source...
    26. Solomon-Blackburn R.M., Barker H. (2001): Breeding virus resistant potatoes (Solanum tuberosum): a review of traditional and molecular approaches. Heredity, 86: 17-35. Go to original source... Go to PubMed...
    27. Tai G.C., Young D.A. (1984): Early generation selection for important agronomic characteristics in a potato breeding population. American Journal of Potato Research, 61: 419-434. Go to original source...
    28. White S., Shaw D. (2010): Breeding for host resistance: the key to sustainable potato production. PPO-Special Report, 14: 125-130.
    29. Xiong G., Wang S., Shang K., Zhuang S., Zhang Z. (2012): Climatic characteristics of summer precipitation in southwest China in the past 50 years. Journal of Lanzhou University, 48: 45-52. (in Chinese)

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