Czech Journal of Genetics and Plant Breeding, 2020 (vol. 56), issue 1

A breeding concept to improve the performance of locally cultivated bread wheat (Triticum aestivum L.) cultivarsOriginal Paper

Vasileios Greveniotis, Stylianos Zotis, Evangelia Sioki, Constantinos Ipsilandis

Czech J. Genet. Plant Breed., 2020, 56(1):1-8 | DOI: 10.17221/47/2019-CJGPB  

The objective of this study was to explore the internal variability in six established F7 commercial wheat cultivars for breeding purposes. They are sown traditionally in the region of Western Macedonia, Greece. Spikes of the six cultivars were sown in 2008 in separate rows. A head to row selection scheme was applied for two growing periods in order to select lines within the cultivars, based on various traits such as: the total spike number, the spike weight per row, the 1000-kernel weight and the specific weight . The final selection was based on the specific weight and the four best rows from each cultivar were selected. All selected lines were...

Reaction of winter wheat cultivars to eyespot assessed visually and by real-time PCROriginal Paper

Jana Palicová, Pavel Matušinsky, Alena Hanzalová, Ivana Svačinová, Veronika Dumalasová, Jana Chrpová

Czech J. Genet. Plant Breed., 2020, 56(1):9-14 | DOI: 10.17221/52/2019-CJGPB  

The reaction of twelve winter wheat cultivars frequently grown in the Czech Republic and twenty-five new breeding lines to inoculation with Oculimacula yallundae and Oculimacula acuformis was evaluated in small plot trials from 2017-2018. The assessment was carried out visually by symptoms and by a quantitative real-time polymerase chain reaction (qPCR). The aims of the study were to compare the results of both methods, to evaluate the effect of the resistance gene Pch1 to eyespot, and to select new breeding lines resistant to eyespot. The relationship between the eyespot symptoms and the pathogen DNA content in plant tissues...

Comprehensive genomic analysis and expression profiling of the BTB and TAZ (BT) genes in cucumber (Cucumis sativus L.)Original Paper

Yong Zhou, Guanghua Li, Lin Zhang, Jie Xu, Lifang Hu, Lunwei Jiang, Shiqiang Liu

Czech J. Genet. Plant Breed., 2020, 56(1):15-23 | DOI: 10.17221/34/2019-CJGPB  

BTB-TAZ (BT) proteins are plant-specific transcription factors containing a BTB domain and a TAZ domain. They play vital roles in various biological processes and stress responses. In this study, a total of three BT genes (CsBT1-3) were identified from cucumber genome, and they were unevenly distributed in two of the seven chromosomes. Phylogenetic analysis of the BT proteins from cucumber, Arabidopsis, apple, tomato, and rice revealed that these proteins could be distinctly divided into two groups in accordance with their motif distributions. We also determined the structures of BT genes from cucumber,...

Comparative metabolomics and transcriptomics of pistils, stamens and pistilloid stamens widen key knowledge of pistil and stamen development in wheat.Original Paper

Yan Yu, Zheng Song Peng, Ji Peng Qu, Zhen Yong Chen, Shu Hong Wei, Ming Li Liao, Li Zhang, Zai Jun Yang

Czech J. Genet. Plant Breed., 2020, 56(1):24-33 | DOI: 10.17221/70/2019-CJGPB  

To examine the role of metabolites in wheat stamen and pistil development, metabolomic analyses of pistilloid stamens (PS), pistils (P), and stamens (S) from a novel wheat mutant homologous transformation sterility-1 (HTS-1) and controls from their sib-line CSTP were conducted using base gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). Then, the metabolomic data were integrated with previously published transcriptomic data and analysed. In total, 141 annotated metabolites were determined from P, PS and S tissues by comparison with reference standards. A total of 90, 93 and 18 different metabolites were...

The application of an Agrobacterium-mediated in planta transformation system in a Catharanthus roseus medicinal plantOriginal Paper

Zahra Bahari, Sima Sazegari, Ali Niazi, Alireza Afsharifar

Czech J. Genet. Plant Breed., 2020, 56(1):34-41 | DOI: 10.17221/153/2018-CJGPB  

The lack of an efficient protocol for the transformation of Catharanthus roseus, as an important medicinal plant, causes obstacles in the genetic engineering programmes of this plant. Therefore, the present study was conducted to examine a convenient and reliable system for generating stable transgenic lines based on the in planta method. To do so, two different in planta Agrobacterium infection systems were investigated. The transgenic lines were produced through ovary injection and shoot apical meristem (SAM) immersing methods. The presence and expression of the GFP transgene in the T0 and T1...