Czech J. Genet. Plant Breed., 2020, 56(1):9-14 | DOI: 10.17221/52/2019-CJGPB

Reaction of winter wheat cultivars to eyespot assessed visually and by real-time PCROriginal Paper

Jana Palicová*,1, Pavel Matušinsky2,3, Alena Hanzalová1, Ivana Svačinová2, Veronika Dumalasová1, Jana Chrpová1
1 Crop Research Institute, Prague, Czech Republic
2 Agrotest Fyto, Ltd., Kroměříž, Czech Republic
3 Department of Botany, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic

The reaction of twelve winter wheat cultivars frequently grown in the Czech Republic and twenty-five new breeding lines to inoculation with Oculimacula yallundae and Oculimacula acuformis was evaluated in small plot trials from 2017-2018. The assessment was carried out visually by symptoms and by a quantitative real-time polymerase chain reaction (qPCR). The aims of the study were to compare the results of both methods, to evaluate the effect of the resistance gene Pch1 to eyespot, and to select new breeding lines resistant to eyespot. The relationship between the eyespot symptoms and the pathogen DNA content in plant tissues followed a moderate linear regression. Low levels of eyespot were observed in the cultivars/lines possessing the resistance gene Pch1 (Annie, Hermann, Rebell, SG-S1215-14, SG-S1825-14, SG-S791-13) and also in the line SG-SU630-15. The qPCR method was able to detect low levels of the pathogens in the plant tissue and to distinguish two eyespot pathogens. O. acuformis was detected in very low concentrations in the inoculated plants compared with O. yallundae. The eyespot infection rate was significantly higher in 2017 than in the next agricultural season due to extremely dry and warm spring weather in 2018.

Keywords: molecular markers; Oculimacula acuformis; Oculimacula yallundae; Pch1 gene

Published: March 31, 2020  Show citation

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Palicová J, Matušinsky P, Hanzalová A, Svačinová I, Dumalasová V, Chrpová J. Reaction of winter wheat cultivars to eyespot assessed visually and by real-time PCR. Czech J. Genet. Plant Breed. 2020;56(1):9-14. doi: 10.17221/52/2019-CJGPB.
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    References

    1. Burt C., Hollins T.W., Nicholson P. (2011): Identification of a QTL conferring seedling and adult plant resistance to eyespot on chromosome 5A of Cappelle Desprez. Theoretical and Applied Genetics, 122: 119-128. Go to original source... Go to PubMed...
    2. Cadle M.M. (1997): Identification and genetic characterization of resistance to Pseudeocercosporella herpotrichoides in diploid wheat relatives Triticum monococcum and T. tauschii. [MSc Thesis] Washington State University. Go to original source...
    3. de la Peña R.C., Murray T.D., Jones S.S. (1996): Linkage relations among eyespot resistance gene Pch2, endopeptidase Ep-A1b, and RFLP marker Xpsr121 on chromosome 7A o wheat. Plant Breeding, 115: 273-275. Go to original source...
    4. Dumalasová V., Palicová J., Hanzalová A., Bížová I., LeišováSvobodová L. (2015): Eyespot resistance gene Pch1 and methods of study of its effectiveness in wheat cultivars. Czech Journal of Genetics and Plant Breeding, 51: 166-173. Go to original source...
    5. Figliuolo G., Jones S.S., Murray T.D., Spagnoletti Zeuli P.L. (1998): Characterization of tetraploid wheat germplasm for resistance to Pseudocercosporella herpotrichoides, cause of eyespot disease. Journal of Genetic Resources and Crop Evolution, 44: 47-56. Go to original source...
    6. Law C.N., Scott P.R., Worland A.J., Hollins T.W. (1975): The inheritance of resistance to eyespot (Cercosporella herpotrichoides) in wheat. Genetics Research, 25: 73-79. Go to original source...
    7. Leroux P., Gredt M., Remuson F., Micoud A., Walker A.-S. (2012): Fungicide resistance status in French populations of the wheat eyespot fungi Oculimacula acuformis and Oculimacula yallundae. Pest Management Science, 69: 15-26. Go to original source... Go to PubMed...
    8. Lucas J.A., Dyer P.S., Muray T.D. (2000): Pathogenicity, host specificity, and population biology of Tapesia spp., causal agents of eyespot disease in cereals. Advances in Botanical Research, 33: 226-258. Go to original source...
    9. Maia N. (1967): Obtention de blés tendres résistants au piétin-verse (Cercosporella herpotrichoides) par croisements interspécifiques blés × Aegilops. Comptes Rendus de l'Academie d'Agriculture de France, 53: 149-154.
    10. Matusinsky P., Mikolasova R., Spitzer T., Klem K. (2008): Colonization of winter wheat stem bases by communities of pathogenic fungi. Cereal Research Communications, 36: 77-88. Go to original source...
    11. Matusinsky P., Mikolasova R., Klem K., Spitzer T. (2009): Eyespot infection risks on wheat with respect to climatic conditions and soil management. Journal of Plant Pathology, 91: 93-101.
    12. Matusinsky P., Svacinova I., Jonaviciene A., Tvarůžek L. (2017): Long-term dynamics of causative agents of stem base diseases in winter wheat and reaction of Czech Oculimacula spp. and Microdochium spp. populations to prochloraz. European Journal of Plant Pathology, 148: 199-206. Go to original source...
    13. Meyer N., Lind V., Heindorf M., Korzun V., Triedr W., Ordon F. (2011): Diagnostic value of molecular markers linked to the eyespot resistance gene Pch1 in wheat. Euphytica, 177: 267-275. Go to original source...
    14. Murray T.D., de la Peña R.C., Yildirim A., Jones S.S. (1994): A new source of resistance to Pseudocercosporella herpotrichoides, cause of eyespot disease of wheat, located on chromosome 4V of Dasypyrum villosum. Plant Breeding, 113: 281-286. Go to original source...
    15. Palicová J., Matušinsky P. (2019): Reaction of eyespot causal agents to some active ingredients of fungicides in vitro. Cereal Research Communications, 47: 88-97. Go to original source...
    16. Ramanauskienė J., Gaurilčikienė I., Supronienė S., Ronis A., Česnulevičienė R. (2014): Evaluation of eyespot incidence and structure of Oculimacula spp. population in winter rye in Lithuania. Zemdirbyste-Agriculture, 101: 425-430. Go to original source...
    17. Saur L., Cavelier N. (1995): Caractéristiques d´isolats de Pseudocercosporella herpotrichoides provenant de variétés de blé posédant le gene de résistance Pch1. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz, 102: 472-477.
    18. Turner A.S., O´Hara R.B., Rezanoor H.N., Nutall M., Smith J.N., Nicholson P. (1999): Visual disease and PCR assessment of stem base diseases in winter wheat. Plant Pathology, 48: 742-748. Go to original source...
    19. Turner A.S., Nicholson P., Edwards S.G. et al. (2001): Evaluation of diagnostic and quantitative PCR for the identification and severity assessment of eyespot and sharp eyespot in winter wheat. Plant Pathology, 50: 463-469. Go to original source...
    20. Walsh K., Korimbocus J., Boonham N., Jennings P., Hims M. (2005): Using real-time PCR to discriminate and quantify the closely related wheat pathogens Oculimacula yallundae and Oculimacula acuformis. Journal of Phytopathology, 153: 715-721. Go to original source...
    21. Wei L., Muranty H., Zhang H. (2011): Advances and prospects in wheat eyespot research: contributions from genetics and molecular tools. Journal of Phytopathology, 159: 457-470. Go to original source...
    22. Worland A.J., Law C.N., Hollins T.W., Kochner R.M.D., Guira A. (1988): Location of a gene for resistance to eyespot (Pseudocercosparella herpotrichoides) on chromosome 7D of bread wheat. Plant Breeding, 101: 43-51. Go to original source...

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