Czech J. Genet. Plant Breed., 2020, 56(1):15-23 | DOI: 10.17221/34/2019-CJGPB

Comprehensive genomic analysis and expression profiling of the BTB and TAZ (BT) genes in cucumber (Cucumis sativus L.)Original Paper

Yong Zhou#,1,2, Guanghua Li#,2, Lin Zhang2, Jie Xu1,3, Lifang Hu1,3, Lunwei Jiang2, Shiqiang Liu*,2
1 Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, P.R. China
2 College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, P.R. China
3 College of Agronomy, Jiangxi Agricultural University, Nanchang, P.R. China

BTB-TAZ (BT) proteins are plant-specific transcription factors containing a BTB domain and a TAZ domain. They play vital roles in various biological processes and stress responses. In this study, a total of three BT genes (CsBT1-3) were identified from cucumber genome, and they were unevenly distributed in two of the seven chromosomes. Phylogenetic analysis of the BT proteins from cucumber, Arabidopsis, apple, tomato, and rice revealed that these proteins could be distinctly divided into two groups in accordance with their motif distributions. We also determined the structures of BT genes from cucumber, Arabidopsis, and rice to demonstrate their differences. The quantitative real-time PCR (qRT-PCR) results showed that the CsBT genes displayed differential expression patterns in cucumber tissues, and their expression was regulated by cold, salt, and drought stresses. These findings suggest that CsBT genes may participate in cucumber development and responses to various abiotic stresses.

Keywords: abiotic stress; BTB domain; CaMBD domain; gene expression; TAZ domain

Published: March 31, 2020  Show citation

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Zhou Y, Li G, Zhang L, Xu J, Hu L, Jiang L, Liu S. Comprehensive genomic analysis and expression profiling of the BTB and TAZ (BT) genes in cucumber (Cucumis sativus L.). Czech J. Genet. Plant Breed. 2020;56(1):15-23. doi: 10.17221/34/2019-CJGPB.
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