Czech Journal of Genetics and Plant Breeding, 2018 (vol. 54), issue 4
Index of Volume 54Index
editors
Czech J. Genet. Plant Breed., 2018, 54(4):I-IV | DOI: 10.17221/11393-CJGPB 
Structural and functional apple genomics and its application in breedingReview
Sylwia Elzbieta KELLER-PRZYBYŁKOWICZ
Czech J. Genet. Plant Breed., 2018, 54(4):147-153 | DOI: 10.17221/59/2017-CJGPB
The present paper summarized some knowledge of modern technologies, applied in apple (Malus domestica) genome studies. New generation sequencing allowed single nucleotide polymorphism (SNP) chip technologies for genotyping, description of functional apple genes, characterization of the evolutionary results in apple genome fragment transition, as well as phylogenetic reconstruction of the genus Malus, being the confirmed progeny of M. sieviersii. Based on these technologies, newly developed putative markers may give the most important biological data such as age, geographical origins, tissue type determination, and external...
Identification of molecular markers associated with genic male sterility in tetraploid cotton (Gossypium hirsutum L.) through bulk segregant analysis using a cotton SNP 63K arrayOriginal Paper
Dharmalingam RAJA, Marappan SARVANA KUMAR, Ponnuswamy RENUKA DEVI, Sankaran LOGANATHAN, Kamalasekharan RAMYA, Nallathambi KANNAN, Vaidyanathan SUBRAMANIAN
Czech J. Genet. Plant Breed., 2018, 54(4):154-160 | DOI: 10.17221/25/2017-CJGPB
Genic male sterility (GMS) is one of the most important economic traits for cotton (Gossypium hirsutum L.) hybrid seed production. The GMS trait conferred by two recessive alleles ms5 and ms6 in homozygous constitution is widely used for cotton hybrid seed production in India. Identification of molecular markers closely linked to the ms5 and ms6 alleles would be useful in effective transferring in a short time male-sterility genes into cultivars or elite lines using marker-assisted backcrossing. Here, we describe a quick method to identify markers for GMS genes using bulk segregant analysis (BSA) in the...
Regeneration and Agrobacterium-mediated transformation of japonica rice varieties developed for a cold regionOriginal Paper
Mingfang FENG, Jing CANG, Junhong WANG, Jian SUN, Jing YU, Qinghua XU, Da ZHANG, Ning YANG, Qiuwei LU, Yan LV
Czech J. Genet. Plant Breed., 2018, 54(4):161-167 | DOI: 10.17221/86/2017-CJGPB
So far, a large number of transformation systems have been established for japonica rice, but only a few have been reported for cold-region varieties. In our study, we established highly efficient tissue culture systems for two cold-region rice cultivars, Dongnong 427 and Longdao 14. Plant growth regulator (PGR) levels were optimized by an orthogonal experimental design. The culture ability, constituted by induction and differentiation rate, served as the detection index of orthogonal experiments. The optimal combinations of PGRs for callus induction and regeneration of Dongnong 427 and Longdao 14 were 1 mg/l 2,4-dichlorophenoxyacetic acid...
Genome-wide analysis of the WRKY family genes and their responses to cold stress in watermelonOriginal Paper
Yan-Man LI, Lei ZHU, Hua-Yu ZHU, Peng-Yao SONG, Lu-Qin GUO, Lu-Ming YANG
Czech J. Genet. Plant Breed., 2018, 54(4):168-176 | DOI: 10.17221/72/2017-CJGPB
The WRKY transcription factors play important roles in various physiological processes, especially in regulating plant resistance to environmental stresses. Watermelon (Citrullus lanatus) plants suffer from various stressful climate conditions during their growth, especially cold stress. However, little information about the exact role of WRKYs in watermelon responses to cold was available. In this study, a total of 57 candidate ClWRKY genes from watermelon genome were identified and they were distributed unevenly on 11 chromosomes. After excluding five ClWRKY genes with incomplete WRKY domains, phylogenetic analysis showed...
Correlations between SmCPS1 promoter polymorphism and tanshinone contents in Salvia miltiorrhizaOriginal Paper
Ying SUN, Xin CHEN, Xiao-Yan GAN, Zhu-Yun YAN, Di-Xiu MU, Qing-Rong WANG
Czech J. Genet. Plant Breed., 2018, 54(4):177-182 | DOI: 10.17221/97/2017-CJGPB
Copalyl diphosphate synthase 1 (SmCPS1) is the first committed enzyme in tanshinone biosynthesis. The promoter region plays an important role in the transcriptional regulation of genes. Mutations in the promoter region may affect gene expression, resulting in changes in the amount of metabolites. In this study, we investigated the SmCPS1 gene promoter region together with the 388 bp downstream from the translation start site and the content of tanshinones of 12 different genotypes of Salvia miltiorrhiza. The cis-elements of SmCPS1 promoter were predicted and analysed by the Plant Transcriptional Regulatory Map database....
In vitro micropropagation in Boehmeria nivea to generate safe planting materials for large-scale cultivationOriginal Paper
Pranit Kumar MUKHERJEE, Raju MONDAL, Sourav DUTTA, Kanti MEENA, Madhumita ROY, Asit Baran MANDAL
Czech J. Genet. Plant Breed., 2018, 54(4):183-189 | DOI: 10.17221/79/2017-CJGPB
An efficient in vitro micropropagation protocol has been developed using nodal explants of ramie (Boehmeria nivea), with maximum shoots (42) per explant in 5 passages (passage duration: 21 days) on Murashige and Skoog medium supplemented with 2.0 mg/l 6-benzyladenine and 2.0 mg/l AgNO3. 1/2 Murashige and Skoog medium containing 40% sucrose was found to be most effective for the rooting of in vitro developed shoots. Those plantlets were acclimatized and transferred to pots for hardening under glasshouse conditions. About 91% of mericlones survived and showed no ectopic expression in respect of any morphological...
Estimations of fibre trait stability and type of inheritance in cottonShort Communication
Vasileios GREVENIOTIS, Evangelia SIOKI, Constantinos G. IPSILANDIS
Czech J. Genet. Plant Breed., 2018, 54(4):190-192 | DOI: 10.17221/12/2017-CJGPB
Traits affecting fibre quality were evaluated in a multi-location environmental experiment. Four main cotton regions in Greece were selected as different environments. Five commercial cotton cultivars were used for evaluation of 10 fibre quality traits. Each cultivar was sown in 10 different fields in each region. Environmental fluctuations within regions affected each quality trait differently showing a different degree of inheritance. Four traits showed the lowest stability index values indicating quantitative inheritance, further four traits with intermediate values indicated determination by a few genes, while the more stable and thus with qualitative...