Czech J. Genet. Plant Breed., 2018, 54(4):147-153 | DOI: 10.17221/59/2017-CJGPB
Structural and functional apple genomics and its application in breedingReview
- Research Institute of Horticulture, Skierniewice, Poland
The present paper summarized some knowledge of modern technologies, applied in apple (Malus domestica) genome studies. New generation sequencing allowed single nucleotide polymorphism (SNP) chip technologies for genotyping, description of functional apple genes, characterization of the evolutionary results in apple genome fragment transition, as well as phylogenetic reconstruction of the genus Malus, being the confirmed progeny of M. sieviersii. Based on these technologies, newly developed putative markers may give the most important biological data such as age, geographical origins, tissue type determination, and external visible characters. The new generation genotyping platforms, representing very high efficiency, are now successfully applied for random apple genome-wide association (GWA) studies as well pedigree-based analysis and marker-assisted selection (MAS).
Keywords: genotyping; GWA; high-throughput sequencing; fine mapping; MAS
Published: December 31, 2018 Show citation
References
- Bai Y., Dougherty L., Xu K. (2014): Towards an improved apple reference transcriptome using RNA-seq. Molecular Genetics and Genomics, 289: 472-438.
Go to original source...
Go to PubMed...
- Baldo A., Norelli J.L., Farrell Jr. R.E., Basset C.L., Aldwinckle H.S., Malnoy M. (2010): Identification of genes differentially expressed during interaction of resistance and susceptible apple cultivars (Malus × domestica) with Erwinia amylovora. BMC Plant Biology, 10: 1-10.
Go to original source...
Go to PubMed...
- Bianco L., Cerstaro A., sergeant D.J., Banchi E., Derdak S., Di Guardi M., Salvi S., Jansen J., Vila R., Gut I., Laurence F., Chagne D., Velasco R., Van de Weg E., Troggio M. (2014): Development and validation of a 20K single nucleotide polymorphism (SNP) whole genome genotyping array for apple (Malus × domestica Borkh.). PLoS ONE, 9: e110377.
Go to original source...
Go to PubMed...
- Bianco L., Cestaro A., Linsmith G., Muranty H., Denace C., Theron A., Poncet C., Micheletti D., Kerschbamer E., Di Piero E.A., Langer S., Pindo M., Van de Weg E., Davassi A., Laurens F., Velasco R., Durel C-E., Troggio M. (2016): Development and validation of Axiom Apple 480K SNP genotyping array. The Plant Journal, 86: 62-74.
Go to original source...
Go to PubMed...
- Boocock J., Chagne D., Marriman T.R., Balck M.A. (2015): The distribution and impact of common copy-number variation in the genome of the domesticated apple, Malus × domestica Borkh. BMC Genomics, 16: 848.
Go to original source...
Go to PubMed...
- Chagne D., Carlisle C.M., Blond C., Volz R.K., Withworth C.J., Oraguzie C.N., Crowhurst R.N., Allan A.C., Espley R.V., Hellens R.P., Gardiner S.E. (2007): Mapping a candidate gen (MdMYB10) for flesh and foliage colour in apple. BMC Genomics, 8: 212.
Go to original source...
Go to PubMed...
- Chagne D., Crowhurst R.N., Troggio M., Davey M.W., Gilmore B., Lawley C., Vanderzande S., Hellens R.P., Kumar S., Cestaro A., Velasco R., Main D., Rees J.D., Iezzoni A., Mockler T., Wilhelm L., Van de Weg E., Gardiner S.E., Bassil N., Peace C. (2012): Genome - wide SNP detection, validation, and development of an 8K SNP array for apple. PLoS ONE, 7: e31745.
Go to original source...
Go to PubMed...
- Chagne D., Lin Wang K., Espley R.V., Volz R.K., How N.M., Rouse S., Brendolise C., Carlisle C.M., Kumar S., De Silva N., Micheletti D., McGhie T., Crowhurst R.N., Storey R.D., Velasco R., Hellens R.P., Gardiner S.E., Allan A.C. (2013): An ancient duplication of apple MYB transcription factors is responsible for novel red fruit flesh phenotypes. Plant Physiology, 161: 225-239.
Go to original source...
Go to PubMed...
- Clarke J.D., Zhu T. (2006): Microarray analysis of the transcriptome as a stepping stone towards understanding biological systems: practical considerations and perspectives. Plant Journal, 45: 630-650.
Go to original source...
Go to PubMed...
- Conrad D.F., Pinto D., Redon R., Feuk L., Gokcumen O., Zang Y., Aerts J., Andrews T.D., Barnes C., Campbell P.,Fitzgerald T., Hu M., Ihm C.H., Kristiansson K., MacArthu D.G., MacDonald J.R., Onyiah I., Pang A.W.C, Robson S., Stirrups K., Valsesia A., Walter K., We J.I., Tyler-Smith C., Carter N.P., Lee C., Scherer S.W., Hurles M.E. (2009): Origin and the functional impact of copy number variation in human genome. Nature, 464: 704-712.
Go to original source...
Go to PubMed...
- Eisen M.B., Brown P.O. (1999): DNA arrays for analysis of gene expression. Methods in Enzymology, 303: 179-205.
Go to original source...
Go to PubMed...
- Ekblom R., Galindo J. (2011): Application of next generation sequencing in molecular ecology of non-model organisms. Heredity, 107: 1-15.
Go to original source...
Go to PubMed...
- Gapper N.E., Giovannoni J.J., Watkins C.B. (2014): Understanding development and ripening of fruit crops in an 'omics' era. Horticulture Research, 1: 14034.
Go to original source...
Go to PubMed...
- Girard C.L., Rombaldi C.V., Dal Cero J., Nobile P.M., Laurens F., Bouzayen M. (2013): Genome-wide analysis of the AP2/ERF superfamily in apple and transcriptional evidence of ERF involvement in scab pathogenesis. Scientia Horticulturae, 151: 112-121.
Go to original source...
- Goulao L.F., Oliveira C.M. (2007): Molecular identification of novel differentially expressed mRNAs up regulated during ripening of apples. Plant Science, 172: 306-318.
Go to original source...
- Han Y., Korban S.S. (2008): An overview of apple genome through BAC end sequence analysis. Plant Molecular Biology, 67: 581-588.
Go to original source...
Go to PubMed...
- Jakovljevic V., Otten P., Berwarth C., Jelkman W. (2017): Analysis of the apple rubbery woody disease by next generation sequencing of total RNA. European Journal of Plant Pathology, 148: 637-646.
Go to original source...
- Janssen J.B., Thodey K., Schaffer R.J., Alba R., Bala-krishnan L., Bishop R., Bowen J.H., Crowhurst R.N., Gleave A.P., Lendger S., McArtney S., Pichler F.B., Snowden K.C., Ward S. (2008): Global gene expression analysis of apple fruit development from the floral bud to ripe fruit. BMC Plant Biology, 8: 16.
Go to original source...
Go to PubMed...
- Kamber T., Buchmann J.P., Porthier J.F., Smits T.H.M., Wicker T., Duffy B. (2016): Fire blight disease reactome: RNA seq transcriptional profile of apple host plant defense response to Erwinia amylovora pathogen infection. Nature: Scientific Reports, 6: 21600.
Go to original source...
Go to PubMed...
- Khan M.A., Han Y., Zhao Y., Korban S.S. (2012): A highthroughput apple SNP genotyping platform using GoldenGate assay. Gene, 494: 196-201.
Go to original source...
Go to PubMed...
- Koboldt D.C., Steinberg K.M., Larson D.E., Wilson R.K., Mardis E.R. (2013): The next-generation sequencing revolution and its impact on genomics. Cell, 155: 27-38.
Go to original source...
Go to PubMed...
- Krost C., Petersen R., Schmidt E.R. (2012): The transcriptomes of columnar type apple trees (Malus domestica) - a comparative study. Gene, 498: 223-230.
Go to original source...
Go to PubMed...
- Krost C., Petersen R., Lokan S., Brauksiepe B., Braun P., Schmidt E.R. (2013): Evaluation of the hormonal state of columnar apple trees (Malus × domestica) base on high throughput gene expression studies. Plant Molecular Biology, 81: 211-220.
Go to original source...
Go to PubMed...
- Kumar S., Chagne D., Bink M.C.A., Volz R.K., Whitwirth C., Carlisle C. (2012a): Genomic selection for fruit quality traits in apple (Malus × domestica Borkh.). PLoS ONE, 7: e36674.
Go to original source...
Go to PubMed...
- Kumar S., Banks T.W., Cloutier S. (2012b): SNP discovery through next-generation sequencing and its application. International Journal of Plant Genomics, 831460: 1-15.
Go to original source...
Go to PubMed...
- Kumar S., Garrick D.J., Bink M., Whitworth C., Chagne D., Volz R.K. (2013): Novel genomic approaches unravel genetic architecture of complex traits in apple. BMC Genomics, 14: 393.
Go to original source...
Go to PubMed...
- Leforestier D., Ravon E., Muranty H., Cornille A., Lemaire C., Giraud T., Durel C.E., Branca A. (2015): Genomic basis of the differences between cider and dessert apple varieties. Evolutionary Applications, 8: 650-661.
Go to original source...
Go to PubMed...
- Lehman R.I. (2008): Historical perspective: Arthur Kornberg, a giant of 20 th century biochemistry. Trends in Biochemical Science, 33: 291-296.
Go to original source...
Go to PubMed...
- Lehman I.R., Bessman M.J., Simms E.S., Kornberg A. (1958): Enzymatic synthesis of deoxyribonucleic acid. (I). Preparation of substrates and partial purification of an enzyme from Escherichia coli. The Journal of Biological Chemistry, 233: 163-170.
Go to original source...
- Linnarsson S. (2010): Recent advances in DNA sequencing methods-general principles of sample preparation. Experimental Cell Research, 316: 1339-1343.
Go to original source...
Go to PubMed...
- Liu Y., Lan J., Li Q., Zhang Y., Wang C., Dai H. (2017): Rapid location of Glomerella leaf spot resistance gene locus in apple by whole genome resequencing. Molecular Breeding, 37: 96.
Go to original source...
- Lopez-Maestre H., Brinza L., Marchet C., Kielbassa J., Bastien S., Boutigny M., Monnin D., Filali A.E, Carareto C.M., Vieira C., Picard F., Kremer N., Vavre F., Sagot M.F, Lacroix V. (2016): SNP calling from RNA-seq data without a reference genome: identification, quantification, differential analysis and impact on the protein sequence. Nucleic Acid Research, 1: 1-13.
Go to original source...
Go to PubMed...
- Maliepaard C., Aston F.H., van Arkel G., Brown L.M., Chevereau E., Dunemann F., Evans K.M., Gardiner S., Guilford P., van Heusden A.W., Janse J., Laurens F., Lynn J.R., Manganaris A.G., den Nijs A.P.M., Periam N., Rikkerink E., Roche P., Ryder C., Sansavini S., Schmidt H., Tartarini S., Verhaegh J.J., Vrielink-van Ginkel M., King G.J. (1998): Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers. Theoretical and Applied Genetics, 97: 60-73.
Go to original source...
- Maxam A.M., Gilbert W. (1977): A new method for sequencing DNA. Proceedings of the National Academy of Science, 74: 560-564.
Go to original source...
Go to PubMed...
- Micheletti D., Troggio M., Zharkikh A., Costa F., Malnoy M., Velasco R., Salvi S. (2011): Genetic divesresity of the genus Malus and implication of linkage mapping with SNPs. Tree Genetics and Genomes, 7: 857-868.
Go to original source...
- Munoz-Amatriain M., Eichten S.R., Wicker T., Richmond T.A., Mascher M., Steuernagel B., Scholz U., Ariyadasa R., Spannagl M., Nussbaumer T., Mayer K.F., Taudien S., Platzer M., Jeddeloh J.A., Springer N.M., Muehlbauer G.J., SteinN. (2013): Distribution, functional impact and origin mechanisms of copy number variation in the barley genome. Genome Biology, 14: R58.
Go to original source...
Go to PubMed...
- Newcomb R.D., Crowhurst R.N., Gleave A.P., Rikkerink E.A.H., Allan A.C., Beuning L.L., Bowen J.H., Gera E., Jamieson K.R., Janssen B.J., Laing W.A., McCartney S., Nain B., Ross G.S., Snowden K.C., Soulyere E.J.F. Walton E.F., Yauk Y.-K. (2006): Analyses of expressed sequence tags from apple. Plant Physiology, 141: 147-166.
Go to original source...
Go to PubMed...
- Ozsolak F., Ting D.T., Wittner S.B., Branningan B.W., Suchismita P., Bardeesy N., Ramaswamy S., Milos P.M., Haber D.A. (2010): Amplification-free digital gene expression profiling from minute cell quantities. Nature Methods, 7: 619-621.
Go to original source...
Go to PubMed...
- Patocchi A., Vinatzer B.A., Gianfranceschi L., Tartarini S., Zhang H.B., Sansavini S., Gessler C. (1999): Construction of a 550Kb BAC contig spanning the genomic region containing the apple scab resistance gene Vf. Molecular and General Genetics, 262: 884-891.
Go to original source...
Go to PubMed...
- Patocchi A., Fernandez-Fernandez F., Evans K., Gobbin D., Rezzonico F., Boudichevskaia A., Dunemann F.A., Stankiewicz-Kosyl M., Mathis-Jeanneteau F., Durel C.E., Gianfranceschi L., Costa F., Toller C., Cova V., Mott D., Komjanc M., Barbaro E., Kodde L., Rikkerink E., Gessler C., van de Weg E. (2009): Development and test of 21 multiplex PCRs composed of SSR spanning most of the apple genome. Tree Genetics and Genomes, 5: 211-223.
Go to original source...
- Russell J., Hackett C., Hedley P., Liu H., Milne L., Bayer M., Marshall D., Jorgensen L., Gordon S., Brennan R. (2014): The use of genotyping by sequencing in black currant (Ribes nigrum): developing high-resolution linkage maps in species without reference genome sequences. Molecular Breeding, 33: 835-849.
Go to original source...
- Sanger F., Coulson A.R. (1975): A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. Journal of Molecular Biology, 94: 441-448.
Go to original source...
Go to PubMed...
- Sanger F., Nicklen S., Coulson A.R. (1977): DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Science, 74: 5463-5467.
Go to original source...
Go to PubMed...
- Shokralla S., Spall J.L., Gibson J.F., Hajibabaei M. (2012): Next-generation sequencing technologies for environmental DNA research. Molecular Ecology, 21: 1794-1805.
Go to original source...
Go to PubMed...
- Shulaev V., Sargent D.J., Crowhurst R.N., Mockler T.C., Folkerts O., Delcher A.L., et al. (2011): The genome of woodland strawberry (Fragaria vesca). Nature Genetics, 43: 109-116.
Go to original source...
Go to PubMed...
- Soglio V., Costa F., Molthoff J.W., Mieke W., Weemen-Hendriks J., Schouten H.J., Gianfranceschi L. (2009): Transcription analysis of apple fruit development using cDNA microarrays. Tree Genetics and Genomes, 5: 685-698.
Go to original source...
- S oria-Guerra R.E., Rosales -Mendoz a S., Gasic K., Wiśniewski M., Band M., Korban S.S. (2011): Gene expression is highly regulated in early developing fruit of apple. Plant Molecular Biology and Reposiotory, 29: 885-897.
Go to original source...
- Stryer L., Augustyniak J., Michejda J. (2003): Biochemia. Warszawa, Wydawnictwo Naukowe PWN. (in Polish)
- Sun R., Chagne Y., Yang F., Wang Y., Li H., Zhao Y., Chen D., Wu T., Zhang X., Han Z. (2015): A dense SNP genetic map constructed using restriction site associated DNA sequencing enables detection of QTLs controlling apple fruit quality. BMC Genomics, 16: 747.
Go to original source...
Go to PubMed...
- Troggio M., Gleave A., Salvi S., Chagne D., Cestaro A., Kumar S., Crowhurst R.N., Gardiner S. (2012): Apple from genome to breeding. Tree Genetics and Genomes, 8: 509-529.
Go to original source...
- Velasco R., Zharkikh A., Affourtit J., Dhingra A., Cestaro A., Kalyanaraman A., Fontana P., Bhatnagar S.K., Troggio M., et al. (2010): The genome of the domesticated apple (Malus × domestica Borkh.). Nature Genetics, 42: 833-841.
Go to original source...
Go to PubMed...
- Wang Z., Gerstein M., Snyder M. (2009): RNA-seq: a revolutionary tool for transcriptomics. Nature Review Genetics, 10: 57-63.
Go to original source...
Go to PubMed...
- Ward J.A., Ponnala L., Weber C.A. (2012): Strategies for transcriptome analysis in nonmodel plants. American Journal of Botany, 99: 267-276.
Go to original source...
Go to PubMed...
- Watson J.D., Crick F.H.C. (1953): Genetical implications of the structure of deoxyribonucleic acid. Nature, 171: 964-967.
Go to original source...
Go to PubMed...
- Wullschleger S.D., Difazio S.P. (2003): Emerging use of gene expression microarrays in plant physiology. Comparative and Functional Genomics, 4: 216-224.
Go to original source...
Go to PubMed...
- Xu Y., Feng S., Jiao Q., Liu C., Zhang W., Chen W., Chen X. (2012): Comparison ofMdMYB1 sequences and expression of anthocyanin biosynthetic and regulatory genes between Malus domestica Borkh. cultivar 'Ralls' and its blushed sport. Euphytica, 185: 157-170.
Go to original source...
- Yang S., Fresnedo-Ramirez J., Wang M., Cote L., Schweitzer P., Barba P., Takacs E.M., Clark M., Luby J., Manns D.C., Sacks G., Mansfield A.K., Londo J., Fennel A., Gadoury D., Reisch B., Cadle-Davidson L., Sun O. (2016): A next generation marker genotyping platform, (AmpSeq) in heterozygous crops: a case study for marker-assisted selection in grapevine. Horticulture Research, 3: 16002.
Go to original source...
Go to PubMed...
- Zhang S., Chen W., Xin L., Gao Z., Hou Y., Yu X., Zhang Z., Qu S. (2014): Genomic variants of genes associated with three horticultural traits in apple revealed by genome re-sequencing. Horticulture Research, 1: 14045.
Go to original source...
Go to PubMed...
- Zhao T., Liang D., Wang P., Liu J., Ma F. (2012): Genome wide analysis and expression profiling of the DREB transcription factor gene family in Malus under abiotic stress. Molecular Genetics and Genomics, 287: 423-436.
Go to original source...
Go to PubMed...
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.