Czech Journal of Genetics and Plant Breeding, 2018 (vol. 54), issue 3

Detection of QTLs for cold tolerance at the booting stage in near-isogenic lines derived from rice landrace LijiangxintuanheiguOriginal Paper

Shu Ming YANG, Su Hua ZHANG, Tao YANG, Li WANG

Czech J. Genet. Plant Breed., 2018, 54(3):93-100 | DOI: 10.17221/98/2017-CJGPB  

Chilling damage significantly reduces grain yield in rice, while exploring major quantitative trait loci (QTLs) has the potential to improve rice production. Mapping of QTLs for 5 cold tolerance-related traits at the booting stage was conducted with SSR markers and inclusive composite interval mapping (ICIM) approach, based on 105 near-isogenic lines derived from a backcross between Lijiangxintuanheigu (LTH, cold-tolerant landrace) and Towada (cold-sensitive cultivar). Phenotype values were investigated under five cold-stress environments and analysed by the best linear unbiased prediction (BLUP). Twenty-one QTLs were identified on chromosomes 1, 2,...

Introgression of submergence tolerance into CO 43, a popular rice variety of India, through marker-assisted backcross breedingOriginal Paper

Hifzur RAHMAN, Vijayalakshmi DAKSHINAMURTHI, Sasikala RAMASAMY, Sudha MANICKAM, Ashok Kumar KALIYAPERUMAL, Suchismita RAHA, Naresh PANNEERSELVAM, Valarmathi RAMANATHAN, Jagadeeshselvam NALLATHAMBI, Robin SABARIAPPAN, Muthurajan RAVEENDRAN

Czech J. Genet. Plant Breed., 2018, 54(3):101-108 | DOI: 10.17221/149/2017-CJGPB  

Submergence is a major threat in rice growing areas of South and South-East Asia. Identification of a major QTL, Sub1 and advancements in marker-assisted backcrossing strategy enabled breeders to develop submergence tolerant versions of popular rice cultivars. In the present study, a marker-assisted backcross breeding (MABB) approach was used to introgress the Sub1 locus from the tolerant FR13A variety into CO 43, one of the popular rice varieties of southern India. Evaluation of early generation segregating progenies derived from the cross of CO 43 and FR13A revealed the introgression of Sub1, leading to increased tolerance...

Genome-wide association study for anther length in some elite bread wheat germplasmOriginal Paper

Xiaopeng SONG, Jie FENG, Zixia CUI, Chuanliang ZHANG, Daojie SUN

Czech J. Genet. Plant Breed., 2018, 54(3):109-114 | DOI: 10.17221/70/2017-CJGPB  

The anther is a crucial organ for the development of the spike in bread wheat (Triticum aestivum L.). Long anthers contain large amounts of pollen grains; thus, they are favourable for cross-pollination and increase resilience against adverse environmental conditions. We conducted a genome-wide association study (GWAS) of anther length in 305 elite wheat lines evaluated during 2013-2015 in two locations and two growing seasons. The mapping panel was genotyped using a high-density Illumina iSelect 90K single nucleotide polymorphism (SNP) array. The GWAS used 18763 SNPs and identified 17 markers associated with anther length in wheat. The loci...

Construction of a genetic linkage map of cigar tobacco (Nicotiana tabacum L.) based on SSR markers and comparative studiesOriginal Paper

Zhijun TONG, Bingguang XIAO, Xuejun CHEN, Dunhuang FANG, Yihan ZHANG, Changjun HUANG, Yongping LI

Czech J. Genet. Plant Breed., 2018, 54(3):115-122 | DOI: 10.17221/121/2017-CJGPB  

Genetic linkage maps representing the tobacco genome have been an important tool for breeding programs because of the elucidation of polygenic traits. We constructed a genetic linkage map of cigar tobacco (Nicotiana tabacum L.) based on an inter-type backcross population of 213 individuals and performed a comparative analysis with other published maps of dark tobacco and flue-cured tobacco. The map consisted of 562 SSR loci distributed on 24 tentative linkage groups and spanned a total length of 1341.18 cM with an average distance of 2.39 cM between adjacent markers. The comparative analysis revealed a Spearman correlation index of 0.93 for...

Genotypic variation in pre- and post-anthesis dry matter remobilization in Iranian wheat cultivars: associations with stem characters and grain yieldOriginal Paper

Mehdi JOUDI, Wim VAN DEN ENDE

Czech J. Genet. Plant Breed., 2018, 54(3):123-134 | DOI: 10.17221/93/2017-CJGPB  

The amount of carbohydrate accumulation and remobilization in the wheat stem can be estimated by monitoring changes in stem dry matter. Eighty-one wheat cultivars were examined in the Moghan region of Iran during the 2010-2011 and 2013-2014 growing seasons. Pre- and post-anthesis dry matter remobilization was quantified in the tested cultivars and their associations with stem characteristics and grain yield were investigated. There was substantial variation in stem length, weight, and specific weight among the tested cultivars. The majority of the cultivars did not show pre-anthesis dry matter remobilization in the stem or internodes. In contrast,...

Variation in ploidy level and morphological traits in the progeny of the triploid apple variety JonagoldOriginal Paper

Ping HE, Linguang LI, Lailiang CHENG, Haibo WANG, Yuansheng CHANG

Czech J. Genet. Plant Breed., 2018, 54(3):135-142 | DOI: 10.17221/201/2016-CJGPB  

Variation with respect to both ploidy level and morphology was characterized for a set of 690 seedlings of the triploid apple variety Jonagold, of which 481 were obtained via in vitro culture of mature embryos, and 209 via conventional germination. Their ploidy level was determined by a combination of flow cytometry and root tip chromosome counting. The assessed morphological traits were leaf length, width and shape, stomatal density, guard cell length and chloroplast width and number. A total of 452 seedlings were aneuploid, 225 diploid, nine triploid and four tetraploid. All four tetraploid seedlings were derived by in vitro culture....

Reaction of Nicotiana species and cultivars of tobacco to Tobacco mosaic virus and detection of the N gene that confers hypersensitive resistanceShort Communication

Anna DEPTA, Karolina KURSA, Teresa DOROSZEWSKA, Dorota LASKOWSKA, Anna TROJAK-GOLUCH

Czech J. Genet. Plant Breed., 2018, 54(3):143-146 | DOI: 10.17221/81/2017-CJGPB  

Tobacco mosaic virus (TMV) brings increasing losses in the cultivation of tobacco. Sixty-two cultivars of tobacco and eleven species of Nicotiana were evaluated for resistance to TMV. Biological tests at two temperature ranges, DAS-ELISA and molecular markers were applied to assess the resistance to TMV. Most cultivars of tobacco showed susceptibility (S) to TMV, two were tolerant (T), while others revealed a hypersensitive response (HR). Hypersensitivity, determined by the N gene, occurred only at a temperature below 22°C. At a temperature above 28°C, all the cultivars showed mosaic discolorations or extensive necrosis....