Czech J. Genet. Plant Breed., 2018, 54(3):135-142 | DOI: 10.17221/201/2016-CJGPB

Variation in ploidy level and morphological traits in the progeny of the triploid apple variety JonagoldOriginal Paper

Ping HE*, Linguang LI*, Lailiang CHENG, Haibo WANG, Yuansheng CHANG
Shandong Institute of Pomology, Taian, Shandong, P.R. China

Variation with respect to both ploidy level and morphology was characterized for a set of 690 seedlings of the triploid apple variety Jonagold, of which 481 were obtained via in vitro culture of mature embryos, and 209 via conventional germination. Their ploidy level was determined by a combination of flow cytometry and root tip chromosome counting. The assessed morphological traits were leaf length, width and shape, stomatal density, guard cell length and chloroplast width and number. A total of 452 seedlings were aneuploid, 225 diploid, nine triploid and four tetraploid. All four tetraploid seedlings were derived by in vitro culture. When the triploid seedlings were genotyped at the S-locus and at selected microsatellite loci, we found that the Jonagold stigma was compatible with pollen which shared some of the maternal parent S-locus alleles.

Keywords: flow cytometry; karyotypic analysis; S-alleles; SSR markers

Published: September 30, 2018  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Ping H, Linguang L, CHENG L, WANG H, CHANG Y. Variation in ploidy level and morphological traits in the progeny of the triploid apple variety Jonagold. Czech J. Genet. Plant Breed. 2018;54(3):135-142. doi: 10.17221/201/2016-CJGPB.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Basssam B.J., Caetano-Anolle G., Gresshoff P.M. (1991): Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry, 196: 80-83. Go to original source... Go to PubMed...
    2. Bingham E.T. (1968): Stomata chloroplasts in alfalfa at four ploidy levels. Crop Science, 8: 509-510. Go to original source...
    3. Einset J. (1952): Spontaneous polyploidy in cultivated apples. Proceedings of the American Society for Horticaltural Science, 59: 291-302.
    4. Jackson S., Chen Z.J. (2010): Genomic and expression plasticity of polyploidy. Current Opinion in Plant Biology, 13: 153-157. Go to original source... Go to PubMed...
    5. Jiao Y., Wickett N.J., Ayyampalayam S., Chanderbali A.S., Landherr L. (2011): Ancestral polyploidy in seed plants and angiosperms. Nature, 473: 97-100. Go to original source... Go to PubMed...
    6. Koller B., Tenzer I., Gessler C. (2000): SSR analysis of apple scab lesions. Integrated Control of Pome Fruit Diseases. IOBC/WPRS Bulletin, 23: 93-98.
    7. Kondo K., Lavaral P.S. (1984): A cytotaxonomic study of some Australian species of Drosera L. (Droseraceae). Botanical Journal of the Linnean Society, 88: 317-333. Go to original source...
    8. Laubscher F.X., Hurter N. (1960): The chromosome numbers of seedling progeny from triploid apples. South African Journal of Science, 3: 31-39.
    9. Lespinasse Y., Alston F.H., Watkins R. (1976): Cytological techniques for use in apple breeding. Annals of Applied Biology, 82: 349-353. Go to original source...
    10. Levin D. (2002): The Role of Chromosomal Change in Plant Evolution. New York, Oxford University Press. Go to original source...
    11. Michaelson M.J., Price H.J., Johnson J.S., Ellison J.R. (1991): Variation of nuclear DNA content in Helianthus anuus (Asteraccae). American Journal of Botany, 78: 1238-1243. Go to original source...
    12. Minamikawa M.F., Koyano R., Kikuchi S., Koba T., Sassa H. (2014): Identification of SFBB-containing canonical and noncanonical SCF complexes in pollen of apple (Malus × domestica). PLoS ONE, 9: e97642. Go to original source... Go to PubMed...
    13. Moehizuki A., Sueoka N. (1955): Genetic studies on the number of plastid in atomata and effects of autopoly ploidy in sugarbeets. Cytologia (Tokyo), 20: 358-366. Go to original source...
    14. Murashige T., Skoog F. (1962) A revised medium for rapid growth and bioassays wih tobacco tissue cultures. Plant Physiology, 15: 473-497. Go to original source...
    15. Najcevaka C.M., Speckmann G.J. (1968): Number of chloroplast and pollen grain pores in diploid and tetraploid varieties of some Trifolium species. Euphytica, 17: 357-362. Go to original source...
    16. Passvetaeva E.G. (1985): Identification of stone fruit cultivars and their hybrids that forming unreduced pollen. Plant Breeding Abstracts, 55: 8910-8912.
    17. Rensing S.A. (2014): Gene duplication as a driver of plant morphogenetic evolution. Plant Biology, 17: 43-48. Go to original source... Go to PubMed...
    18. Riccardi C., Nicoletti I. (2006): Analysis of apoptosis by propidium iodide staining and flow cytometry. Nature Protocols, 1: 1458-1461. Go to original source... Go to PubMed...
    19. Sanford J.C. (1983): Ploidy manipulation. In: Methods in Fruit Breeding. West Lafayette, Purdue University Press: 100-123.
    20. Soltis D., Soltis P., Tate J. (2003): Advances in the study of polyploidy since plant speciation. New Phytologist, 161: 173-191. Go to original source...
    21. Soltis P.S., Marchant D.B., Van de Peer Y., Soltis D.E. (2015): Polyploidy and genome evolution in plants. Genetics and Development, 35: 119-125. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content