Czech J. Genet. Plant Breed., 2018, 54(3):115-122 | DOI: 10.17221/121/2017-CJGPB

Construction of a genetic linkage map of cigar tobacco (Nicotiana tabacum L.) based on SSR markers and comparative studiesOriginal Paper

Zhijun TONG, Bingguang XIAO*, Xuejun CHEN, Dunhuang FANG, Yihan ZHANG, Changjun HUANG, Yongping LI
Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, P.R. China

Genetic linkage maps representing the tobacco genome have been an important tool for breeding programs because of the elucidation of polygenic traits. We constructed a genetic linkage map of cigar tobacco (Nicotiana tabacum L.) based on an inter-type backcross population of 213 individuals and performed a comparative analysis with other published maps of dark tobacco and flue-cured tobacco. The map consisted of 562 SSR loci distributed on 24 tentative linkage groups and spanned a total length of 1341.18 cM with an average distance of 2.39 cM between adjacent markers. The comparative analysis revealed a Spearman correlation index of 0.93 for marker order conservation with the previously published maps constructed for different tobacco types. Approximately 91% of the SSR markers common to other inter-type maps were located in the same positions as in previous maps. The three maps exhibit good synteny in terms of the shared markers, which suggests that there might be no translocation variations between the genomes of the cigar, dark and flue-cured tobaccos. These results indicate the feasibility of generating a unique genetic map of preferred traits in cigar tobacco and that such mapping may be helpful for breeding programs because plants derived from different inter-type populations can be rapidly scanned using the markers associated with useful cigar traits

Keywords: common tobacco; comparative mapping; genetic map; microsatellite marker

Published: September 30, 2018  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
TONG Z, XIAO B, CHEN X, FANG D, ZHANG Y, HUANG C, Yongping L. Construction of a genetic linkage map of cigar tobacco (Nicotiana tabacum L.) based on SSR markers and comparative studies. Czech J. Genet. Plant Breed. 2018;54(3):115-122. doi: 10.17221/121/2017-CJGPB.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files:

    Download fileTong_ESM.pdf

    File size: 6.07 MB

    References

    1. Bindler G., van der Hoeven R., Gunduz I., Plieske J., Ganal M., Rossi L., Gadani F., Donini P. (2007): A microsatellite marker based linkage map of tobacco. Theoretical and Applied Genetics, 114: 341-349. Go to original source... Go to PubMed...
    2. Bindler G., Plieske J., Bakaher N., Gunduz I., Ivanov N., Van der Hoeven R., Ganal M.P. (2011): A high density genetic map of tobacco (Nicotiana tabacum L.) obtained from large scale microsatellite marker development. Theoretical and Applied Genetics, 123: 219-230. Go to original source... Go to PubMed...
    3. Bombarely A., Rosli H.G., Vrebalov J., Moffett P., Mueller L., Martin G. (2012): A draft genome sequence of Nicotiana benthamiana to enhance molecular plant-microbe biology research. Molecular Plant-Microbe Interactions, 25: 1523-1530. Go to original source... Go to PubMed...
    4. Bourke P.M., Voorrips R.E., Visser R.G.F., Maliepaard C. (2015): The double-reduction landscape in tetraploid potato as revealed by a high-density linkage map. Genetics, 201: 853-863. Go to original source... Go to PubMed...
    5. Bratteler M., Lexer C., Widmer A. (2006): A genetic linkage map of Silene vulgaris based on AFLP markars. Gemome, 49: 320-327. Go to original source... Go to PubMed...
    6. Cai C.C., Chai L.G., Wang Y., Xu F.S., Zhang J.J., Lin G.P. (2009): Construction of genetic linkage map of burley tobacco (Nicotiana tabacum L.) and genetic dissection of partial traits. Acta Agronomica Sinica, 35: 1646-1654. Go to original source...
    7. Doğanlar S., Frary A., Daunay M.C., Huvenaars K., Mank R., Frary A. (2014): High resolution map of eggplant (Solanum melongena) reveals extensive chromosome rearrangement in domesticated members of the Solanaceae. Euphytica, 198: 231-241. Go to original source...
    8. Edwards K.D., Fernandez-Pozo N., Drake-Stowe K., Humphry M., Evans A.D., Bombarely A., Allen1 F., Hurst R., White B., Kernodle S.P., Bromley J.R., Sanchez-Tamburrino J.P., Lewis R.S., Mueller L.A. (2017): A reference genome for Nicotiana tabacum enables map-based cloning of homeologous loci implicated in nitrogen utilization efficiency. BMC Genomics, 18: 448. Go to original source... Go to PubMed...
    9. Gerstel D.U., Burns J.A., Burk L.G. (1979): Cytoplasmic male sterility in Nicotiana, restoration of fertility, and the nucleolus. Genetics, 89: 157-169. Go to original source... Go to PubMed...
    10. Gerstel D.U., Burns J.A., Sand S.A. (1980): Cytogenetics of flower modification of a cytoplasmic male-sterile tobacco. Genetics, 96: 223-235. Go to original source... Go to PubMed...
    11. Gong D.P., Huang L., Xu X.H., Wang C.Y., Ren M., Wang C.K., Chen M.L. (2016): Construction of a high-density SNP genetic map in flue-cured tobacco based on SLAFseq. Molecular Breeding, 36: 100. Go to original source...
    12. Goodspeed T.H. (1954): The Genus Nicotiana. Waltham, Chronica Botanica Company.
    13. Lu X.P., Gui Y.J., Xiao B.G., Li Y.P., Tong Z.J., Liu Y., Bai X.F., Wu W.R., Xia L., Huttner E. (2013): Development of DArT markers for a linkage map of flue-cured tobacco. Chinese Science Bulletin, 58: 641-648. Go to original source...
    14. Lyttle T.W. (1991): Segregation distorters. Annual Review of Genetics, 25: 511-557. Go to original source... Go to PubMed...
    15. Mantel N. (1967): The detection of disease clustering and a generalized regression approach. Cancer Research, 27: 209-220.
    16. Roder M.S., Korzun V., Wendehake K., Plaschke J., Tixer M.H. (1998): A microsatellite map of wheat. Genetics, 149: 2007-2023. Go to original source... Go to PubMed...
    17. Rohlf F.J. (1989): NTSYS-PC: Numerical Taxonomy and Multivariate Analysis System. New York, Exeter Publisher.
    18. Shirasawa K., Isobe S., Hirakawa H., Asamizu E., Fukuoka H., Just D., Rothan C., Sasamoto S., Fujishiro T., Kishida Y. (2010): SNP discovery and linkage map construction in cultivated tomato. DNA Research, 17: 381-391. Go to original source... Go to PubMed...
    19. Sierro N., van Oeveren J., van Eijk M.J., Martin F., Stormo K.E., Peitsch M.C., Ivanov N.V. (2013): Whole genome profiling physical map and ancestral annotation of tobacco Hicks Broadleaf. Plant Journal, 75: 880-889. Go to original source... Go to PubMed...
    20. Sierro N., Battey J.N., Ouadi S., Bakaher N., Bovet L., Willig A., Goepfert S., Peitsch M.C., Ivanov N.V. (2014): The tobacco genome sequence and its comparison with those of tomato and potato. Nature Communication, 5: 3833. Go to original source... Go to PubMed...
    21. Tadmor Y., Zamir D., Ladizinsky G. (1987): Genetic mapping of an ancient translocation in the genus Lens. Theoretical and Applied Genetics, 73: 883-892. Go to original source... Go to PubMed...
    22. Tong Z.J., Yang Z.M., Chen X.J., Jiao F.C., Li X.Y., Wu X.F., Gao Y.L., Xiao B.G., Wu W.R. (2012): Large-scale development of microsatellite markers in Nicotiana tabacum and construction of a genetic map of flue-cured tobacco. Plant Breeding, 131: 674-680. Go to original source...
    23. Tong Z.J., Xiao B.G., Jiao F.C., Fang D.H., Zeng J.M., Wu X.F., Chen X.J., Yang J.K., Li Y.P. (2016): Large-scale development of SSR markers in tobacco and construction of a linkage map in flue-cured tobacco. Breeding Science, 66: 381-390. Go to original source... Go to PubMed...
    24. Van Ooijen J.W. (2006): JoinMap ® 4.0. Software for the Calculation of Genetic Linkage Maps in Experimental Populations. Wageningen, Kyazma B.V.
    25. Vontimitta V., Lewis R.S. (2012): Mapping of quantitative trait loci affecting resistance to Phytophthora nicotianae in tobacco (Nicotiana tabacum L.) line Beinhart-1000. Molecular Breeding, 29: 89-98. Go to original source...
    26. Voorrips R.E. (2002): MapChart: software for the graphical presentation of linkage maps and QTLs. Journal of Heredity, 93: 77-78. Go to original source... Go to PubMed...
    27. Wernsman E.A. (1987): Tobacco. New York, Macmillan.
    28. Xue S., Zhang Z., Lin F., Kong Z., Cao Y. (2008): A highdensity intervarietal map of the wheat genome enriched with markers derived from expressed sequence tags. Theoretical and Applied Genetics, 117: 181-189. Go to original source... Go to PubMed...
    29. Zhang X.F., Sun H.H., Xu Y., Chen B., Yu S.C., Geng S.S., Wang Q. (2016): Development of a large number of SSR and InDel markers and construction of a high-density genetic map based on a RIL population of pepper (Capsicum annuum L.). Molecular Breeding, 36: 92. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content