Czech Journal of Genetics and Plant Breeding, 2018 (vol. 54), issue 2
The progress of genetic improvement in alfalfa (Medicago sativa L.)Review
Tanweer KUMAR, Ai-Ke BAO, Zhulatai BAO, Fei WANG, Li GAO, Suo-Min WANG
Czech J. Genet. Plant Breed., 2018, 54(2):41-51 | DOI: 10.17221/46/2017-CJGPB 
Alfalfa (Medicago sativa L.) is a perennial and outcrossing species, widely grown as a forage legume for hay, pasture and silage. The genetic engineering approaches involve the transfer of useful or novel gene(s) into alfalfa to improve desired traits. The recent development of genetic engineering is extensively applied to basic and applied research for alfalfa improvement, including improvement of herbicide resistance, reinforcement of the resistance to biotic and abiotic stresses, production of novel compounds, improved yield for industrial and/or pharmaceutical proteins and renewable energy sources. On the other hand, alfalfa forage needs...
Quantitative trait loci conferring grain selenium nutrient in durum wheat × wild emmer wheat RIL populationOriginal Paper
Jun YAN, Wen-tao XUE, Rong-zhi YANG, Hai-bo QIN, Gang ZHAO, Fahima TZION, Jian-ping CHENG
Czech J. Genet. Plant Breed., 2018, 54(2):52-58 | DOI: 10.17221/112/2016-CJGPB
The genetic and phenotypic basis of grain selenium concentration (GSeC) and yield per plant (GSeY) was studied in a tetraploid wheat population consisting of 152 F6 recombinant inbred lines (RILs) derived from a cross between Triticum dicoccoides (accession G18-16) and durum wheat cultivar Langdon (LDN) grown under three different environments over 2 years. Wide genetic variation was found among RILs for GSeC and GSeY. A total of 15 QTL effects on 9 chromosomes associated with GSeC and GSeY were detected, with a logarithm od the odds (LOD) score ranging from ca. 3.2 to 11.7, explaining 1.4%...
Mapping QTLs for cold tolerance at seedling stage using an Oryza sativa × O. rufipogon backcross inbred line populationOriginal Paper
Shouwu YU, Meizhen LI, Yeqing XIAO, Derun HUANG, Dazhou CHEN
Czech J. Genet. Plant Breed., 2018, 54(2):59-64 | DOI: 10.17221/154/2016-CJGPB
Tolerance to low temperature is an important factor affecting the growth and development of rice (Oryza sativa L.) at an early growing season in the temperate region, and at high altitudes of tropical regions. In this study, a backcross inbred line (BIL) population derived from an interspecific cross between Xieqingzao B (O. sativa L.) and an accession of Dongxiang wild rice (O. rufipogon Griff.) was used to identify quantitative trait loci (QTLs) associated with cold tolerance at the seedling stage. Seedlings were treated with a temperature of 6°C for 2 days and seedling mortality was measured for QTL mapping....
Molecular mapping of leaf rust resistance gene LrL224 in Chinese wheat cultivar L224-3Original Paper
Yue ZHOU, Huan LI, Pei-pei ZHANG, Lin WANG, Zai-feng LI
Czech J. Genet. Plant Breed., 2018, 54(2):65-70 | DOI: 10.17221/19/2017-CJGPB
Leaf rust, caused by Puccinia triticina, is a major wheat disease worldwide. The chinese wheat cultivar L224-3 showed high resistance to most of P. triticina pathotypes in the seedling and adult stage. Identifying and mapping the leaf rust resistance gene(s) in L224-3 is very useful for breeding leaf rust resistant wheat cultivars. In the present study, the wheat cultivar L224-3 and thirty-six lines with known leaf rust resistance genes were inoculated with 15 pathotypes at the seedling stage for gene postulation. A total of 144 F2:3 lines from the cross L224-3 × Zhengzhou 5389 were...
Assessment of gene action and combining ability for fibre and yield contributing trais in interspecific and intraspecific hybrids of cottonOriginal Paper
Sana MUNIR, Muhammad Kamran QURESHI, Ahmad Naeem SHAHZAD, Hamid MANZOOR, Muhammad Ahmad SHAHZAD, Kashif ASLAM, Habib-Ur-Rehman ATHAR
Czech J. Genet. Plant Breed., 2018, 54(2):71-77 | DOI: 10.17221/54/2017-CJGPB
Combining ability analysis was performed in order to identify high-yielding genotypes and hybrids. Six lines were crossed with three testers using a line × tester mating design. General combining ability (GCA) of parents, specific combining ability (SCA) of hybrids and gene action for several yield components and fibre were studied in the experiment. Additive gene action was observed in most traits except bolls/plant, sympodia/plant, monopodia/plant, total nodes, seed index, lint/seed, staple strength and seed cotton yield/plant which were influenced by non-additive gene action. GCA revealed that Giza-7 was a good general combiner for staple length,...
Evaluation of pear (Pyrus communis L.) hybrid combinations for the transmission of fire blight resistance and fruit characteristicsOriginal Paper
Yasemin EVRENOSOGLU, Kerem MERTOGLU
Czech J. Genet. Plant Breed., 2018, 54(2):78-85 | DOI: 10.17221/17/2017-CJGPB
Fire blight is one of the most destructive diseases of pome fruits, especially pears. In current conditions when the demand for organic products is increasing, improvement of resistant rootstock and varieties is becoming important due to the lack of an effective disease management method against fire blight caused by Erwinia amy-lovora as well as the harmful effects of chemicals on environment, human and animal health. The findings of the researchers as to which variety performs well in terms of the transmission of fire blight resistance are quite important for new breeding programs. In the study, three varieties with high commercial value...
Evaluation of genetic variability within actual hop (Humulus lupulus L.) cultivars by an enlarged set of molecular markersShort Communication
Josef PATZAK, Alena HENYCHOVÁ
Czech J. Genet. Plant Breed., 2018, 54(2):86-91 | DOI: 10.17221/175/2016-CJGPB
Traditional hop (Humulus lupulus L.) cultivars have been used in the brewing industry for a long time. Globally, about ten new breeding lines were released to the market in each decade from ~1970 to 1999. Since 2006, the rate of release of new cultivars has increased tenfold. It is, therefore, important to identify their genotype and origin. Molecular genetic methods based on DNA are the most appropriate technology for this purpose. Recently, we developed an efficient marker system for the authenticity control of hop genotypes based on expressed sequence tag-simple sequence repeats (EST-SSR). In the present study, we enlarged the previously...