Czech J. Genet. Plant Breed., 2018, 54(2):86-91 | DOI: 10.17221/175/2016-CJGPB

Evaluation of genetic variability within actual hop (Humulus lupulus L.) cultivars by an enlarged set of molecular markersShort Communication

Josef PATZAK*, Alena HENYCHOVÁ
Hop Research Institute Co., Ltd., Žatec, Czech Republic

Traditional hop (Humulus lupulus L.) cultivars have been used in the brewing industry for a long time. Globally, about ten new breeding lines were released to the market in each decade from ~1970 to 1999. Since 2006, the rate of release of new cultivars has increased tenfold. It is, therefore, important to identify their genotype and origin. Molecular genetic methods based on DNA are the most appropriate technology for this purpose. Recently, we developed an efficient marker system for the authenticity control of hop genotypes based on expressed sequence tag-simple sequence repeats (EST-SSR). In the present study, we enlarged the previously established EST-SSR set with 27 new polymorphic markers and evaluated molecular genetic variability within 135 traditional and new world hop cultivars. Two sets of 10 markers effectively differentiated all used cultivars, with the exception of cultivars derived from the same original genotype such as Saaz, Spalt, Tettnang and Nadwislawsky. Results of molecular genetic variability analyses corresponded with the genealogical and geographical origin of the key cultivars.

Keywords: expressed sequence tag-simple sequence repeat (EST-SSR); cluster analysis; principal coordinate analysis

Published: June 30, 2018  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
PATZAK J, HENYCHOVÁ A. Evaluation of genetic variability within actual hop (Humulus lupulus L.) cultivars by an enlarged set of molecular markers. Czech J. Genet. Plant Breed. 2018;54(2):86-91. doi: 10.17221/175/2016-CJGPB.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files:

    Download file175-2016 Patzak ESM.pdf

    File size: 296.25 kB

    References

    1. Barth-Haas Group (2013): Barth-Haas Hops Companion: A Guide to the Varieties of Hops and Hop Products. Kostelecky T. (ed.). 2nd Ed. Yakima, John I. Haas Incorporated.
    2. Bassil N.V., Gilmore B., Oliphant J.M., Hummer K.E., Henning J.A. (2008): Genic SSRs for European and North American hop (Humulus lupulus L.). Genetic Resources and Crop Evolution, 55: 959-969. Go to original source...
    3. Clark S.M., Vaitheeswaran V., Ambrose S.J., Purves R.W., Page J.E. (2013): Transcriptome analysis of bitter acid biosynthesis and precursor pathways in hop (Humulus lupulus). BMC Plant Biology, 13: 12. Go to original source... Go to PubMed...
    4. Dresel M., Vogt C., Dunkel A., Hofmann T. (2016): The bitter chemodiversity of hops (Humulus lupulus L.). Journal of Agricultural and Food Chemistry, 64: 7789-7799. Go to original source... Go to PubMed...
    5. Fujii H., Ogata T., Shimada T., Endo T., Iketani H., Shimizu H., Omura M. (2013): Minimal marker: an algorithm and computer program for the identification of minimal sets of discriminating DNA markers for efficient variety identification. Journal of Bioinformatics and Computational Biology, 11: 1-17. Go to original source... Go to PubMed...
    6. Henning J.A., Coggins J., Peterson M. (2015): Simple SNP-based minimal marker genotyping for Humulus lupulus L. identification and variety validation. BMC Research Notes, 8: 542. Go to original source... Go to PubMed...
    7. Howard E.L., Whittock S.P., Jakše J., Carling J., Matthews P.D., Probasco G., Henning J.A., Darby P., Čerenak A., Javornik B., Kilian A. Koutoulis A. (2011): High-throughput genotyping of hop (Humulus lupulus L.) utilising diversity arrays technology (DArT). Theoretical and Applied Genetics, 122: 1265-1280. Go to original source... Go to PubMed...
    8. Jakše J., Bandelj D., Javornik B. (2002): Eleven new microsatellites for hop (Humulus lupulus L.). Molecular Ecology Notes, 2: 544-546. Go to original source...
    9. Jakše J., Štajner N., Luthar Z., Jelsch J.M., Javornik B. (2011): Development of transcript-associated microsatellite markers for diversity and linkage mapping studies in hop (Humulus lupulus L.). Molecular Breeding, 28: 227-239. Go to original source...
    10. Koelling J., Coles M.C., Matthews P.D., Schwekendiek A. (2012): Development of new microsatellite markers (SSRs) for Humulus lupulus. Molecular Breeding, 30: 479-484. Go to original source...
    11. Krofta K., Patzak J. (2011): Investigation of Czech hop varieties authenticity by means of chemical and genetic analyses. Kvasný průmysl, 57: 296-304. Go to original source...
    12. Lemmens G.W.Ch. (2014): The Breeding and Parentage of Hop Varieties. Available at https://bsgcraftbrewing.com/Resources/CraftBrewing/PDFs/Agricultural_Reports_and_Papers/TheBreeding_Varieties.pdf
    13. Matthews P.D., Coles M.C., Pitra N.J. (2013): Next generation sequencing for a plant of great tradition: Application of NGS to SNP detection and validation in hops (Humulus lupulus L). Brewing Science, 66: 185-191.
    14. Nagel J., Culley L.K., Lu Y., Liu E., Matthews P.D., Stevens J.F., Page J.E. (2008): EST analysis of hop glandular trichomes identifies an O-methyltransferase that catalyzes the biosynthesis of xanthohumol. Plant Cell, 20: 186-200. Go to original source... Go to PubMed...
    15. Natsume S., Takagi H., Shiraishi A., Murata J., Toyonaga H., Patzak J., Takagi M., Yaegashi H., Uemura A., Mitsuoka C., Yoshida K., Krofta K., Satake H., Terauch R., Ono E. (2015): The draft genome of hop (Humulus lupulus), an essence for brewing. Plant Cell Physiology, 56: 428-441. Go to original source... Go to PubMed...
    16. Patzak J. (2001): Comparison of RAPD, STS, ISSR and AFLP molecular methods used for assessment of genetic diversity in hop (Humulus lupulus L.). Euphytica, 121: 9-18. Go to original source...
    17. Patzak J., Matoušek J. (2011): Development and evaluation of expressed sequence tag-derived microsatellite (EST-SSR) markers for genotyping of hop (Humulus lupulus L.). Biologia Plantarum, 55: 761-765. Go to original source...
    18. Patzak J., Matoušek J. (2013a): Combination of sets of primers for detection of genetic polymorphism of Czech hop species and determination thereof. Czech Utility Model, 25678, 18 July 2013.
    19. Patzak J., Matoušek J. (2013b): Methodology of Utilization of Molecular-genetic Markers of Gene Sequences and Genetic Elements in Breeding and Management of Hop (Humulus lupulus). Certified Methodology. Žatec, Hop Research Institute. (in Czech)
    20. Patzak J., Vrba L., Matoušek J. (2007): New STS molecular markers for assessment of genetic diversity and DNA fingerprinting in hop (Humulus lupulus L.). Genome, 50: 15-25. Go to original source... Go to PubMed...
    21. Patzak J., Nesvadba V., Krofta K., Henychová A., Marzoev A.I., Richards K. (2010): Evaluation of genetic variability of wild hops (Humulus lupulus L.) in Canada and Caucasus region by chemical and molecular methods. Genome, 53: 545-557. Go to original source... Go to PubMed...
    22. Raymond M., Rousset F. (1995): GENEPOP, version 1.2: population genetics software for exact tests and ecuminicism. Journal of Heredity, 86: 248-249. Go to original source...
    23. Seefelder S., Ehrmaier H., Schweizer G., Seigner E. (2000): Genetic diversity and phylogenetic relationships among accessions of hop, Humulus lupulus, as determined by amplified fragment length polymorphism fingerprinting compared with pedigree data. Plant Breeding, 119: 257-263. Go to original source...
    24. Singh S., Gupta S., Mani A., Chaturvedi A. (2012): Mining and gene ontology based annotation of SSR markers from expressed sequence tags of Humulus lupulus. Bioinformation, 8: 114-122. Go to original source... Go to PubMed...
    25. Štajner N., Jakše J., Kozjak P., Javornik B. (2005): The isolation and characterisation of microsatellites in hop (Humulus lupulus L.). Plant Science, 168: 213-221. Go to original source...
    26. Štajner N., Šatovič Z., Čerenak A., Javornik B. (2008): Genetic structure and differentiation in hop (Humulus lupulus L.) as inferred from microsatellites. Euphytica, 161: 301-311. Go to original source...
    27. Xu H., Zhang F., Liu B., Huhman D.V., Sumner L.W., Dixon R.A., Wang G. (2013): Characterization of the formation of branched short-chain fatty acid: CoAs for bitter acid biosynthesis in hop glandular trichomes. Molecular Plant, 6: 1301-1317. Go to original source... Go to PubMed...
    28. Yamauchi H., Mukouzaka Y., Taniguchi T., Nakashima K., Furukubo S., Harada M. (2014): Newly developed SNP-based identification method of hop cultivars. Journal of the American Society of Brewing Chemists, 72: 239-245. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content