Czech Journal of Genetics and Plant Breeding, 2017 (vol. 53), issue 4
INDEX OF VOLUME 53
editors
Czech J. Genet. Plant Breed., 2017, 53(4):I-IV | DOI: 10.17221/10243-CJGPB 
Tissue culture-based Agrobacterium-mediated and in planta transformation methodsReview
Mohsen NIAZIAN, Seyed Ahmad SADAT NOORI, Petr GALUSZKA, Seyed Mohammad Mahdi MORTAZAVIAN
Czech J. Genet. Plant Breed., 2017, 53(4):133-143 | DOI: 10.17221/177/2016-CJGPB
Gene transformation can be done in direct and indirect (Agrobacterium-mediated) ways. The most efficient method of gene transformation to date is Agrobacterium-mediated method. The main problem of Agrobacterium-method is that some plant species and mutant lines are recalcitrant to regeneration. Requirements for sterile conditions for plant regeneration are another problem of Agrobacterium-mediated transformation. Development of genotype-independent gene transformation method is of great interest in many plants. Some tissue culture-independent Agrobacterium-mediated gene transformation methods are reported...
Analysis of the relationship between Wx gene polymorphisms and amylose content in hulless barleyOriginal Paper
Kunlun WU, Xiaohua YAO, Youhua YAO, Dezhao CHI, Zongyun FENG
Czech J. Genet. Plant Breed., 2017, 53(4):144-152 | DOI: 10.17221/105/2016-CJGPB
I2-KI staining was used to phenotype 151 hulless barley plants, which determined that five samples were of the waxy variety, namely 14-Z152, IG107028, Puebla, Hu Zhu Shuang Cao Ren, and APM-HC1905. Using the dual-wavelength method, the average amylose content of the 151 samples was 25.9%, ranging from 4.9 to 38.5%. The average amylose content of the five waxy varieties was 14.3%, ranging from 4.9 to 18.6%. Genomic DNA from 48 samples showing a significantly variable amylose content was used as template and PCR amplified using primer pair P4. Statistical analysis indicated that the PCR product size positively correlated with amylose content....
Genetic analysis and fine mapping of the RK4 gene for round kernel in rice (Oryza sativa L.)Original Paper
Shengqiang LI, Ruiyue ZHANG, Jipeng CHEN, Jie ZOU, Tao LIU, Guohua ZHOU
Czech J. Genet. Plant Breed., 2017, 53(4):153-158 | DOI: 10.17221/172/2016-CJGPB
Grain shape of rice is an important trait for both yield and quality. A rice rk4 (round kernel) mutant was obtained from the japonica variety Zhonghua 11 by radiation of 60Co-γ. The grain width of the mutant was increased and the length was decreased. Simultaneously, the 1000-grain weight was slightly reduced, therefore the grain shape of the mutant tended to be small and round. In this study, genetic analysis and gene mapping of the mutant gene were carried out using the F2 and F3 populations derived from the mutant and the indica variety Xianhui 8006. The results suggested that the...
Linkage disequilibrium and association mapping of fibre quality traits in elite Asiatic cotton (Gossypium arboreum) germplasm populationsOriginal Paper
Khushboo SETHI, Priyanka SIWACH, Surender Kumar VERMA
Czech J. Genet. Plant Breed., 2017, 53(4):159-167 | DOI: 10.17221/142/2016-CJGPB
Cotton productivity has been hindered by the narrow genetic base of cultivated cotton. Linkage disequilibrium-based association mapping has become a powerful molecular tool to dissect and exploit genetic diversity. In the present study, population structure and marker-trait associations for fibre quality traits in genotypes belonging to six races of Gossypium arboreum were assessed. Out of 300 simple sequence repeat (SSR) markers, 100 were found polymorphic, yielding a total of 240 alleles (all polymorphic). Structure analysis revealed allelic admixtures between genotypes. A Q-matrix exhibited mixed ancestry for the majority of genotypes,...
SSR markers distinguish traditional Italian bean (Phaseolus vulgaris L.) landraces from LamonShort Communication
Francesca CARUCCI, Raffaele GARRAMONE, Riccardo AVERSANO, Domenico CARPUTO
Czech J. Genet. Plant Breed., 2017, 53(4):168-171 | DOI: 10.17221/91/2016-CJGPB
In this study, 12 microsatellite markers (SSR) were evaluated for their applicability to protect from frauds and misuse the Italian PGI product "Common bean from Lamon". SSR analysis generated polymorphic alleles, with an average of 4 alleles per locus and all in the range of molecular weight between 181 and 284 bp. Twenty-nine variety-specific fragments were identified, which might be reasonably adopted for characterization and traceability purposes. Cluster analysis well outlined the relationships between the genotypes studied. Overall, our study underlines the use and usefulness of molecular markers to protect both farmers and consumers from frauds.