Czech J. Genet. Plant Breed., 2017, 53(4):144-152 | DOI: 10.17221/105/2016-CJGPB
Analysis of the relationship between Wx gene polymorphisms and amylose content in hulless barleyOriginal Paper
- 1 Sichuan Agricultural University, Chengdu, P.R. China
- 2 State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai, P.R. China
- 3 Academy of Agricultural and Forestry Sciences of Qinghai University, Xining, Qinghai, P.R. China
- 4 Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, Qinghai, P.R. China
I2-KI staining was used to phenotype 151 hulless barley plants, which determined that five samples were of the waxy variety, namely 14-Z152, IG107028, Puebla, Hu Zhu Shuang Cao Ren, and APM-HC1905. Using the dual-wavelength method, the average amylose content of the 151 samples was 25.9%, ranging from 4.9 to 38.5%. The average amylose content of the five waxy varieties was 14.3%, ranging from 4.9 to 18.6%. Genomic DNA from 48 samples showing a significantly variable amylose content was used as template and PCR amplified using primer pair P4. Statistical analysis indicated that the PCR product size positively correlated with amylose content. The Wx gene locus was determined to be polymorphic, and was positively correlated with amylose. Based on the electrophoresis results, the 48 samples were divided into 4 types. PCR product types I, II, III, and IV were 457, 481, 489, and 491 bp in length, respectively, with the respective amylose content ranges of 4.9-27%, 29-30%, 31-35%, and 36-38%. Primer P4 can be used as a complementary marker for the selection of different amylose content hulless barley germplasms.
Keywords: Hordeum vulgare L. var. nudum; molecular marker; starch property; Waxy gene
Published: December 31, 2017 Show citation
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References
- Baik B.K., Steven E.U. (2008): Barley for food: characteristics, improvement, and renewed interest. Journal of Cereal Science, 48: 233-242.
Go to original source...
- Bao J.S., Corke H., Sun M. (2002): Microsatallites in starchsynthesizing genes in relation to starch physicochemical properties in waxy rice (Oryza sativa L.). Theoretical and Applied Genetics, 105: 898-905.
Go to original source...
Go to PubMed...
- Bollieh C.N., Webb B.D. (1973): Inheritance of amylose in two hybrid populations of rice. Cereal Chemistry, 50: 631-636.
- Briney A., Wilson R., Potter R.H., Barclay I., Crosbie G., Appels R., Jones M.G.K. (1998): A PCR-based marker for selection of starch and potential noodle quality in wheat. Molecular Breeding, 4: 427-433.
Go to original source...
- Chao S., Sharp P.J., Worland E.J. (1989): RFLP-based genetic maps of wheat homologous group 7 chromosomes. Theoretical and Applied Genetics, 78: 495-504.
Go to original source...
Go to PubMed...
- Clark J.R., Robertson M., Ainsworth C.C. (1991): Nucleotide sequence of a wheat (Triticum aestivum L.) cDNA clone encoding the waxy protein. Plant Molecular Biology, 16: 1099-1101.
Go to original source...
Go to PubMed...
- Denyer K., Barber L.M., Burton R., Hedley C.L., Hylton C.M., Johnson S. (1995): The isolation and characterization of novel low-amylose mutants of Pisum sativum L. Plant Cell and Environment, 18: 1019-1026.
Go to original source...
- Domon E., Saito A., Takeda K. (2002): Comparison of the waxy locus sequence from a non-waxy strain and two waxy mutants of spontaneous and artificial origins in barley. Genes and Genetic Systems, 77: 351-359.
Go to original source...
Go to PubMed...
- Domon E., Yanagisawa T., Saitol A., Takeda K. (2004): Single nucleotide polymorphism genotyping of the barley waxy gene by polymerase chain reaction with confronting twopair primers. Plant Breeding, 123: 225-228.
Go to original source...
- Hylton C.M., Denyer K., Keeling P.L., Chang M.T., Smith A.M. (1996): The effect of waxy mutation on the granulebound starch synthases of barley and maize endosperms. Planta, 198: 230-237.
Go to original source...
- Juliano B.O., Pascual C.G. (1980): Quality characteristics of milled rice grown in different countries. IRRI Research Paper Series, 48: 1-5.
- Kleinhofs A. (1997): Integrating barley RFLP and classical marker maps. Barley Genetics Newsletter, 27: 105-12.
- Kramer H.H., Blander B.A. (1961): Orientating linkage maps on the chromosomes of barley. Crop Science, 1: 339-342.
Go to original source...
- McKenzie K.S., Rutger J.N. (1983): Genetic analysis of amylose content alkali spreading score and grain dimensions in rice. Crop Science, 23: 306-313.
Go to original source...
- Nakamura T., Yamamori M., Hirano H., Hidaka S. (1993): Identification of three Wx proteins in wheat (Triticum aestivum L). Biochemical Genetics, 111: 75-86.
Go to original source...
Go to PubMed...
- Nakao S. (1950): On waxy barleys in Japan. Seiken Jiho, 4: 111-113.
Go to original source...
- Ono T., Suzuki H. (1957): Endosperm characters in hybrids between barley varieties with starchy and waxy endosperms. Seiken Jiho, 8: 11-19.
- Rosichan J., Nilan R.A., Arenaz P., Kleinhofs A. (1979): Intragenic recombination at the waxy locus in Hordeum vulgare. Barley Genetics Newsletter, 9: 79-85.
- Sharp P.J., Chao S., Desai S., Gale M.D. (1989): The isolation, characterization and application in Triticeae of a set of wheat RFLP probe identifying each homoeologous chromosome arm. Theoretical and Applied Genetics, 78: 342-348.
Go to original source...
Go to PubMed...
- Shure M.,Wessler S., Fedoroff N. (1983): Molecular identification and isolation of the Waxy locus in maize. Cell, 35: 225-233.
Go to original source...
Go to PubMed...
- Sun Y.Y., Lü Y., Dong C.L., Wang P.R., Huang X.Q., Deng X.J. (2005): Progress in regulation of rice Wx gene expression. Hereditas, 27: 79-85.
- Tabata M. (1961): Studies of a gametophyte factor in barley. The Japanese Journal of Genetics, 36: 157-167.
Go to original source...
- Vrinten P., Nakamura T., Yamamori M. (1999): Molecular characterization of waxy mutations in wheat. Molecular and General Genetics, 261: 463-471.
Go to original source...
Go to PubMed...
- Wang F., Zhao H., Wang Y., Wang X.Z. (2005): Relationship between fragment length polymorphism with waxy and amylase content in wheat. Journal of Plant Physiology and Molecular Biology, 31: 269-274.
- Wang H.P., Tang C.H., Liu S.X., Zhang L.P., Lu C.F. (2007): Analysis of waxy proteins in Shanxi winter wheat cultivars using SDS-PAGE and molecular markers. Acta Agriculture Boreali Sinica, 22: 98-102.
- Zhu C.M., Zhang J. (2010): Genetic diversity analysis of Waxy barley in China based on SSR markers. Journal of Plant Genetic Resources, 11: 564-572.
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