Czech Journal of Genetics and Plant Breeding, 2004 (vol. 40), issue 3
Importance of the Secondary Genepool in Barley Genetics and Breeding. I. Cytogenetics and Molecular Analysis
R. Pickering, P.A. Johnston, B. Ruge
Czech J. Genet. Plant Breed., 2004, 40(3):73-78 | DOI: 10.17221/3702-CJGPB 
There have been no plant breeding developments using species from the tertiary genepool of cultivated barley for breeding or genetics since the VIIIth International Barley Genetics Symposium in 2000. Hence, the first part of this review describes progress since 2000 in developing and characterising recombinant lines derived from hybridisations between the sole species in the secondary genepool, Hordeum bulbosum L., and cultivated barley, Hordeum vulgare L. The topics discussed in part I are cytogenetics and molecular analysis of recombinant lines.
Importance of the Secondary Genepool in Barley Genetics and Breeding II. Disease Resistance, Agronomic Performance and Quality
R. Pickering, R.E. Niks, P.A. Johnston, R.C. Butler
Czech J. Genet. Plant Breed., 2004, 40(3):79-85 | DOI: 10.17221/3703-CJGPB
In this second paper on the use of secondary genepools in barley improvement, we describe the characterisation of leaf rust resistant recombinant lines (RLs) derived from Hordeum vulgare × H. bulbosum crosses. Twelve RLs were inoculated with leaf rust and the early stages of disease development were observed. Several RLs showed complete resistance to the pathogen, but others had a high level of partial resistance, which may be durable. Some of these RLs and others were tested in yield trials to determine the effects of introgressed chromatin from H. bulbosum on yield and quality. We conclude that there are no major adverse...
Evaluation and Mapping of a Leaf Rust Resistance Gene Derived from Hordeum vulgare subsp. spontaneum
Doris Kopahnke, Marion Nachtigal, Frank Ordon, Brian J. Steffenson
Czech J. Genet. Plant Breed., 2004, 40(3):86-90 | DOI: 10.17221/3704-CJGPB
Studies of marker development were performed on a doubled haploid population derived from the cross of a highly resistant line H. spontaneum 677 × Krona (susceptible). Previous segregation studies on F2 and F3 populations revealed that the resistance of H. spontaneum 677 was likely due to a single dominant gene. Bulked segregant analysis using AFLPs and SSRs was conducted to identify markers linked to this leaf rust resistance gene. By this approach the resistance gene was located on barley chromosome 2H with the closest markers linked at 6.1 cM (E35M54b) and 13.6 cM (Bmac0218) based on the analysis...
Effect of Fusarium Head Blight on Mycotoxin Content in Grain of Spring Barley Cultivars
V. Šíp, L. Tvarůžek, J. Chrpová, S. Sýkorová, L. Leišová, L. Kučera, J. Ovesná
Czech J. Genet. Plant Breed., 2004, 40(3):91-101 | DOI: 10.17221/3705-CJGPB
The results are based on field infection experiments with six spring barley cultivars registered in the Czech Republic and resistance sources Chevron and CI 4196. One of the four Fusarium isolates used for inoculations was a predominant nivalenol producer, while the other isolates were deoxynivalenol (DON) producers. Out of the other mycotoxins 3-AcDON was found in grain at a relatively higher concentration. Significant cultivar differences in DON content, examined yield traits, percentage of Fusarium colonies and percentage of non-germinating seeds were detected after inoculum spraying on two dates and mist irrigation of infected...
A New Two Dimensional Germinative Classification of Malting Barley Quality Based on Separate Estimates of Vigour and Viability
B. MØLLER, L. Munck
Czech J. Genet. Plant Breed., 2004, 40(3):102-108 | DOI: 10.17221/3706-CJGPB
It is surprising that not even today do germination data seem fully integrated with malting data in barley quality evaluation. In order to implement such an integration, pattern recognition multivariate data analysis (chemometrics) is essential. Inspired by the results from chemometric analyses of whole germination curves we tested a two-dimensional classification plot of barley samples based on separate estimates for "vigour" (g%1) germination energy (GE) as abscissa with limits at 70% and 30% and "viability" (g%3) as ordinate with limits at 98% and 92%. The seven barley classes obtained visualise the quality differences in a consistent and instructive...
Spring wheat Granny
L. Bobková
Czech J. Genet. Plant Breed., 2004, 40(3):109-110 | DOI: 10.17221/6092-CJGPB