Czech J. Genet. Plant Breed., 2004, 40(3):91-101 | DOI: 10.17221/3705-CJGPB

Effect of Fusarium Head Blight on Mycotoxin Content in Grain of Spring Barley Cultivars

V. Šíp, L. Tvarůžek, J. Chrpová, S. Sýkorová, L. Leišová, L. Kučera, J. Ovesná
1 Research Institute of Crop Production, Prague-Ruzyně, Czech Republic
2 Agricultural Research Institute, Kroměříž, Czech Republic

The results are based on field infection experiments with six spring barley cultivars registered in the Czech Republic and resistance sources Chevron and CI 4196. One of the four Fusarium isolates used for inoculations was a predominant nivalenol producer, while the other isolates were deoxynivalenol (DON) producers. Out of the other mycotoxins 3-AcDON was found in grain at a relatively higher concentration. Significant cultivar differences in DON content, examined yield traits, percentage of Fusarium colonies and percentage of non-germinating seeds were detected after inoculum spraying on two dates and mist irrigation of infected plots. When inoculated with aggressive isolate of F. culmorum Chevron and CI 4196 cvs. showed high resistance and the cultivars Jersey, Olbram and Scarlett moderate resistance to DON accumulation in grain. Treatment with fungicide Horizon 250 EW (active ingredient tebuconazole) led on average to a 52.5% reduction of DON content, but the efficacy of fungicide treatment was highly influenced by year and cultivar. Fungicide treatment did not have a significant effect on grain weight per spike and, in general, the influence of infection on examined yield traits was low in these experiments. DON content was closely related only with the parameter CT Fus (transformed) from quantitative real time PCR analysis. Using the developed PCR system it was possible to specify clearly cultivar responses to infection and effects of fungicide treatment on DON content.

Keywords: spring barley; Fusarium culmorum; Fusarium graminearum; head blight; mycotoxin content; yield traits; cultivar resistance; fungicide treatment; quantitative PCR analysis

Published: September 30, 2004  Show citation

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Šíp V, Tvarůžek L, Chrpová J, Sýkorová S, Leišová L, Kučera L, Ovesná J. Effect of Fusarium Head Blight on Mycotoxin Content in Grain of Spring Barley Cultivars. Czech J. Genet. Plant Breed. 2004;40(3):91-101. doi: 10.17221/3705-CJGPB.
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    References

    1. A T., C J., G P., K Z., K M., P J., S M., W H. (1999): Yield reduction and mycotoxin accumulation in barley doubled haploids inoculated with Fusarium culmorum (W.G.Sm) Sacc. J. Appl. Genet., 40: 73-84.
    2. A D. (1996): Experience with the isolation of plant pathogenic fungi: In: D et al. (eds): Proc. 4th Int. Symp. EFPP Diagnosis and Identification of Plant Pathogens. 9-12 Sept. 1996, Bonn, Germany: 35-36. Go to original source...
    3. C H., C T.M., V B., U L. (2000): Mycotoxins in barley and oat samples from eastern Canada. Can. J. Plant Sci., 80: 977-980. Go to original source...
    4. C J., W H., A T., G P., K Z., K M., P J., S M. (2000): Effects of Fusarium culmorum head blight on mycotoxin accumulation and yield traits in barley doubled haploids. J. Phytopathol., 148: 541-545. Go to original source...
    5. D F.., P D.., J P., N P. (1998): The use of species-specific PCR-based assays to analyze Fusarium ear blight of wheat. Plant Pathol., 47: 197-205. Go to original source...
    6. D F.M., P D.W., N P. (1999): Fusarium ear blight of wheat: the use of quantitative PCR and visual disease assessment in studies of disease control. Plant Pathol., 48: 209-217. Go to original source...
    7. H C.A., S J., L K.J. W P.M. (1996): Real time quantitative PCR. Genome Res., 6: 986-994. Go to original source... Go to PubMed...
    8. J R.K. (2000): Assessments of Fusarium head blight of wheat and barley in response to fungicide treatment. Plant Dis., 84: 1021-1030. Go to original source... Go to PubMed...
    9. J R.K., M C.J. (1999): Quality parameters in small grains from Minnesota affected by Fusarium head blight. Plant Dis., 83: 506-511. Go to original source... Go to PubMed...
    10. M N., H R., C A., B E.S. (2002): Relationship between growth and mycotoxin production by Fusarium species, biocides and environment. Eur. J. Plant Pathol., 108: 685-690. Go to original source...
    11. M A., B T., L C. (2003): Influence of wheat cultivar, species of Fusarium, and isolate aggressiveness on the efficacy of fungicides for control of Fusarium head blight. Plant Dis., 87: 1107-1115. Go to original source... Go to PubMed...
    12. M H., W J. (1996): Investigations on the effect of various fungicides on the pathogen of partial head blight (Fusarium culmorum (W.G.Sm.) Sacc.). Nachr.-Bla Deutsch. Pfl.-Schutzd., 48: 93-95.
    13. M C.J., X W., X Y., W R.D., W R.P., E R.H., B G. (1994): Production of thrichothecene mycotoxins by Fusarium graminearum and Fusarium culmorum on barley and wheat. Mycopathologia, 128: 19-23. Go to original source... Go to PubMed...
    14. N P., C E., D R.C., G N.E., S D.R., T M., W A.H. (2003): Molecular tools to study epidemiology and toxicology of Fusarium head blight of cereals. Eur. J. Plant Pathol., 109: 691-703. Go to original source...
    15. P J., K I., S U., M M., C J., G P. (1997): Head infection and accumulation of Fusarium toxins in kernels of 12 barley genotypes inoculated with Fusarium graminearum isolates of two chemotypes. Eur. J. Plant Pathol., 103: 85-90.
    16. P E. (1999): Necrosis of barley grains, the germination and fungi isolated from grains. Rostl. Výr., 45: 133-144.
    17. R J.C., H R.D., M K A.L., E E.M. (2001): Host plant resistance genes for Fusarium head blight: Sources, mechanisms and utility in conventional breeding systems. Crop Sci., 41: 620-627. Go to original source...
    18. S B., S B.J., C H.H., T B., P L.K., F T.G. Jr., S P.B. (1999): Fusarium species pathogenic to barley and their associated mycotoxins. Plant Dis., 83: 667-674. Go to original source... Go to PubMed...
    19. Š V., S S., S E., C J. (2002a): The effect of infection with Fusarium culmorum L. on deoxynivalenol content in grain of selected winter wheat varieties. J. Appl. Genet., 43A: 319-332.
    20. Š V., S E., C J. (2002b): Evaluation of the response of selected winter wheat cultivars to artificial infection with Fusarium culmorum in field conditions. Petria, 12: 287-291.
    21. Š V., C J., S E. (2003): Analysis of factors that influenced accumulation of Fusarium mycotoxins in wheat grain. In: P N.E. et al. (eds): Proc. 10th Int. Wheat Genetics Symp., 1-6 Sept. 2003, Paestum, Italy: 1254-1256.
    22. Š V., C J., S S., L L., K L., O J. (2004): Assessment of cultivar differences in response to Fusarium head blight infection and fungicide treatment in winter wheat. In: V J., G H., R P. (eds): Proc. 17th EUCARPIA General Congr., 8-11 Sept. 2004, Tulln, Austria: 471.
    23. S B.J. (1998): Fusarium head blight of barley: epidemics, impact, and breeding for resistance. Techn. Quart., MBAA TQ, 35: 177-184.
    24. S B.J. (1999): Combating Fusarium head blight: an emerging threat to malting barley quality throughout the world. EBC Congr.: 531-538.
    25. S S., ŠÍP V., N M., S Z., H J., H J. (2003): The survey of Fusarium mycotoxins content in grain of winter wheat cultivars collected from different regions of Czech Republic. In: P N.E. et al. (eds): Proc. 10th Int. Wheat Genetics Symp., 1-6 Sept. 2003, Paestum, Italy: 1266-1268.
    26. T A., M C, B., G J. (2000): Fusarium head blight of barley in western Canada. Can. J. Plant Pathol., 22: 9-16. Go to original source...
    27. W C., K P., D V P.M., K Z., L, T.A.J., H T., K J., K G. (2003): Major changes in Fusarium spp. in wheat in the Netherlands. Eur. J. Plant Pathol., 109: 743-754. Go to original source...

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