Czech Journal of Genetics and Plant Breeding, 2021 (vol. 57), issue 3

TPR domain coding gene ST2 may be involved in regulating tillering and fertility in riceOriginal Paper

Gang Wei, Hongxia Yang, Zixian Xiong, Jingwen Wu, Danyang Chen, Yang Liu, Yijie Ban, Weichi Liu, Lina Shang, Nan Wang

Czech J. Genet. Plant Breed., 2021, 57(3):83-90 | DOI: 10.17221/103/2020-CJGPB  

A decrease in the tiller number and male sterility will lead to a decline in the rice yield. Therefore, it is significant to study the molecular mechanism of controlling the tiller number and regulating the male reproductive development. The mutant st2 (single tiller 2) was induced by ethyl methane sulfonate (EMS) in the indica maintainer line Xinong 1B and showed single tillering and male sterility. I₂-KI staining showed that the st2 pollen was aborted. The scanning electron microscope (SEM) observation underlined that the anther of st2 became smaller, the wax of the epidermis reduced, the inner wall...

Identification of known leaf rust resistance genes in bread wheat cultivars from ChinaOriginal Paper

Xiaocui Yan, Takele-Weldu Gebrewahid, Rui Dong, Xing Li, Peipei Zhang, Zhanjun Yao, Zaifeng Li

Czech J. Genet. Plant Breed., 2021, 57(3):91-101 | DOI: 10.17221/6/2021-CJGPB  

Leaf rust caused by Puccinia triticina Eriks. (Pt) is one of the most devastating fungal pathogens affecting wheat (Triticum aestivum L.) production worldwide. Deployment of resistant cultivars is the most environmentally friendly approach to control the disease. In this study, thirty-seven wheat lines from the Hubei and Shaanxi provinces in China were evaluated for seedling resistance in the greenhouse using eighteen Pt races. These lines were also tested for slow rusting resistance in the field in the 2014 to 2018 growing seasons. Eleven molecular markers closely associated with known Lr genes were used...

Construction of high-density genetic map and QTL mapping in Nicotiana tabacum backcrossing BC4F3 population using whole-genome sequencingOriginal Paper

Zhijun Tong, Sanjie Jiang, Weiming He, Xuejun Chen, Lixin Yin, Dunhuang Fang, Yafei Hu, Fangchan Jiao, Chi Zhang, Jianmin Zeng, Xinfu Wu, Shancen Zhao, Jianbo Jian, Bingguang Xiao

Czech J. Genet. Plant Breed., 2021, 57(3):102-112 | DOI: 10.17221/8/2021-CJGPB  

Backcrossing is a powerful tool for plant breeding. The improved marker-assisted backcrossing intends to transfer targeted genes or quantitative trait loci (QTLs) of interest from a donor parent into a recurrent parent. In this study, a tobacco BC4F3 population was generated using Y3 and K326 as hybrid parents and YF1-1 as F₁ parents. High-throughput sequencing data of 381 pedigree populations were used to construct high-density genetic maps containing 24 142 high-quality single nucleotide polymorphism (SNP) markers with an average genetic distance of 0.59 cM. A genome module analysis was then performed for all the offspring. A total of...

Mapping of quantitative trait loci for purple stigma and purple apiculus in rice by using a Zhenshan 97B/Minghui 63 RIL populationOriginal Paper

Jiping Tong, Zhengshu Han, Aonan Han

Czech J. Genet. Plant Breed., 2021, 57(3):113-118 | DOI: 10.17221/20/2021-CJGPB  

Anthocyanin pigmentation is an important morphological marker that is commonly used to identify rice varieties and for linkage analysis. The following study investigates the genetic factors involved in the purple stigma (Ps) and purple apiculus (Pa) traits of an important indica rice cross between Zhenshan 97 (purple stigma and purple apiculus) and Minghui 63 (grey stigma and colourless apiculus). A recombinant inbred line (RIL) population derived from this cross was used for quantitative trait loci (QTL) mapping of the purple stigma and purple apiculus traits. As a result, one major QTL for the purple stigma trait, temporarily designated...

Genome-wide identification and analysis of the MLO gene families in three Cucurbita speciesShort Communication

Lei Zhu, Yanman Li, Jintao Li, Yong Wang, Zhenli Zhang, Yanjiao Wang, Zanlin Wang, Jianbin Hu, Luming Yang, Shouru Sun

Czech J. Genet. Plant Breed., 2021, 57(3):119-123 | DOI: 10.17221/99/2020-CJGPB  

Powdery mildew (PM) is a major fungal disease in the Cucurbita species in the world, which can cause significant yield loss. The Mildew Locus O (MLO) family genes play important roles in the PM stress response. In this paper, twenty, twenty-one, and eighteen candidate MLO genes in Cucurbita moschata, Cucurbita maxima and Cucurbita pepo, respectively, were identified and designated as CmoMLO, CmaMLO and CpeMLO, respectively. The phylogenetic analysis indicated that these MLOs were divided into five clades and the number of MLOs belonging to clade V in the Cucurbita...