Czech Journal of Genetics and Plant Breeding, 2021 (vol. 57), issue 2

Effect of terminal heat stress on physiological traits, grain zinc and iron content in wheat (Triticum aestivum L.)Original Paper

Narendra M.C., Chandan Roy, Sudhir Kumar, Parminder Virk, Nitish De

Czech J. Genet. Plant Breed., 2021, 57(2):43-50 | DOI: 10.17221/63/2020-CJGPB  

Heat stress is one of the major wheat (Triticum aestivum) production constraints in South Asia (SA), particularly in the Eastern Gangetic Plains (EGP) of India and Bangladesh. Malnutrition is also a severe problem among children and women in SA. Wheat varieties with high grain Zn/Fe are a sustainable, cost-effective solution in the fight against hidden hunger. Thirty wheat genotypes were characterised under the optimum temperature and heat stress conditions in 2016-2017 and 2017-2018 to study the response of the stress on the yield, physiological traits and grain Zn/Fe content. A significant genetic variation was observed for all the traits...

Genetic analysis and molecular mapping of Rp, a mutant gene encoding red pericarp in rice (Oryza sativa L.)Original Paper

Jiping Tong, Zhengshu Han, Aonan Han

Czech J. Genet. Plant Breed., 2021, 57(2):51-57 | DOI: 10.17221/70/2020-CJGPB  

Coloured rice has pigments deposited in the grain pericarp; red rice is the most common type of coloured rice. Red rice is rich in essential nutrients and has been grown and consumed in China for a long time. In this study, we report the genetic characterisation and preliminary molecular mapping of a mutant gene encoding red pericarp in rice (Oryza sativa L.). To analyse the genetic basis of the red pericarp mutant, a reciprocal cross between GER-3 (red pericarp, indica cv.) and 898 (white pericarp, indica cv.) was made. The genetic analysis results confirmed that there was only one dominant gene, temporarily designated Rp (Red...

Phylogenetics of native conifer species in Vietnam based on two chloroplast gene regions rbcL and matKOriginal Paper

Mai Phuong Pham, Viet Ha Tran, Dinh Duy Vu, Quoc Khanh Nguyen, Syed Noor Muhammad Shah

Czech J. Genet. Plant Breed., 2021, 57(2):58-66 | DOI: 10.17221/88/2020-CJGPB  

We used two chloroplast gene regions (matK and rbcL) as a tool for the identification of 33 local conifer species. All 136 sequences, 101 newly generated (14 species for gene matK; 16 species for gene rbcL) and 35 retrieved from the GenBank, were used in the analysis. The highest genetic distance (matK region) was recorded between the species in Cupressaceae with an average of 5% (0.1-8.5), Podocarpaceae with an average of 6% (0-8.5), Taxaceae with an average of 5% (0.2-0.5) and Pinaceae with an average of 20.4% (0.8-54.1). The rbcL region showed a low genetic distance between the species in Cupressaceae...

Comparison of DNA methylation landscape between Czech and Armenian vineyards show their unique character and increased diversityOriginal Paper

Kateřina Baránková, Anna Nebish, Jan Tříska, Jana Raddová, Miroslav Baránek

Czech J. Genet. Plant Breed., 2021, 57(2):67-75 | DOI: 10.17221/90/2020-CJGPB  

Grapevine is a worldwide crop and it is also subject to global trade in wine, berries and grape vine plants. Various countries, including the countries of the European Union, emphasize the role of product origin designation and suitable methods are sought, able to capture distinct origins. One of the biological matrices that can theoretically be driven by individual vineyards' conditions represents DNA methylation. Despite this interesting hypothesis, there is a lack of respective information. The aim of this work is to examine whether DNA methylation can be used to relate a sample to a given vineyard and to access a relationship between a DNA methylation...

Allelic variations at the HvSNF2 and HvBM5 loci are associated with the heading date and growth habit of barley (Hordeum vulgare L.) under a semi-arid climateShort Communication

Salem Marzougui

Czech J. Genet. Plant Breed., 2021, 57(2):76-79 | DOI: 10.17221/62/2020-CJGPB  

The heading date and growth habit are key factors that regulate the transition from the vegetative to the reproductive stage in barley. In this study, we used PCR based markers to identify the allelic variations in the Vrn-H1 (HvMB5) and Vrn-H2 (HvSNF2) genes and to predict the heading date and growth habit of a collection of Tunisian barley assessed under a semi-arid climate. The allelic variation at HvBM5 revealed two PCR fragments at 830 and 344 bp. Primer sets used to amplify the HvSNF2 gene have resulted in different alleles size of 543, 623, and 700 bp. Different allelic combinations of HVBM5...

List of field crop varieties registered in the Czech Republic in 2020New Varieties

Tomáš Mezlík

Czech J. Genet. Plant Breed., 2021, 57(2):80-81 | DOI: 10.17221/13/2021-CJGPB