Czech J. Genet. Plant Breed., 2016, 52(3):120-123 | DOI: 10.17221/187/2015-CJGPB

Mapping and characterization of powdery mildew resistance gene in synthetic wheatShort Communication

Meha SHARMA, Satinder KAUR, Manny SALUJA, Parveen CHHUNEJA
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, India

Powdery mildew caused by Blumeria graminis f.sp. tritici is significantly affecting wheat production worldwide. In the search for new sources of resistance we investigated the powdery mildew resistance in the synthetic hexaploid wheat line "Synthetic 43" with its D genome from Aegilops tauschii. This line was developed at CIMMYT and resists a number of common bread wheat diseases. The line was crossed with the powdery mildew susceptible hexaploid wheat cultivar WH542 and a mapping population consisting of 148 RILs was developed. Inheritance studies in the RIL population revealed monogenic inheritance of powdery mildew resistance both at the seedling stage and adult plant stage. This resistance gene was mapped at a distance of 4.8 cM from SSR marker Xwmc150 on chromosome 7D and has been temporarily designated as PmT.

Keywords: Aegilops tauschii; Blumeria graminis; SSR; Triticum aestivum

Published: September 30, 2016  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
SHARMA M, KAUR S, SALUJA M, CHHUNEJA P. Mapping and characterization of powdery mildew resistance gene in synthetic wheat. Czech J. Genet. Plant Breed. 2016;52(3):120-123. doi: 10.17221/187/2015-CJGPB.
Download citation

References

  1. Blanco A., Gadaleta A., Cenci A., Carluccio A., Abdelbacki A.M.M., Simeone R. (2008): Molecular mapping of the novel powdery mildew resistance gene Pm36 introgressed from Triticum turgidum var. dicoccoides in durum wheat. Theoretical and Applied Genetics, 117: 135-142. Go to original source... Go to PubMed...
  2. Devos K.M., Chao S., Li Q.Y., Simonetti M.C., Gale M.D. (1994): Relationship between chromosome 9 of maize and wheat homoeologous group 7 chromosomes. Genetics, 138: 1287-1292. Go to original source... Go to PubMed...
  3. Hua W., Liu Z., Zhu J., Xie C., Yang T., Zhou Y., Duan X., Sun Q., Liu Z. (2009): Identification and genetic mapping of pm42, a new recessive wheat powdery mildew resistance gene derived from wild emmer (Triticum turgidum var. dicoccoides). Theoretical and Applied Genetics, 119: 223-230. Go to original source... Go to PubMed...
  4. Kaur S., Saini J., Sharma A., Singh K., Chhuneja P. (2012): Identification of variability in Blumeria graminis f.sp. tritici through molecular marker analysis. Crop Improvement, 39: 74-79.
  5. Kolmer J. (2013): Leaf rust of wheat: pathogen biology, variation and host resistance. Forests, 4: 70-84. Go to original source...
  6. Lorieux M. (2012): MapDisto: fast and efficient computation of genetic linkage maps. Molecular Breeding, 30: 1231-1235. Go to original source...
  7. Lubbers E.L., Gill K.S., Cox T.S., Gill B.S. (1991): Variation of molecular markers among geographically diverse accessions of Triticum tauschii. Genome, 34: 354-361. Go to original source...
  8. Lutz J., Hsam S.L.K., Limpert E., Zeller F.J. (1995): Chromosomal location of powdery mildew resistance genes in Triticum aestivum L. (common wheat). 2. Genes Pm2 and Pm19 from Aegilops squarrosa L. Heredity, 74: 152-156. Go to original source...
  9. McIntosh R.A., Yamazaki Y., Dubcovsky J., Rogers J., Morris C., Appels R., Xia X.C. (2013): Catalogue of gene symbols for wheat. In: Proc. 12 th Int. Wheat Genetics Symposium. Yokohama, Sept 8-13, 2013: 1-31.
  10. Michelmore R.W., Paran I., Kesseli R.V. (1991): Identification of markers linked to the disease resistance genes by bulked segregant analysis: rapid method to detect markers in specific genomic regions by using segregating population. Proceedings of National Academy of Sciences of the USA, 88: 9828-9832. Go to original source... Go to PubMed...
  11. Mujeeb-Kazi A., Gul A., Ahmad J., Mirza J.I. (2006): Simplified and effective protocol for production of bread wheat haploids (n = 3x = 21, ABD) with some application areas in wheat improvement. Pakistan Journal of Botany, 38: 393-406.
  12. Mujeeb-Kazi A., Gul A., Farooq M., Rizwan S., Ahmad I. (2008): Rebirth of synthetic hexaploids with global implications for wheat improvement. Australian Journal of Agricultural Research, 59: 391-398. Go to original source...
  13. Parks R., Carbone I., Murphy J.P., Marshall D., Cowger C. (2008): Virulence structure of the Eastern US wheat powdery mildew population. Plant Disease, 92: 1074-1082. Go to original source... Go to PubMed...
  14. Priestley R.H., Bayles R.A. (1998): The contribution and value of resistant cultivars to disease control in cereals. In: Clifford B.C., Leste R.E. (eds): Control of Plant Diseasecosts and Benefits. Oxford, Blackwell: 53-65.
  15. Saghai-Maroof M.A., Soliman K.M., Jorgensen R.A., Allard R.W. (1984): Ribosomal DNA pacer length polymorphism in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proceedings of National Academy of Sciences of the USA, 81: 8014-8018. Go to original source... Go to PubMed...
  16. Sharma M., Kaur S., Saini J., Bains N.S., Singh K., Chhuneja P. (2013): Genetics of stripe and leaf rust resistance in WH542 × Synthetic RIL population. Crop Improvement, 40: 11-16.
  17. Singh D.P., Sharma A.K., Singh D., Rana S.K., Singh K.P. (2009): Resistance to powdery mildew in Indian wheat. Plant Disease Research, 24: 942.
  18. Somers D.J., Isaac P., Edwards K. (2004): A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 109: 1105-1114. Go to original source... Go to PubMed...
  19. Todorovska E., Christov N., Slavov S., Christova P., Vassilev D. (2009): Biotic stress resistance in wheat-breeding and genomic selection implications. Biotechnology and Biotechnological Equipments, 23: 1410-1413. Go to original source...
  20. Tosa Y., Sakai K. (1990): The genetics of resistance of hexaploid wheat to the wheatgrass powdery mildew fungus. Genome, 33: 225-230. Go to original source...
  21. Zeller F.J., Kong L., Hart L., Mohler V., Hsam S.L.K. (2002): Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.) 7. Gene Pm29 in line Pova. Euphytica, 123: 187-194. Go to original source...

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.