Czech Journal of Genetics and Plant Breeding, 2016 (vol. 52), issue 3
Association of SNP markers with agronomic and quality traits of field pea in ItalyOriginal Paper
Barbara Ferrari, Massimo Romani, Gregoire Aubert, Karen Boucherot, Judith Burstin, Luciano Pecetti, Myriam Huart-Naudet, Anthony Klein, Paolo Annicchiarico
Czech J. Genet. Plant Breed., 2016, 52(3):83-93 | DOI: 10.17221/22/2016-CJGPB 
Only a few studies on pea (Pisum sativum) investigated the association of single nucleotide polymorphisms (SNP) markers with key agronomic traits. This study aimed to explore the association of a standard set of 384 SNP with grain yield, seed protein content, seed weight, onset of flowering, plant height and lodging susceptibility, in three connected bi-parental recombinant inbred line (RIL) populations including 90 lines each. These RIL originated from crosses between three cultivars that displayed high and stable grain yield across Italian environments, namely, Attika (A), Isard (I), and Kaspa (K). The 270 lines were phenotyped in a spring-sown...
Molecular characterization of snowdrop lectin (GNA) and its comparison with reported lectin sequences of AmaryllidaceaeOriginal Paper
Saber Delpasand Khabbazi, Allah Bakhsh, CengİZ Sancak, Sebahattin Özcan
Czech J. Genet. Plant Breed., 2016, 52(3):94-100 | DOI: 10.17221/58/2016-CJGPB
Plant lectins have become efficient sources of insect resistance in crops. The present study was conducted to identify, amplify, clone and characterize the plant lectin gene GNA. The lectin, present in Galanthus nivalis (snowdrop), is an agglutinin toxic to hemiptera. The attempt was made to elucidate the relationship of the lectin gene trGNA (GNA isolated and characterized from Turkey) with other previously cloned lectins having insecticidal activity and to ensure the presence of the conserved mannose-binding region/site in the gene sequence. The full-length cDNA of trGNA was 477 bp that contained a 333...
Identification and characterization of the yls mutation in rice (Oryza sativa L.) with lower photosynthetic pigment contentOriginal Paper
Sixue LIU, Limeng DENG, Yaping FU, Guocheng HU, Wenzhen LIU, Xin ZHAO
Czech J. Genet. Plant Breed., 2016, 52(3):101-107 | DOI: 10.17221/42/2016-CJGPB
Normal chloroplast development in rice is essential for photosynthesis and yield potential. To explore the physiological and molecular mechanism of chloroplast development, we isolated the rice mutant yls, which has yellow-green leaves at the rice seedling stage. In comparison with wild type (WT) plants, mutant plants had lower chlorophyll and carotenoid contents at the seedling stage. Transmission electron micrographs of the leaves of mutant plants showed abnormal grana stacking. We finally mapped the YLS gene within the BAC clone OSJNBa0032M21 of chromosome 11. Sequence analysis revealed the existence of a 33-bp deletion within...
Reaction of wheat to common bunt and dwarf bunt and reaction of triticale to dwarf buntOriginal Paper
Veronika DUMALASOVÁ, Pavel BARTO©
Czech J. Genet. Plant Breed., 2016, 52(3):108-113 | DOI: 10.17221/48/2016-CJGPB
Resistance to common bunt and to dwarf bunt in winter wheat cultivars recently registered in the Czech Republic was studied in artificially inoculated field trials in the years 2013-2015. In trials with common bunt, seeds of each experimental series were inoculated with a different mixture of isolates. In dwarf bunt trials, the soil surface was inoculated with a natural bunt population from a single locality. Several selected unregistered wheat cultivars, triticale cultivars and cultivars/lines known as sources of dwarf bunt resistance were also included in the trials with dwarf bunt. Out of the recently registered winter wheat cultivars only cv. Genius...
Callus induction and frond regeneration in Spirodela polyrhizaOriginal Paper
Youru WANG
Czech J. Genet. Plant Breed., 2016, 52(3):114-119 | DOI: 10.17221/134/2015-CJGPB
Spirodela polyrhiza belongs to the family Lemnaceae (duckweed), which is a group of small aquatic plants offering an attractive plant expression system for the production of recombinant protein. No frond regeneration protocol has been established in this species yet. An efficient protocol for plant regeneration through organogenesis has been developed in Spirodela polyrhiza for the first time. Calli were successfully induced from 92% of explants on Murashige and Skoog (MS) medium with 10 μM naphthaleneacetic acid, 2 μM thidiazuron, 1μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 3% sucrose. MS medium containing 1% (m/v) sorbitol...
Mapping and characterization of powdery mildew resistance gene in synthetic wheatShort Communication
Meha SHARMA, Satinder KAUR, Manny SALUJA, Parveen CHHUNEJA
Czech J. Genet. Plant Breed., 2016, 52(3):120-123 | DOI: 10.17221/187/2015-CJGPB
Powdery mildew caused by Blumeria graminis f.sp. tritici is significantly affecting wheat production worldwide. In the search for new sources of resistance we investigated the powdery mildew resistance in the synthetic hexaploid wheat line "Synthetic 43" with its D genome from Aegilops tauschii. This line was developed at CIMMYT and resists a number of common bread wheat diseases. The line was crossed with the powdery mildew susceptible hexaploid wheat cultivar WH542 and a mapping population consisting of 148 RILs was developed. Inheritance studies in the RIL population revealed monogenic inheritance of powdery mildew resistance...