Czech J. Genet. Plant Breed., 2010, 46(10):S54-S56 | DOI: 10.17221/2447-CJGPB

Genetic diversity in portuguese native Vitis vinifera L. ssp. vinifera and ssp. sylvestris

J. Cunha1,2, M. Teixeira Santos3, J. Brazão1, L.C. Carneiro3, M. Veloso3, P. Fevereiro2,4, J.E.J. Eiras-Dias1
1 INRB, L-INIA, Quinta d'Almoinha, 2565-191 Dois Portos, Portugal
2 Universidade Nova de Lisboa, ITQB, 2784-505 Oeiras, Portugal
3 INRB, L-INIA, 2784-505 Oeiras, Portugal
4 Universidade de Lisboa, Faculta Ciências, Departement Biologia Vegetal, Campo Grande, 1749-016 Lisboa, Portugal, e-mail: evn.eiras.dias@mail.net4b.pt

To assess the different origins of Portuguese grapevine varieties, we used six nuclear and four chloroplastidal microsatellites as molecular markers, in order to compare the genetic structure of native wild-vines with native grapevine varieties. Both native subspecies have a great diversity, and a high interrelationship across the six nuclear microsatellites. Although identical numbers of alleles were found in each population, their distribution was different in the vinifera and sylvestris subspecies. Portuguese wild-vines have only chlorotypes A and B; A being the most frequent. The fifty-seven analysed Portuguese varieties have chlorotypes A, B, C, and D. The most frequent was the chlorotype A (75%), followed by D (21%). The results obtained reinforced the idea of Western Europe as having been one of the domestication centres for the grapevine, with contributions from the Eastern European gene pool. The observed genetic structure is a starting point from which to clarify the high number of native cultivars found in Portugal, and reinforces their probable origin in the Iberian Peninsula.

Keywords: domestication centre; genetic structure; Portuguese vinifera and sylvestris subspecies; vine

Published: December 31, 2010  Show citation

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Cunha J, Teixeira Santos M, Brazão J, Carneiro LC, Veloso M, Fevereiro P, Eiras-Dias JEJ. Genetic diversity in portuguese native Vitis vinifera L. ssp. vinifera and ssp. sylvestris. Czech J. Genet. Plant Breed. 2010;46(Special Issue):S54-56. doi: 10.17221/2447-CJGPB.
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References

  1. Almadanim M.C., Baleiras-Couto M.M., Pereira H.S., Carneiro L.C., Fevereiro P., Eiras-Dias J.E., Morais L., Viegas W., Veloso M.M. (2007): Genetic diversity of the grapevine (Vitis vinifera L.) varieties most utilized for wine production in Portugal. Vitis, 46: 116-119.
  2. Arroyo-Garcia R., Ruiz-Garcia L., Bolling L., Ocete R., Lopez M.A. et al. (2006): Multiple origins of cultivated grapevine (Vitis vinifera L. ssp. sativa) based on chloroplast DNA polymorphisms. Molecular Ecology, 15: 3707-3714. Go to original source... Go to PubMed...
  3. Cunha J., Teixeira Santos M., Carneiro L.C., Fevereiro P., Eiras-Dias J.E.J. (2009): Traditional grapevine cultivars and wild vines (Vitis vinifera L.) are related in Portugal. Genetic Resources and Crop Evolution, 56: 975-989. DOI 10.1007/s10722-0099416-4. Go to original source...
  4. Grassi F., Labra M., Imazio S., Spada A., Sgorbati S., Scienza A., Sala F. (2003): Evidence of a secondary grapevine domestication centre detected by SSR analysis. Theoretical and Applied Genetics, 107: 1315-1320. Go to original source... Go to PubMed...
  5. Imazio S., Labra M., Grassi F., Scienza A., Failla O. (2006): Chloroplast microsatellites to investigate the origin of grapevine. Genetic Resources and Crop Evolution, 53: 1003-1011. Go to original source...
  6. Peakall R., Smouse P.E. (2006): GENALEX 6: genetic analysis in Excel, population genetic software for teaching and research. Molecular Ecology Notes, 6: 288-295. DOI 10.1111/j.1471-8286.2005.01155.x Go to original source...
  7. Thomas M.R., Matsumoto S., Cain P., Scott N.S. (1993): Repetitive DNA of grapevine: classes present and sequences suitable for cultivar identification. Theoretical and Applied Genetics, 86: 173-180. Go to original source... Go to PubMed...

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