Czech J. Genet. Plant Breed., 2010, 46(1):35-40 | DOI: 10.17221/52/2009-CJGPB

Freezing tolerance and proline content of in vitro selected hydroxyproline resistant winter oilseed rapeShort Communication

Anna JANSKÁ1, 2, Sylva ZELENKOVÁ1, Miroslav KLÍMA2, Miroslava VYVADILOVÁ2, Ilja T. PRÁŠIL2
1 Department of Plant Physiology, Faculty of Science, Charles University in Prague, Czech Republic
2 Crop Research Institute, Prague-Ruzyně, Czech Republic

Twelve doubled haploid (DH) winter oilseed rape plants with altered levels of proline and/or freezing tolerance were obtained by in vitro selection for resistance to trans-4-hydroxy-l-proline (Hyp) in five segregating microspore populations. No significant response to selection either in proline content or in freezing tolerance, compared with the non-selected control populations, was observed. When data from all examined materials were combined, a weak correlation between proline content and freezing tolerance was observed.

Keywords: abiotic stress; Brassica napus; microspore culture; proline

Published: March 31, 2010  Show citation

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JANSKÁ A, ZELENKOVÁ S, KLÍMA M, VYVADILOVÁ M, PRÁŠIL IT. Freezing tolerance and proline content of in vitro selected hydroxyproline resistant winter oilseed rape. Czech J. Genet. Plant Breed. 2010;46(1):35-40. doi: 10.17221/52/2009-CJGPB.
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