Czech J. Genet. Plant Breed., 2009, 45(3):117-122 | DOI: 10.17221/35/2009-CJGPB

Broadening the genetic base of crop brassicas by production of new intergeneric hybridOriginal Paper

Aparajita MOHANTY1, Babeeta CHRUNGU2, Nidhi VERMA3, Kundaranahalli Ramalingaiah SHIVANNA4
1 Department of Botany, Gargi College, University of Delhi, New Delhi, India
2 Zakir Husain College, Jawaharlal Nehru Marg, New Delhi, India
3 National Bureau of Plant Genetic Resources, IARI Campus, New Delhi, India
4 Ashoka Trust for Research in Ecology and the Environment, Hebbal, Bangalore, India

Wide hybridization between crop brassicas and their wild relatives is an important approach towards increasing the genetic variability, which can be utilised for brassica breeding programs. A new intergeneric hybrid between Erucastrum cardaminoides and Brassica oleracea var. alboglabra was produced using embryo rescue techniques. The F1 hybrid was intermediate between the male and female parent for most of the morphological characters. Cytological studies of pollen mother cells of the hybrid revealed a preponderance of univalents at metaphase I. The number of bivalents in the digenomic hybrid was lower than expected. However, the presence of trivalent and quadrivalent in cells indicated some homoeology between the two genomes and hence the possibility of introgression of genes into the cultivar. The first backcross progeny was obtained using B. oleracea var. alboglabra as the pollen parent. Further, it is being used for developing new alloplasmic lines. The intergeneric hybrid was also used as bridge species to transfer wild (E. cardaminoides) cytoplasm to B. napus and B. carinata. The new intergeneric hybrid and bridge cross hybrids produced in the present investigation have contributed towards increasing the genic and cytoplasmic variability and thus broadening the genetic base of crop brassicas.

Keywords: Brassica oleracea var. alboglabra; embryo-rescue; Erucastrum cardaminoides genetic variability; wide hybridization

Published: September 30, 2009  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
MOHANTY A, CHRUNGU B, VERMA N, SHIVANNA KR. Broadening the genetic base of crop brassicas by production of new intergeneric hybrid. Czech J. Genet. Plant Breed. 2009;45(3):117-122. doi: 10.17221/35/2009-CJGPB.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Armstrong K.C., Keller W.A. (1982): Chromosome pairing in haploids of Brassica oleracea. Canadian Journal of Genetics and Cytology, 24: 735-739. Go to original source...
    2. Bang S.W., Sugihara K., Jeung B.H., Kaneko R., Satake E., Kaneko Y., Matsuzawa Y. (2007): Production and characterization of intergeneric hybrids between Brassica oleracea and a wild relative Moricandia arvensis. Plant Breeding, 126: 101-103. Go to original source...
    3. Bhat S.R., Kumar P., Prakash S. (2007): An improved cytoplasmic male sterile (Diplotaxis berthautii) Brassica juncea: identification of restorer and molecular characterization. Euphytica, 159: 145-152. Go to original source...
    4. Chandra A., Gupta M.L., Ahuja I., Kaur G., Banga S.S. (2004): Intergeneric hybridization between Erucastrum cardaminoides and two diploid crop Brassica species. Theoretical and Applied Genetics, 108: 1620-1626. Go to original source... Go to PubMed...
    5. Garg H., Banga S., Bansal P., Atri C., Banga S.S. (2007): Hybridizing Brassica rapa with wild crucifers Diplotaxis erucoides and Brassica maurorum. Euphytica, 156: 417-424. Go to original source...
    6. Gomez-Campo C. (1984): Studies on Cruciferae: XI Erucastrum ifniense Gomez-Campo, sp. nov., and its allies. Anales Jardin Botanico de Madrid, 41: 83-85.
    7. Gupta S.K., Sharma T.R., Chib H.S. (1995): Evaluation of wild allies of Brassica under natural conditions. Cruciferae Newsletter, 17: 10-11.
    8. Jenczewski E., Eber F., Grimaud A., Huet S., Lucas M.O., Monod H., Chevre A.M. (2003): Prbn, a major gene controlling homeologous pairing in oilseed rape (Brassica napus) haploids. Genetics, 164: 645-653. Go to original source... Go to PubMed...
    9. Lysak M.A., Koch M.A., Pecinka A., Schubert I. (2005): Chromosome triplication found across the tribe Brassiceae. Genome Research, 15: 516-525. Go to original source... Go to PubMed...
    10. Murashige T., Skoog F. (1962): A revised media for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15: 473-497. Go to original source...
    11. Nicolas S.D., Leflon M., Liu Z., Eber F., Chelysheva L., Coriton O., Chevre A.M., Jenczewski E. (2008): Chromosome 'speed dating' during meiosis of polyploid Brassica hybrids and haploids. Cytogenetic and Genome Research, 120: 331-338. Go to original source... Go to PubMed...
    12. Prakash S. (2001): Utilization of wild germplasm of Brassica allies in developing cytoplasmic male sterility-fertility restoration systems in Indian mustard Brassica juncea. In: Liu H., Fu T.D. (eds): Proc. Int. Symp. Rapeseed Science. Science Press, New York, 63-67.
    13. Primard-Brisset C., Poupard J.P., Horvais R., Eber F., Pelletier G., Renard M., Delourme R. (2005): A new recombined double low restorer line for the OguINRA cms in rapeseed (Brassica napus L.). Theoretical and Applied Genetics, 111: 736-746. Go to original source... Go to PubMed...
    14. Quiros C.F. (1999): Genome structure and mapping. In: Gomez-Campo C (ed.). Biology of Brassica coenospecies. Elsevier Science, Amsterdam. Go to original source...
    15. Rao G.U., Pradhan A.K., Shivanna K.R. (1998): Isolation of useful variants in alloplasmic crop brassicas in the cytoplasmic background of Erucastrum gallicum. Euphytica, 103: 301-306. Go to original source...
    16. Shivanna K.R. (1996): Incompatibility and wide hybridization. In: Chopra V.L., Prakash S. (eds): Oilseed and Vegetable Brassicas: Indian Perspective. Oxford & IBH Publishers, New Delhi, 77-102.
    17. Shivanna K.R., Rangaswamy N.S. (1992): Pollen Biology: A Laboratory Manual. Springer-Verlag, Heidelberg. Go to original source...
    18. Sigareva M.A., Earle E.D. (1997): Direct transfer of a cold-tolerant Ogura male-sterile cytoplasm into cabbage (Brassica oleracea ssp. capitata) via protoplast fusion. Theoretical and Applied Genetics, 94: 213-220. Go to original source...
    19. Warwick S.I., Francis A., L.A. Fleche J. (2000): Guide to Wild Germplasm of Brassica and Allied Crops (tribe Brassiceae, Brassicaceae). Agriculture and Agri-Food Canada, Ottawa.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content