Czech Journal of Genetics and Plant Breeding, 2009 (vol. 45), issue 3
Cereal resistance to Fusarium head blight and possibilities of its improvement through breedingReview
Klára Kosová, Jana Chrpová, Václav Šíp
Czech J. Genet. Plant Breed., 2009, 45(3):87-105 | DOI: 10.17221/63/2009-CJGPB 
The aim of this review is to summarize recent information on Fusarium head blight (FHB) in small grain cereals, especially in wheat and barley. Basic information on FHB epidemiology, types of resistance and plant resistance mechanisms is included. Standard methods for the evaluation of the individual types of FHB resistance and the extent of infection are briefly described. Special attention is paid to the sources of FHB resistance of different origin and possibility of their exploitation in cereal breeding. Unfortunately, a high level of FHB resistance was detected in non-adapted germplasm or distant relatives, which is a serious impediment to breeding...
EST-SNPs in bread wheat: discovery, validation, genotyping and haplotype structureOriginal Paper
Sachin Rustgi, Rajib Bandopadhyay, Harindra S. Balyan, Pushpendra K. Gupta
Czech J. Genet. Plant Breed., 2009, 45(3):106-116 | DOI: 10.17221/16/2009-CJGPB
The present study involves discovery, validation and use of single-nucleotide polymorphisms (SNPs) in bread wheat utilizing 48 EST-contigs (individual contigs having 20-89 ESTs, derived from 2 to 11 different genotypes). In order to avoid a problem due to homoeologous relationships, the ESTs in each contig were classified into 175 sub-contigs (3.7 sub-contigs/EST-contig) using characteristic homoeologue sequence variants (HSVs), which had a density of 1 HSV every 136.7 bp. In silico analysis of sub-contigs led to the discovery of 230 candidate EST-SNPs with a density of 1SNP/273.9 bp. Locus specific primers (each primer pair flanking...
Broadening the genetic base of crop brassicas by production of new intergeneric hybridOriginal Paper
Aparajita MOHANTY, Babeeta CHRUNGU, Nidhi VERMA, Kundaranahalli Ramalingaiah SHIVANNA
Czech J. Genet. Plant Breed., 2009, 45(3):117-122 | DOI: 10.17221/35/2009-CJGPB
Wide hybridization between crop brassicas and their wild relatives is an important approach towards increasing the genetic variability, which can be utilised for brassica breeding programs. A new intergeneric hybrid between Erucastrum cardaminoides and Brassica oleracea var. alboglabra was produced using embryo rescue techniques. The F1 hybrid was intermediate between the male and female parent for most of the morphological characters. Cytological studies of pollen mother cells of the hybrid revealed a preponderance of univalents at metaphase I. The number of bivalents in the digenomic hybrid was lower than expected. However, the...
Development of fertility restorers of winter oilseed rape with low glucosinolate content for the CMS Ogu-INRA systemOriginal Paper
Radoslav KOPRNA, Vratislav KUČERA, Ivana MACHÁČKOVÁ, Jiří HORÁČEK, Jaroslava EHRENBERGEROVÁ
Czech J. Genet. Plant Breed., 2009, 45(3):123-127 | DOI: 10.17221/71/2008-CJGPB
We have bred low glucosinolate (GSL) winter oilseed rape lines carrying the fertility restorer for the CMS Ogu-INRA system. The original restorer line BO20 contained 31μmol/g GSL in seeds, but by crossing this line with various low GSL CMS lines, followed by repeated selection of fertile segregants, we were able to obtain fertile lines with a mean GSL content in seeds of 11.8 μmol/g. This result confirmed that the gene(s) controlling the GSL content are not closely linked to the fertility restorer gene. The results confirm, that the SCAR marker SG34 is closely associated with the fertility restoring allele, and facilitates so the selection of...
10th International symposium on the biosafety of genetically modified organisms (GMOs)Conference Report
S. Rakouský
Czech J. Genet. Plant Breed., 2009, 45(3):128-130 | DOI: 10.17221/45/2009-CJGPB
Small timothy ZubrNew Varieties
B. Cagaš
Czech J. Genet. Plant Breed., 2009, 45(3):131-132 | DOI: 10.17221/47/2009-CJGPB
Perennial ryegrass ZekolNew Varieties
B. Cagaš
Czech J. Genet. Plant Breed., 2009, 45(3):133-134 | DOI: 10.17221/49/2009-CJGPB