Czech J. Genet. Plant Breed., 2022, 58(1):1-9 | DOI: 10.17221/34/2021-CJGPB

Isolation, sequencing of the HvnHID gene and its role in the purple-grain colour development in Tibetan hulless barleyOriginal Paper

Xiaohua Yao1,2,3,4, Leping Su1,5, Youhua Yao1,2,3,4, Likun An1,2,3,4, Yixiong Bai1,2,3,4, Xin Li1,2,3,4, Kunlun Wu*,1,2,3,4
1 Qinghai University, Xining, P.R. China
2 Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China
3 Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, P.R. China
4 Qinghai Subcenter of National Hulless Barley Improvement, Xining, P.R. China
5 Yan'an Institute of Agricultural Sciences, Yan'an, P.R. China

2-hydroxyisoflavanone dehydratase (HID) plays an important role in isoflavone biosynthesis. In this study, HID was isolated from the seeds of the purple-grained Tibetan hulless barley variety Nerumuzha and the white-grained variety Kunlun 10. The HvnHID gene includes the 981 bp open reading frame and encodes a protein of 327 amino acids. It has a typical Abhydrolase_3 domain (78-306) and belongs to the carboxylesterase (CXE) family of the Abhydrolase_3 (α/β hydrolase) superfamily. There are eight nucleotide differences in the HvnHID coding sequence and two amino acid differences (one in the Abhydrolase_3 domain) between Nerumuzha and Kunlun 10. The HvnHID of hulless barley has the closest relationship with the HID in Hordeum vulgare, and the most distant relationship in Panicum hallii. At the early-mid stage of the seed colour development, the HvnHID expression levels in the purple and black seeds were significantly higher than in the white and blue ones (P < 0.01). During the seed colour development of purple-grained hulless barley, the expression of the key genes (HvnF3'H, HvnDRF, HvnANT1, and HvnGT) in the anthocyanidin biosynthetic pathway increased significantly, while the HvnHID expression decreased significantly (P < 0.01). Thus, it is likely that HvnHID negatively regulates the anthocyanidin biosynthesis. This result provides an important basis for further study of the biological functions of HvnHID in the anthocyanidin biosynthetic pathway.

Keywords: 2-hydroxyisoflavanone dehydratase; anthocyanidin; isoflavones; purple grain

Published: December 17, 2021  Show citation

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Yao X, Su L, Yao Y, An L, Bai Y, Li X, Wu K. Isolation, sequencing of the HvnHID gene and its role in the purple-grain colour development in Tibetan hulless barley. Czech J. Genet. Plant Breed. 2022;58(1):1-9. doi: 10.17221/34/2021-CJGPB.
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