Czech J. Genet. Plant Breed., 2022, 58(1):29-35 | DOI: 10.17221/55/2021-CJGPB

Disease resistance of improved MR220 lines against Pyricularia oryzae Cavara and their preliminary agronomic performanceOriginal Paper

Siti Nor Aziemah Mohamad1, Mohamad Bahagia Ab Ghaffar ORCID...*,2, Ahmad Sofiman Othman1, Siti Norsuha Misman2, Zuraida Abd Rahman3, Mohd Shahril Firdaus Ab Razak3, Zulkifli Ahmad Seman3, Muhammad Fairuz Mohd Yusof3, Khairulmazmi Ahmad4, Siti Norhidaya Yazid4, Habibuddin Hashim5
1 School of Biological Science, Universiti Sains Malaysia, Penang, Malaysia
2 Research Innovation Centre Excellence, Malaysian Agricultural Research and Development Institute (MARDI), Seberang Perai, Kepala Batas, Penang, Malaysia
3 Biotechnology & Nanotechnology Research Centre, MARDI Headquarters, Serdang, Selangor, Malaysia
4 Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
5 Agric Inov Resources, Sg Petani, Kedah, Malaysia

Blast disease caused by Pyricularia oryzae is one of the most destructive fungal diseases of rice in Malaysia. Utilisation of resistant varieties is the most efficient management approach towards reducing yield losses. The line IRTP21683 with the Pi9 gene has shown strong resistance against the isolate MPO988.3 of pathotype P0.0, the most prevalent P. oryzae pathotype in Malaysia. Crossing of IRTP21683 was undertaken with the recurrent parent MR220, a susceptible elite Malaysian rice variety, using a marker assisted backcrossing technique with two simple sequence repeat markers, RM19776 and RM7311, as the tag for the Pi9 gene. Twenty BC3F4 lines with the Pi9 gene were resistant when challenged with MPO 988.3. The cluster analysis based on seven agronomic parameters showed that the resistant BC3F4 lines could be divided into four groups, of which the members in group 1 and 2 have shown comparable or better performance than MR220. Five lines in group 1, B220PI9-3-48, B220PI9-3-76, B220PI9-3-77, B220PI9-3-79 and B220PI9-3-82 showed outstanding yield performance with early maturation.

Keywords: blast disease; marker assisted backcrossing (MAB); Pyricularia oryzae; Pi9 gene; rice

Published: December 17, 2021  Show citation

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Mohamad SNA, Ab Ghaffar MB, Othman AS, Misman SN, Rahman ZA, Ab Razak MSF, et al.. Disease resistance of improved MR220 lines against Pyricularia oryzae Cavara and their preliminary agronomic performance. Czech J. Genet. Plant Breed. 2022;58(1):29-35. doi: 10.17221/55/2021-CJGPB.
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