Czech J. Genet. Plant Breed., 2021, 57(3):102-112 | DOI: 10.17221/8/2021-CJGPB

Construction of high-density genetic map and QTL mapping in Nicotiana tabacum backcrossing BC4F3 population using whole-genome sequencingOriginal Paper

Zhijun Tong1, Sanjie Jiang2, Weiming He2, Xuejun Chen1, Lixin Yin2, Dunhuang Fang1, Yafei Hu2, Fangchan Jiao1, Chi Zhang2, Jianmin Zeng1, Xinfu Wu1, Shancen Zhao3, Jianbo Jian*,2,4, Bingguang Xiao*,1
1 Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China
2 BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China
3 BGI Institute of Applied Agriculture, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China
4 Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, Denmark

Backcrossing is a powerful tool for plant breeding. The improved marker-assisted backcrossing intends to transfer targeted genes or quantitative trait loci (QTLs) of interest from a donor parent into a recurrent parent. In this study, a tobacco BC4F3 population was generated using Y3 and K326 as hybrid parents and YF1-1 as F1 parents. High-throughput sequencing data of 381 pedigree populations were used to construct high-density genetic maps containing 24 142 high-quality single nucleotide polymorphism (SNP) markers with an average genetic distance of 0.59 cM. A genome module analysis was then performed for all the offspring. A total of forty-three candidate QTLs for six agronomics traits were identified. This study provides original biomarkers for tobacco breeding and offers clues for prospective backcrossing applications in other plants.

Keywords: crossing; single nucleotide polymorphism (SNP); whole genome sequencing (WGS)

Published: July 14, 2021  Show citation

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Tong Z, Jiang S, He W, Chen X, Yin L, Fang D, et al.. Construction of high-density genetic map and QTL mapping in Nicotiana tabacum backcrossing BC4F3 population using whole-genome sequencing. Czech J. Genet. Plant Breed. 2021;57(3):102-112. doi: 10.17221/8/2021-CJGPB.
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