Czech Journal of Genetics and Plant Breeding, 2017 (vol. 53), issue 1

LIST OF REVIEWERS 2016

editors

Czech J. Genet. Plant Breed., 2017, 53(1):I | DOI: 10.17221/9572-CJGPB  

Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selectionOriginal Paper

Teerawat SUWANNUAL, Sompong CHANKAEW, Tidarat MONKHAM, Weerasak SAKSIRIRAT, Jirawat SANITCHON

Czech J. Genet. Plant Breed., 2017, 53(1):1-8 | DOI: 10.17221/51/2016-CJGPB  

Thai rice cultivar RD6 is well known for its cooking and eating qualities. However it is susceptible to blast disease, a major rice disease caused by the fungus Magnaporthe oryzae. This study focused on the pyramiding of four QTLs for blast resistance located on chromosomes 1, 2, 11 and 12, from two RD6 introgression lines. Marker-assisted selection was performed and facilitated the selection with 8 microsatellite flanking markers to enable the selection in BC2F2:3 lines. All possible combinations of the four QTL alleles were assessed for blast resistance by artificial inoculation using 8 diverse isolates in a greenhouse...

Relative efficiency of positive selection and tissue culture for generating pathogen-free planting materials of yam (Dioscorea spp.)Original Paper

Morufat BALOGUN, Norbert MAROYA, Joao AUGUSTO, Adeola AJAYI, Lava KUMAR, Beatrice AIGHEWI, Robert ASIEDU

Czech J. Genet. Plant Breed., 2017, 53(1):9-16 | DOI: 10.17221/117/2016-CJGPB  

Yams are staples in West Africa. They are propagated from tubers in an informal seed system. This encourages a build-up of diseases, and necessitates the rapid development of a formal seed system where certified seeds are functional. Although few reports exist on the use of meristem culture to generate pathogen-free yam, the success rate for the most economically important species in the sub-region, Dioscorea rotundata, for the most prevalent viruses is inadequate. To generate pathogen-free yam planting materials, the relative efficiency of tissue culture and positive selection was compared. Twenty-one asymptomatic yam plants were positively...

The effect of copper on plant regeneration in barley microspore cultureOriginal Paper

Katarzyna MAKOWSKA, Sylwia OLESZCZUK, Janusz ZIMNY

Czech J. Genet. Plant Breed., 2017, 53(1):17-22 | DOI: 10.17221/82/2016-CJGPB  

Isolated microspore culture is an excellent system for the production of doubled haploids in many crops, including barley. In a more traditional barley anther culture method copper sulphate is known to enhance plant regeneration. Here we report that one hundred times higher concentration of copper sulphate in the isolated microspore culture of two spring barley genotypes compared to the standard content in the induction medium resulted in a 34% increase of total plant regeneration. Detailed analysis of plant regeneration showed that additional supplementation of copper sulphate increased not only the regeneration of green plants but also proportionately...

Development of a muskmelon cultivar with improved resistance to gummy stem blight and desired agronomic traits using gene pyramidingOriginal Paper

Ning ZHANG, Bing-hua XU, Yan-fei BI, Qun-feng LOU, Jin-feng CHEN, Chun-tao QIAN, Yong-bing ZHANG, Hong-ping YI

Czech J. Genet. Plant Breed., 2017, 53(1):23-29 | DOI: 10.17221/84/2016-CJGPB  

Gummy stem blight (GSB), caused by Didymella bryoniae (Auersw.) Rehm., is a severe disease affecting Cucurbitaceae crops including melons. The resistance of current melon varieties that carry a single Gsb resistance gene is insufficient to protect against the abundant variation of the D. bryoniae isolates. Pyramiding multiple Gsb resistance genes into melon cultivars is an effective way to develop a broad resistance spectrum and to increase the duration of GSB resistance. In this study, two resistance genes (Gsb-4 and Gsb-6) from two resistant accessions, PI482398 and PI420145, were pyramided...

Expression profiling of certain MADS-box genes in Arabidopsis thaliana plant treated with silver nanoparticlesOriginal Paper

Zainab Majed ALMUTAIRI

Czech J. Genet. Plant Breed., 2017, 53(1):30-36 | DOI: 10.17221/130/2015-CJGPB  

Silver nanoparticles (AgNPs) have been shown to enhance seed germination and plant growth. In this study, we examined the plant response to AgNP dosage using a model plant, Arabidopsis thaliana. Seedlings were treated with different concentrations of AgNPs. Seedling fresh and dry weights were measured for all treatments. The exposure of plants to 1 mg/l AgNPs resulted in a significant increase in seedling fresh and dry weights compared with control plants, although the exposure to lower and higher concentrations resulted in a decrease in fresh and dry weights. Expression profiling for 22 MADS-box genes was carried out using quantitative real-time...

Characterization of the first Czech sorghum variety Ruzrok tested in Czech RepublicShort Communication

Jiří HERMUTH, Klára KOSOVÁ

Czech J. Genet. Plant Breed., 2017, 53(1):37-44 | DOI: 10.17221/134/2016-CJGPB  

Ruzrok, the first sorghum cultivar of Czech provenance, was selected as a multiline cultivar from sorghum collected in the Bílé Karpaty region in Moravia, Czech Republic. In the present paper, basic plant morphological and yield-related characteristics as well as characteristics related to plant resistance to major abiotic and biotic stress factors are described. The major advantages of Ruzrok consists in its earliness, plant height and high growth rate of plants leading to high biomass production. Ruzrok was registered for its high biomass yield; however, its grains can also be used for flour production and food purposes after the technological removal...

List of field crop varieties registered in the Czech Republic in 2016New Varieties

T. Mezlík

Czech J. Genet. Plant Breed., 2017, 53(1):45-46 | DOI: 10.17221/31/2017-CJGPB