Czech J. Genet. Plant Breed., 2017, 53(1):23-29 | DOI: 10.17221/84/2016-CJGPB

Development of a muskmelon cultivar with improved resistance to gummy stem blight and desired agronomic traits using gene pyramidingOriginal Paper

Ning ZHANG1, Bing-hua XU2, Yan-fei BI1, Qun-feng LOU1, Jin-feng CHEN1, Chun-tao QIAN*,1, Yong-bing ZHANG3, Hong-ping YI3
1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agriculture University, Nanjing, P.R. China
2 Huaiyin Institute of Agricultural Sciences of Xuhuai Region, Jiangsu P.R. China
3 Center of Hami Melon, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang, P.R. China$2

Gummy stem blight (GSB), caused by Didymella bryoniae (Auersw.) Rehm., is a severe disease affecting Cucurbitaceae crops including melons. The resistance of current melon varieties that carry a single Gsb resistance gene is insufficient to protect against the abundant variation of the D. bryoniae isolates. Pyramiding multiple Gsb resistance genes into melon cultivars is an effective way to develop a broad resistance spectrum and to increase the duration of GSB resistance. In this study, two resistance genes (Gsb-4 and Gsb-6) from two resistant accessions, PI482398 and PI420145, were pyramided into one variety using marker-assisted selection (MAS). The donor parent 4598 that contained Gsb-4 and Gsb-6 was hybridized with muskmelon Baipicui to produce BC1F1 and BC2F1. Phenotyping and MAS enabled identification and pyramiding of two Gsb genes in individuals of F1, BC1F1 and BC2F1. Field cultivation showed that individuals carrying both Gsb-4 and Gsb-6 had improved resistance to GSB and improved fruit quality. The results indicated that MAS-based pyramiding is an effective strategy for breeding melon cultivars with increased resistance to GSB.

Keywords: Cucumis melo; Didymella bryoniae; disease resistance breeding; fruit quality; marker-assisted selection

Published: March 31, 2017  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
ZHANG N, Bing-hua X, Yan-fei B, LOU Q, CHEN J, QIAN C, et al.. Development of a muskmelon cultivar with improved resistance to gummy stem blight and desired agronomic traits using gene pyramiding. Czech J. Genet. Plant Breed. 2017;53(1):23-29. doi: 10.17221/84/2016-CJGPB.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Amand P.C., Wehner T.C. (1991): Crop loss to 14 diseases of cucumber in North Carolina from 1983 to 1988. Cucurbit Genetics Cooperative Report, 14: 15-17.
    2. Ashutosh, Sharma P.C., Prakash S., Prakash S., Bhat S.R. (2007): Identification of AFLP markers linked to the male fertility restorer gene of CMS (Moricandia arvensis) Brassica juncea and conversion to SCAR marker. Theoretical and Applied Genetics, 114: 385-92. Go to original source... Go to PubMed...
    3. Frantz J.D., Jahn M.M. (2004): Five independent loci each control monogenic resistance to gummy stem blight in melon (Cucumis melo L.). Theoretical and Applied Genetics, 108: 1033-1038. Go to original source... Go to PubMed...
    4. Gusmini G., Ellington T.L., Wehner T.C. (2003): Mass production of gummy stem blight spores for resistance screening. Cucurbit Genetics Cooperative Report, 26: 26-30.
    5. Gusmini G., Song R.H., Wehner T.C. (2005): New sources of resistance to gummy stem blight in watermelon. Crop Science, 45: 582-588. Go to original source...
    6. Hanson P., Lu S.F., Wang J.F., Chen W., Kenyon L., Tan C.W., Tee K.L., Wang Y.Y., Hsu Y.C., Schafleitner R., Ledesma D., Yang R.Y. (2016): Conventional and molecular marker-assisted selection and pyramiding of genes for multiple disease resistance in tomato. Scientia Horticulturae, 201: 346-354. Go to original source...
    7. Jena K.K., MacKill D.J. (2008): Molecular markers and their use in marker-assisted selection in rice. Crop Science, 48: 1266-1276. Go to original source...
    8. Kang B.C., Yeam I., Jahn M.M. (2005): Genetics of plant virus resistance. Annual Review of Phytopathology, 43: 581-621. Go to original source... Go to PubMed...
    9. Keinath A.P., Farnham M.W., Zitter T.A. (1995): Morphological, pathological, and genetic differentiation of Didymella bryoniae and Phoma ssp. isolated from cucurbits. Phytopathology, 85: 364-369. Go to original source...
    10. Lebeda A. (1985): Dangerous and lesser known pathogen of cucumbers. Záhradníctvo 10, 122-124. (in Czech)
    11. McDonald B.A., Linde C. (2002): The population genetics of plant pathogens and breeding strategies for durable resistance. Euphytica, 124: 163-180. Go to original source...
    12. Mienie C.M.S., Fourie H., Smit M.A., Van Staden J., Botha F.C. (2002): Identification of AFLP markers in soybean linked to resistance to Meloidogyne javanica and conversion to Sequence Characterized Amplified Regions (SCARs). Plant Growth Regulation, 37: 157-166. Go to original source...
    13. Nowicki M., Foolad M.R., Nowakowska M., Kozik E.U. (2012): Potato and tomato late blight caused by Phytophthora infestans: an overview of pathology and resistance breeding. Plant Disease, 96: 4-17. Go to original source... Go to PubMed...
    14. Rogers S.O., Bendich A.J. (1994): Extraction of total cellular DNA from plants, algae and fungi. In: Plant Molecular Biology Manual. Dordrecht, Kluwer Academic Publishers: 1-8. Go to original source...
    15. Sitterly W.R., Keinath A.P. (1996): Gummy stem blight. In: Zitter T.A., Hopkins D.L., Thomas C.E. (eds): Compendium of Cucurbit Diseases. St. Paul, American Phytopathological Society: 27-28.
    16. Sowell G., Jr. (1981): Additional sources of resistance to gummy stem blight of muskmelon. Plant Disease, 65: 253-254. Go to original source...
    17. Suh J.P., Jeung J.U., Noh T.H., Cho Y.C., Park S.H., Shin M.S., Kim C.K., Jena K.K. (2013): Development of breeding lines with three pyramided resistance genes that confer broad-spectrum bacterial blight resistance and their molecular analysis in rice. Rice, 6: 1-11. Go to original source... Go to PubMed...
    18. Tsutsumi C.Y., Silva da N. (2004): Screening of melon populations for resistance to Didymella bryoniae. Brazilian Archives of Biology and Technology, 47: 171-177. Go to original source...
    19. Vidavski F., Czosnek H., Gazit S., Levy D., Lapidot M. (2008): Pyramiding of genes conferring resistance to Tomato yellow leaf curl virus from different wild tomato species. Plant Breeding, 127: 625-631. Go to original source...
    20. Vos P., Hogers R., Bleeker M., Reijans M., Lee T.V., Hornes M., Frijters A., Pot J., Peleman J., Kuiper M., Zabeau M. (1995): AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research, 23: 4407-4414. Go to original source... Go to PubMed...
    21. Wako T., Sakata Y., Sugiyama M., Ohara T., Ishiuji D., Kojima A., Nishimura S. (2002): Identification of melon accessions resistant to gummy stem blight and genetic analysis of the resistance using an efficient technique for seedling test. Acta Horticulturae, 588: 161-164. Go to original source...
    22. Wang H.Y., Qian C.T., Lou L.N., Lou Q.F., Zhang Y.B., Yi H.P., Wu M.Z., Chen J.F. (2012): A SSR marker linked to Gsb-4 loci resistance to gummy stem blight in melon. Acta Horticulturae Sinica, 39: 574-580. (in Chinese)
    23. Wolukau J.N. (2007): Genetic, phylogenetic and molecular studies of melon (Cucumis melo L.) resistance to gummy stem blight (Didymella bryoniae). [PhD. Thesis.] Nanjing, Nanjing Agriculture University.
    24. Ye G.Y., Smith K.F. (2010): Marker-assisted gene pyramiding for cultivar development. Plant Breeding Reviews, 33: 219-256. Go to original source...
    25. Zhang L.J, Fan J.J. (2007): Plant Physiology Experiments Tutorial. Beijing, China Agricultural University Press. (in Chinese)
    26. Zhang Y.P., Kyle M., Anagnostou K., Zitter T.A. (1997): Screening melon (Cucumis melo L.) for resistance to gummy stem blight in the greenhouse and field. HortScience, 32: 117-121. Go to original source...
    27. Zuniga T.L., Jantz J.P., Zitter T.A., Jahn M.M. (1999): Monogenic dominant resistance to gummy stem blight in two melon (Cucumis melo L.) accessions. Plant Disease, 83: 1105-1107. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content