Czech Journal of Genetics and Plant Breeding, 2016 (vol. 52), issue 2
Breeding triploid plants: a reviewReview
Xiling WANG, Zong-Ming CHENG, Shuang ZHI, Fengxiang XU
Czech J. Genet. Plant Breed., 2016, 52(2):41-54 | DOI: 10.17221/151/2015-CJGPB 
Triploid plants have larger organs, greater biomass, and strong stress resistance by preserving relatively larger amounts of photosynthetic energy. The undesirable spread of non-native invasive crop and horticultural plants into natural areas can also be reduced or eliminated by the use of triploids, because they tend to be sterile and seedless. Triploid plants have great economic value and have been useful for developing new agronomic, horticultural, and forestry plant varieties. Because of rapid advances in DNA sequencing technology, triploids may become a focus of genomic research in the future, and will create unprecedented opportunities for discovering...
Evaluation of pea (Pisum sativum L.) germplasm for winter hardiness in Central Anatolia, Turkey, using field and controlled environmentOriginal Paper
AzIze HOMER, Mevlüt ªAHIN, Ümran KÜÇÜKÖZDEMIR
Czech J. Genet. Plant Breed., 2016, 52(2):55-63 | DOI: 10.17221/186/2015-CJGPB
Winter pea can be grown in rotation with cereal crops in Central Anatolia, Turkey. However, winterkill can occur during harsh winters. The objective of this study was to screen pea accessions for winter survival, and identify genotypes with differential winter hardiness for future crop development. The plant material consisted of 58 accessions including local landraces, elite winter cultivars, selected lines and several checks. Twenty-five of them were evaluated under both field and laboratory conditions. The rest of the genotypes were tested under field conditions. Field trials were planted in Haymana, Ankara, and in Ulaº, Sivas, Turkey during the...
Two-dimensional root phenotyping system based on root growth on black filter paper and recirculation micro-irrigationOriginal Paper
Wutthida RATTANAPICHAI, Karel KLEM
Czech J. Genet. Plant Breed., 2016, 52(2):64-70 | DOI: 10.17221/121/2015-CJGPB
Plant root system architecture (RSA) has an important role in crop production, particularly for water and nutrient uptake under limiting conditions. In the last few years, several root phenotyping methods have been developed. Here we present a new technique which has been developed for non-destructive, inexpensive and high-throughput root growth studies and RSA analyses. To illustrate the potential applications, this method was tested in an experiment with nitrogen and phosphorous deficiencies in a nutrient solution, affecting RSA parameters of two spring barley varieties (Bojos and Barke). This technique is based on root growth on vertically positioned...
Molecular characterization of Turkish onion germplasm using SSR markersOriginal Paper
Fatih HANCI, Ali Fuat GÖKÇE
Czech J. Genet. Plant Breed., 2016, 52(2):71-76 | DOI: 10.17221/162/2015-CJGPB
In this study, molecular markers were used for the first time to study the genetic diversity of Turkish onion (Allium cepa L.) accessions. We used 83 turkish landraces, 3 breeding lines and 10 commercial varieties, in total 96 accessions, to study the genetic diversity. Bulked samples of 10 seeds were used to generate a representative profile of the accessions. Variability was evaluated at 46 microsatellite loci. We identified 308 alleles with these markers, out of which 303 were polymorphic. A dendrogram based on the UPGMA analysis grouped the 96 accessions into five main clusters. Dice's similarity coefficient ranged from 0.407 to 0.767...
Enhanced tolerance to low-K+ stress in tobacco plants, that ectopically express the CBL-interacting protein kinase CIPK23 geneShort Communication
Gang XUE, Li-Ming LU, Tie-Zhao YANG, Xiao-Hui LI, Xue-Xia XING, Shi-Xiao XU
Czech J. Genet. Plant Breed., 2016, 52(2):77-82 | DOI: 10.17221/155/2015-CJGPB
Tobacco (Nicotiana tabacum) has a relatively high requirement for potassium (K+). However, the molecular basis of tolerance to low-K+ stresses in tobacco still remains unknown. Here, we report the role of a member of the A. thaliana CBL (calcineurin B-like) interacting protein kinase (CIPK) family, AtCIPK23, in low-K+ stress responses in tobacco. Molecular analyses revealed that the AtCIPK23 gene was successfully transferred into a tobacco cultivar K326 via Agrobacterium tumefaciens-mediated transformation. Overexpression of AtCIPK23 in tobacco resulted in increased...