Czech Journal of Genetics and Plant Breeding, 2016 (vol. 52), issue 1
Genetic analysis and molecular mapping of a leaf rust resistance gene in the wheat line 19HRWSN-129Original Paper
Lingzhi SHI, Zaifeng LI, Xiaodong WANG, Zhanhai KANG, Lin ZHU, Zhikuan REN, Xing LI, Daqun LIU
Czech J. Genet. Plant Breed., 2016, 52(1):1-5 | DOI: 10.17221/167/2015-CJGPB 
The wheat (Triticum aestivum L.) line 19HRWSN-129 from CIMMYT showed resistance to the major Puccinia triticina (Pt) races collected in China. A 144 F2:3 population was generated by crossing the resistant line 19HRWSN-129 with the susceptible line Zhengzhou5389. All the F2:3 plants were phenotyped by inoculating with the Pt race PHGN in the greenhouse. Segregation analysis indicated the presence of a single dominant gene conferring resistance to leaf rust in the wheat line 19HRWSN-129, temporarily designated as Lrshi. Using molecular markers, we mapped this resistance locus on chromosome...
In vitro screening of guava plantlets transformed with endochitinase gene against Fusarium oxysporum f.sp. psidiiOriginal Paper
Maneesh MISHRA, Syed Uzma JALIL, Rupesh Kumar MISHRA, Swati KUMARI, Brajesh Kumar PANDEY
Czech J. Genet. Plant Breed., 2016, 52(1):6-13 | DOI: 10.17221/74/2015-CJGPB
Guava wilt disease is a severe threat to guava growers all over the world. It is caused by the soil-borne fungus Fusarium oxysporum f.sp. psidii. To control the disease, the Trichoderma-endochitinase gene was first introgressed into guava (Psidium guajava L.). The transgenic plantlets were screened in vitro for resistance against the wilt pathogen. Six-months-old genetically transformed plants raised in cocopeat under in vitro conditions were inoculated with a 7-days old culture of F. oxysporum f.sp. psidii. The presence of the pathogen in the cocopeat medium was confirmed...
Genetic effects of high fibre strength breeding lines in crosses with transgenic Bt cotton cultivars (Gossypium hirsutum L.)Original Paper
Fei-Yu TANG, Wang-Cheng MO, Wen-Jun XIAO
Czech J. Genet. Plant Breed., 2016, 52(1):14-21 | DOI: 10.17221/116/2015-CJGPB
Evaluation of genetic effects in cotton (Gossypium hirsutum L.) high fibre strength lines is necessary for the genetic improvement of fibre properties. Six breeding lines with high fibre strength and five transgenic Bt cultivars were diallely crossed. Fibre properties and agronomic traits of 11 parents and resultant 30 F1 hybrids were analyzed by an additive-dominant model with genotype by environment interaction effects. Lint percentage, seed index, fibre length, strength and micronaire were primarily controlled by additive effects. Lint yield was mainly governed by dominance effects. Boll size was equally influenced by additive...
Effects of faba bean (Vicia faba) varieties on the development of Bruchus rufimanusOriginal Paper
Marek SEIDENGLANZ, Igor HUŇADY
Czech J. Genet. Plant Breed., 2016, 52(1):22-29 | DOI: 10.17221/122/2015-CJGPB
The development of Bruchus rufimanus was assessed in randomised field trials from 2005 to 2007 with eight faba bean genotypes (SU 5/13, SU-BT, Mistral, Merkur, Stabil Merlin, Melodie, Divine). Significant differences, reproducible between the years, were found between the faba bean lines. The varieties most attractive to B. rufimanus ovipositing females were SU-BT and Divine. Merkur showed a certain level of non-preference. The highest mortality rates of eggs and first instar larvae in pod valve tissues were recorded in Merkur (34-45%) and Divine (51-55%). The highest mortality rates of larvae and pupae during their development in...
Identification of genes conferring resistance to viral diseases of barley using multiplex PCRShort Communication
Tibor SEDLÁČEK, Pavel MAŘÍK, Jana CHRPOVÁ
Czech J. Genet. Plant Breed., 2016, 52(1):30-33 | DOI: 10.17221/171/2015-CJGPB
This paper describes a rapid and cost-effective assay for identifying economically important barley resistance genes based on multiplex PCR. A newly created reaction mixture is used for detecting the resistance genes rym4, rym5, rym11-b and Ryd2 conferring resistance to barley yellow mosaic viruses and Barley yellow dwarf virus (BYDV). It is possible to identify all these genes using only a single PCR reaction without the necessity for subsequent cutting with restriction endonucleases. This is important for developing resistant barley genotypes inasmuch as it can increase work productivity and reduce costs.
Production of tetraploid plants of Trollius chinensis Bunge induced by colchicineShort Communication
Qin ZHANG, Fan ZHANG, Baohui LI, Luosha ZHANG, Huiqin SHI
Czech J. Genet. Plant Breed., 2016, 52(1):34-38 | DOI: 10.17221/89/2015-CJGPB
Trollius chinensis Bunge is one of the important ornamental and medicinal plants cultivated in China. In this study, the tetraploid plants of Trollius chinensis were successfully induced by colchicine and their morphological, cytological and fertility characteristics were investigated. The results indicated that the tetraploid species of Trollius chinensis (2n = 4x = 32) was efficiently induced from the diploid species (2n = 2x = 16) by colchicine under 0.05% to 0.2% concentrations, with the best induction under treatment combinations of 0.1%, 24h duration and 0.2%, 12h duration. In comparison...
List of field crop varieties registered in the Czech Republic in 2015New Varieties
R. Šafaříková
Czech J. Genet. Plant Breed., 2016, 52(1):39-40 | DOI: 10.17221/4/2016-CJGPB