Czech J. Genet. Plant Breed., 2016, 52(1):30-33 | DOI: 10.17221/171/2015-CJGPB

Identification of genes conferring resistance to viral diseases of barley using multiplex PCRShort Communication

Tibor SEDLÁČEK1, Pavel MAŘÍK1, Jana CHRPOVÁ2
1 Research Centre SELTON, Ltd., Stupice, Czech Republic
2 Crop Research Institute, Prague-Ruzyně, Czech Republic

This paper describes a rapid and cost-effective assay for identifying economically important barley resistance genes based on multiplex PCR. A newly created reaction mixture is used for detecting the resistance genes rym4, rym5, rym11-b and Ryd2 conferring resistance to barley yellow mosaic viruses and Barley yellow dwarf virus (BYDV). It is possible to identify all these genes using only a single PCR reaction without the necessity for subsequent cutting with restriction endonucleases. This is important for developing resistant barley genotypes inasmuch as it can increase work productivity and reduce costs.

Keywords: BaYMV; BaMMV; BaYMV-2; BYDV; Hordeum; resistance genes

Published: March 31, 2016  Show citation

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SEDLÁČEK T, MAŘÍK P, CHRPOVÁ J. Identification of genes conferring resistance to viral diseases of barley using multiplex PCR. Czech J. Genet. Plant Breed. 2016;52(1):30-33. doi: 10.17221/171/2015-CJGPB.
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