Czech Journal of Genetics and Plant Breeding, 2014 (vol. 50), issue 3
Influence of different wheat and Imperata cylindrica genetic backgrounds on haploid induction efficiency in wheat doubled haploid breedingOriginal Paper
Shoukat Ahmad RATHER, Harinder Kumar CHAUDHARY, Vineeta KAILA
Czech J. Genet. Plant Breed., 2014, 50(3):195-200 | DOI: 10.17221/105/2013-CJGPB 
Four Indian and one Japanese accession of Imperata cylindrica were assessed for their influence upon haploid production in F1 generations of 21 wheat crosses (winter × spring, spring × spring and winter × winter) to find an efficient pollen source for haploid induction, which would enhance doubled haploid breeding in bread wheat. The frequency of haploid induction was influenced differently by the wheat and the I. cylindrica genotypes, indicating both maternal and paternal genetic influence on haploid induction. The gene actions controlling the inheritance of haploid induction appeared to be non-additive. Haploid formation...
Common bunt resistance of Czech and European winter wheat cultivars and breeder linesOriginal Paper
Veronika DUMALASOVÁ, Leona LEI©OVÁ-SVOBODOVÁ, Pavel BARTO©
Czech J. Genet. Plant Breed., 2014, 50(3):201-207 | DOI: 10.17221/241/2013-CJGPB
Winter wheat cultivars recently registered in the Czech Republic were tested in three-year field tests for resistance to common bunt. Seeds were inoculated with a mixture of local strains of Tilletia tritici and T. laevis. None of the cultivars displayed a higher level of resistance compared with the resistant checks. The mean percentage of bunted ears in the three test series including checks was 39%. Mean bunt infection in resistant and susceptible checks was 2% and 63%, respectively. In the European Tilletia cooperative test performed in Prague-Ruzyně, thirty-five winter wheat cultivars from six countries were tested during...
Identification of an active 1Ay gene from Triticum turgidum ssp. dicoccoidesOriginal Paper
Zhe-Guang BI, Bi-Hua WU, Xi-Gui HU, Xiao-Hui GUO, Deng-Cai LIU, You-Liang ZHENG
Czech J. Genet. Plant Breed., 2014, 50(3):208-215 | DOI: 10.17221/195/2012-CJGPB
The high molecular weight glutenin subunit (HMW-GS), encoded by the 1Ay gene, unexpressed in common wheat, exists in diploid and tetraploid wheats. An active 1Ay gene was first cloned from wild emmer wheat (T. turgidum ssp. dicoccoides, 2n = 4x = 28, AABB), the oldest species in emmer wheat. Here, a novel subunit encoded by the 1Ay gene (JF519636) present in T. turgidum ssp. dicoccoides line D141 was characterized. The protein had 608 amino acids with six cysteine residues and showed faster electrophoretic mobility than 1Dy12 from common wheat. Compared with previously...
Genetic diversity assessment in winter oilseed rape (Brassica napus L.) collection using AFLP, ISSR and SSR markersOriginal Paper
Lenka HAVLÍČKOVÁ, Eva JOZOVÁ, Andrea RYCHLÁ, Miroslav KLÍMA, Vratislav KUČERA, Vladislav ČURN
Czech J. Genet. Plant Breed., 2014, 50(3):216-225 | DOI: 10.17221/220/2013-CJGPB
The genetic diversity of 94 accessions of winter oilseed rape (Brassica napus L.), representing past and contemporary material utilized in the Czech breeding programmes, was determined using microsatellites (SSRs), ISSRs and AFLPs. All three kinds of markers differed in the range of observed polymorphism and differentiated clearly each accession. Altogether 89 SSR, 1003 AFLP and 53 ISSR markers were evaluated. Their average rates of polymorphic bands were 100%, 53.9% and 90.6%, respectively, indicating high genetic diversity among the accessions. The greatest genetic distance was found by ISSRs (62.3%) whereas significantly lower distances...
Microsatellite analysis indicates the specific genetic basis of Czech bolting garlicOriginal Paper
Jaroslava OVESNÁ, Leona LEI©OVÁ-SVOBODOVÁ, Ladislav KUČERA
Czech J. Genet. Plant Breed., 2014, 50(3):226-234 | DOI: 10.17221/82/2014-CJGPB
Garlic, Allium sativum L., is a vegetable long used for culinary and medical purposes. A certain level of garlic quality is required by the local consumers, which is usually preserved by the varieties grown in that region. The aim was to establish an assay offering fast and inexpensive differentiation of garlic varieties. Length polymorphism of microsatellite loci (SSR, ILP markers) is often used in such a case. No assays have been described earlier. A set of SSR and newly used ILP markers has been assembled and verified. SSR loci ASM53, ASM072, ASA08 and ASA17 were the most polymorphic. Up to 18 alleles were scored per these loci....
Green tissue-specific analysis of a cloned rbcS promoter from Lemna gibbaOriginal Paper
Youru WANG
Czech J. Genet. Plant Breed., 2014, 50(3):235-240 | DOI: 10.17221/200/2013-CJGPB
Many plant genetic engineering taskss require the spatial expression of genes which in turn depends upon the availability of specific promoters. The present paper analyses the green-tissue characteristics of a new L. gibba rbcS promoter driving the expression of the gus gene in transgenic tobacco. A 1491 bp rbcS (small subunit of ribulose bisphosphate carboxylase) promoter was isolated from Lemna gibba. The sequence analysis revealed that this promoter is different from the previously reported rbcS promoter and is named SSU5C. A 1438 bp fragment of the SSU5C promoter was fused with the...
Virulence surveys of wheat leaf rust in the Czech Republic and resistance genes in registered cultivarsScientific Reports
Alena HANZALOVÁ, Pavel BARTO©
Czech J. Genet. Plant Breed., 2014, 50(3):241-246 | DOI: 10.17221/216/2013-CJGPB
The technical report presents a summary of results obtained in the leaf rust race surveys carried out in Czechoslovakia/Czech Republic in the years 1966-2001. The physiologic races were determined using the standard differentials Malakoff (Lr1), Carina (Lr2b), Brevit (Lr2c), Webster (Lr2a), Loros (Lr2c), Mediterranean (Lr3), Hussar (Lr11), Democrat (Lr3) and the additional differential Salzmünder Bartweizen (Lr26). Races 14, 77, 61, 53 and 2 successively dominated in the given period. They mostly appeared in two variants, avirulent and virulent to Lr26. Genes...