Czech Journal of Genetics and Plant Breeding, 2012 (vol. 48), issue 2
Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guideReview
Ugur BAL, Mehran E. SHARIATPANAHI, Antonio J. CASTRO, Delphine EMERY, Christophe CLÉMENT, Maryam DEHESTANI-ARDAKANI, Khadijeh MOZAFFARI, Alisher TOURAEV
Czech J. Genet. Plant Breed., 2012, 48(2):51-60 | DOI: 10.17221/176/2011-CJGPB 
This review describes sources of structures of non-microspore origin observed in anther and microspore cultures. Various characteristics of these structures may cause a wrong diagnosis of these structures as embryos or cell/tissue clusters of microspore origin. Here we suggest such structures to be named as pseudo-embryogenic structures. The introduction of pseudo-embryogenic structures and their origins could be helpful to distinguish them from true microspore-derived structures. Prompted by certain environmental cues, somatic cells existing as a contamination in immature pollen (microspores) cultures can lead to the formation of 'pseudo-embryos'...
Molecular analysis of temporal genetic structuring in pea (Pisum sativum L.) cultivars bred in the Czech Republic and in former Czechoslovakia since the mid-20th centuryOriginal Paper
Jaroslava CIESLAROVÁ, Miroslav HÝBL, Miroslav GRIGA, Petr SMÝKAL
Czech J. Genet. Plant Breed., 2012, 48(2):61-73 | DOI: 10.17221/127/2011-CJGPB
Changes in genetic diversity of peas bred in the Czech Republic and in former Czechoslovakia since the mid-20th century were analysed using 38 molecular marker loci, including retrotransposons and microsatellites, differentiating a total of 84 alleles. Both marker types were comparably effective in revealing the genetic diversity, with a high correlation (r = 0.81), although the pairwise genetic distances of each marker type differed. In total, 175 accessions, selected from the Czech pea gene bank collection and representing the pea cultivars collected or bred in the country, were divided into three groups according to their date...
Enhanced ascorbic acid accumulation through overexpression of dehydroascorbate reductase confers tolerance to methyl viologen and salt stresses in tomatoOriginal Paper
Qingzhu LI, Yansu LI, Chaohan LI, Xianchang YU
Czech J. Genet. Plant Breed., 2012, 48(2):74-86 | DOI: 10.17221/100/2011-CJGPB
As an important antioxidant for plants and humans, L-ascorbic acid (AsA, vitamin C) can scavenge reactive oxygen species (ROS) and can be regenerated from its oxidized form in a reaction catalyzed by dehydroascorbate reductase (DHAR). To analyse the effect of overexpressing DHAR on tomato (Solanum lycopersicum), an expression vector containing potato cytosolic DHAR (DHAR1) or chloroplastic DHAR (DHAR2) cDNA driven by a cauliflower mosaic virus 35S promoter was transferred into tomato plants. Compared with the wild type (WT), DHAR1 overexpression increased DHAR activity and AsA content in...
Genetics of fertility restoration of the A4 cytoplasmic-nuclear male sterility system in pearl milletOriginal Paper
Shashi Kumar GUPTA, Kedar Nath RAI, Mahalingam GOVINDARAJ, Aluri Sambasiva RAO
Czech J. Genet. Plant Breed., 2012, 48(2):87-92 | DOI: 10.17221/164/2011-CJGPB
Inheritance of fertility restoration of the A4 system of cytoplasmic-nuclear male sterility in pearl millet was investigated using six crosses between two diverse male sterile lines (A-lines) and three diverse restorers (R-lines). The segregation pattern of male sterile (S) and male fertile (F) plants observed in F2, and BC1 in two seasons at ICRISAT, Patancheru, indicated the dominant single-gene control of male fertility restoration. The segregation pattern in BC1F2 progenies derived from the fertile BC1 plants evaluated for one season provided further evidence for the single-gene...
Seed emergence of lucerne varieties under different soil conditionsShort Communication
Josef HAKL, Kamila MÁŠKOVÁ, Jaromír ŠANTRŮČEK, Miroslav FÉR
Czech J. Genet. Plant Breed., 2012, 48(2):93-97 | DOI: 10.17221/8/2012-CJGPB
The aim of this study was to examine in greenhouse experiments seed emergence of 14 lucerne varieties (mostly of the Czech origin) under different soil conditions (Luvisol, Chernozem and Cambisol). Soil conditions significantly influenced seed emergence in seven varieties. Their seed emergence was lower in Cambisol (72%) than in Chernozem (80%) and Luvisol (91%). In other varieties the soil effect was not significant. The effect of variety could not be separated from the effect of seed lot in our experiment. However, germination, occurrence of hard seeds, germinative energy and thousand-seed weight were not significantly different between the groups...
Hop varieties Bohemie and Saaz LateNew Varieties
V. Nesvadba
Czech J. Genet. Plant Breed., 2012, 48(2):98-99 | DOI: 10.17221/24/2012-CJGPB