Czech Journal of Genetics and Plant Breeding, 2011 (vol. 47), issue 2

Tracking of powdery mildew and leaf rust resistance genes in Triticum boeoticum and T. urartu, wild relatives of common wheatOriginal Paper

Nelli A. Hovhannisyan, Mohammad Ehsan Dulloo, Aleksandr H. Yesayan, Helmut Knüpffer, Ahmed Amri

Czech J. Genet. Plant Breed., 2011, 47(2):45-57 | DOI: 10.17221/127/2010-CJGPB  

Wild Triticum and Aegilops species are increasingly used in wheat breeding programmes around the world as donors of genes conferring resistance to biotic and abiotic stresses, as well as of genes that contribute to the improvement of grain quality. In the present study, thirty-nine accessions of diploid species with the A genome (Triticum boeoticum and T. urartu) were evaluated for the presence of the genes conferring resistance to powdery mildew (Blumeria graminis) and leaf rust (Puccinia recondita) using both inoculation tests and sequence tagged sites (STS) marker analyses in order to find correspondence...

Resistance to Fusarium head blight in spring barleyOriginal Paper

Jana Chrpová, Václav Šíp, Lenka Štočková, Lenka STEMBERKOVÁ, Ludvík TVARŮŽEK

Czech J. Genet. Plant Breed., 2011, 47(2):58-63 | DOI: 10.17221/129/2010-CJGPB  

Fusarium head blight (FHB) is a fungal disease causing substantial yield and quality losses in barley. Genetic variation in deoxynivalenol (DON) content and and important yield traits in response to FHB were studied in 44 spring barley cultivars for two years following artificial inoculation with Fusarium culmorum under field conditions. The analysis of variance revealed that the largest effect on DON content and simultaneously on the reduction of thousand grain weight and grain weight per spike were due to the environmental conditions of the year, while the visual disease symptoms depended on the cultivars to a larger extent. All these traits...

Presence of the newly designated powdery mildew resistance Landi in some winter barley cultivarsOriginal Paper

Antonín DREISEITL

Czech J. Genet. Plant Breed., 2011, 47(2):64-68 | DOI: 10.17221/14/2011-CJGPB  

In the last two decades, resistance to the powdery mildew pathogen has been investigated in a large number of barleys in the Czech Republic. Several tens of winter barley cultivars were identified with a resistance based on an unknown gene or unknown combinations of resistance genes. In this paper tests on 20 of these cultivars are presented. Thirty-two reference isolates of Blumeria graminis f.sp. hordei were used. All the 20 cultivars shared a particular, previously unknown resistance. Landi was the first cultivar registered with this resistance and it is recommended that this resistance be designated Ln. Isolates virulent to Ln...

Transformation of HBsAg (hepatitis B surface antigen) gene into tomato mediated by Agrobacterium tumefaciensOriginal Paper

Tian Li, Jing Kuan Sun, Zhao Hua Lu, Qing Liu

Czech J. Genet. Plant Breed., 2011, 47(2):69-77 | DOI: 10.17221/5/2011-CJGPB  

The plant expression vector pBRSAg was constructed as suitable for transformation via Agrobacterium-mediated approach. It contains all elements for plant expression, such as CaMV 35S promoter, both left and right border sequence for transferred DNA (T-DNA) in Agrobacterium, plant reporter gene gus, and plant selection marker gene hpt. The recombinant binary vector pBRSAg was transformed into Agrobacterium tumefaciens strains by using the freeze-thaw method. Tomato cotyledon explants were transformed by A. tumefaciens and plants were regenerated on selection medium. GUS staining, PCR and PCR-Southern...

Winter wheat MatyldaNew Varieties

P. Horčička, O. Veškrna, T. Sedláček, J. Chrpová

Czech J. Genet. Plant Breed., 2011, 47(2):78-80 | DOI: 10.17221/46/2011-CJGPB  

Winter barley LesterNew Varieties

P. Mařík

Czech J. Genet. Plant Breed., 2011, 47(2):81-83 | DOI: 10.17221/52/2011-CJGPB