Czech J. Genet. Plant Breed., 2011, 47(2):69-77 | DOI: 10.17221/5/2011-CJGPB

Transformation of HBsAg (hepatitis B surface antigen) gene into tomato mediated by Agrobacterium tumefaciensOriginal Paper

Tian Li, Jing Kuan Sun, Zhao Hua Lu, Qing Liu
Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta, Binzhou University, Binzhou, China

The plant expression vector pBRSAg was constructed as suitable for transformation via Agrobacterium-mediated approach. It contains all elements for plant expression, such as CaMV 35S promoter, both left and right border sequence for transferred DNA (T-DNA) in Agrobacterium, plant reporter gene gus, and plant selection marker gene hpt. The recombinant binary vector pBRSAg was transformed into Agrobacterium tumefaciens strains by using the freeze-thaw method. Tomato cotyledon explants were transformed by A. tumefaciens and plants were regenerated on selection medium. GUS staining, PCR and PCR-Southern analysis of the plants were positive. It was for the first time shown that the HBsAg (hepatitis B surface antigen) gene was introduced into tomato plants. The expression of transgene is under investigation.

Keywords: Agrobacterium; HBsAg gene; tomato; transformation

Published: June 30, 2011  Show citation

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Li T, Sun JK, Lu ZH, Liu Q. Transformation of HBsAg (hepatitis B surface antigen) gene into tomato mediated by Agrobacterium tumefaciens. Czech J. Genet. Plant Breed. 2011;47(2):69-77. doi: 10.17221/5/2011-CJGPB.
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