Czech Journal of Genetics and Plant Breeding, 2010 (vol. 46), issue 4

Index of Volume 46Index

editors

Czech J. Genet. Plant Breed., 2010, 46(4):X1 | DOI: 10.17221/6814-CJGPB  

Cytoplasmic effects on grain resistance to yellowberry in durum wheatOriginal Paper

Fethi Bnejdi, Mourad Saadoun, Mohamed El Gazzah

Czech J. Genet. Plant Breed., 2010, 46(4):145-148 | DOI: 10.17221/18/2010-CJGPB  

Parental, F1, reciprocal F1 (RF1), F2, reciprocal F2 (RF2), BC1P1 and BC1P2 generations of four crosses involving four cultivars of durum wheat (Triticum durum Desf.) were evaluated for grain resistance to yellowberry. Significant differences were reported for F1, F2 and their reciprocals in all crosses. A generation means analysis indicated the inadequacy of additive-dominance model and additive-dominance model considering maternal effects. However, the variation in generation means in the four crosses could be explained...

Allelic variations at Dhn4 and Dhn7 are associated with frost tolerance in barleyOriginal Paper

Ludmila Holková, Pavlína Mikulková, Pavlína Hrstková, Ilja Tom Prášil, Marta Bradáčová, Pavla Prášilová, Oldřich Chloupek

Czech J. Genet. Plant Breed., 2010, 46(4):149-158 | DOI: 10.17221/90/2010-CJGPB  

The sequences of the closely linked barley dehydrin genes Dhn4 and Dhn7 are both known to vary allelically. Here we associated allelic constitution at Dhn4/7 with frost sensitivity across a panel of 30 diverse barley varieties. The combined presence of a 6 bp insertion in exon1 of Dhn4 and a 30 bp deletion in exon1 of Dhn7 was restricted to six-rowed winter and intermediate varieties characterised by relatively higher frost tolerance (12 genotypes; LT50 from -14.2°C to -15.6°C). The alternative combination was present with one exception (sixrowed winter variety Alissa) only in spring and two-rowed...

Development of CAPS marker for identifi cation of rym4 and rym5 alleles conferring resistance to the Barley Yellow Mosaic Virus complex in barleyOriginal Paper

Tibor Sedláček, Pavel Mařík, Jana Chrpová

Czech J. Genet. Plant Breed., 2010, 46(4):159-163 | DOI: 10.17221/7/2010-CJGPB  

In barley, soil-borne viruses of the barley mosaic virus complex (BaYMV, BaMMV, BaYMV-2) are of high importance due to their increased spread, yield losses, and the fact that protection with chemicals is not possible. Concerning resistance breeding, simple, cheap, and robust selection methods are required. Markerassisted selection (MAS) has a great potential to meet this demand. A CAPS marker designed directly for SNPs causing mutations responsible for resistance of rym4/rym5 alleles of the Hv-eIF4E gene was developed in this study. Results showed perfect correspondence between the CAPS marker and the allele identified indirectly...

Alkaloid diversity in relation to breeding for specifi c alkaloids in opium poppy (Papaver somniferum L.)Original Paper

Sudhir Shukla, Hemant Kumar Yadav, Anu Rastogi, Brij Kishore Mishra, Sant Prasad Singh

Czech J. Genet. Plant Breed., 2010, 46(4):164-169 | DOI: 10.17221/96/2009-CJGPB  

Papaver somniferum is a chief source of diverse physiologically active alkaloids, required by the pharmaceutical industry. The present study describes the diversity of the alkaloid spectrum of 122 opium poppy accessions of Indian origin by means of a cluster analysis based on Mahalanobis generalised distances. The accessions could be grouped into 11 clusters according to their relationship between the contents of morphine, codeine, thebaine, narcotine and papaverine in raw opium. The diversity of the alkaloid spectrum of 11 clusters reflected the very low correlations between the contents of the individual alkaloids across the 122 entries,...

Distinguishing grapefruit and pummelo accessions using ISSR markersOriginal Paper

Aydin Uzun, Osman Gulsen, Turgut Yesiloglu, Yildiz Aka-Kacar, Onder Tuzcu

Czech J. Genet. Plant Breed., 2010, 46(4):170-177 | DOI: 10.17221/89/2010-CJGPB  

Grapefruit is the fourth economically most important citrus fruit in the world. In this research Inter-Simple Sequence Repeat (ISSR) markers were used to distinguish twenty-nine grapefruit (Citrus paradisi Macf.), five pummelo (Citrus maxima (Burm.) Merr.) and one Citrus hassaku Hort. Ex Tanaka accessions. Twelve ISSR primers produced a total of 100 fragments and 62 of them were polymorphic. The number of average polymorphic fragments per primer was 5.2. The mean polymorphism information content (PIC) was 0.37. The unweighted pair group method arithmetic average (UPGMA) analysis demonstrated that the accessions had a similarity...

Complete genome sequence of a Brome Mosaic Virus isolate from the Czech RepublicShort Communication

Sebastien Gadiou, Jiban Kumar Kundu

Czech J. Genet. Plant Breed., 2010, 46(4):178-182 | DOI: 10.17221/60/2010-CJGPB  

An isolate of Brome mosaic virus (BMV) was originally isolated from Agropyron repens and maintained in Hordeum vulgare. The full-length genome of this isolate (BMV-CZ) was sequenced. Phylogenetic analysis revealed that BMV-CZ shared a minimum of 95.6% sequence identity, localized in the 5'-UTR of RNA-1 with the other BMV isolates from the database, and a maximum of divergence of 30.8% with Broad bean mottle virus localized in the 5'-UTR of RNA-3. This is the first sequence report of full-length BMV from the Czech Republic.

Winter wheat EllyNew Varieties

P. Horčička, O. Veškrna, T. Sedláček, J. Chrpová

Czech J. Genet. Plant Breed., 2010, 46(4):183-185 | DOI: 10.17221/125/2010-CJGPB  

Green pea variety MorrisNew Varieties

I. Hasalová, R. Trojan, R. Dostálová

Czech J. Genet. Plant Breed., 2010, 46(4):186-187 | DOI: 10.17221/124/2010-CJGPB